MCL1 (NM_182763) Human Untagged Clone

CAT#: SC312742

MCL1 (untagged)-Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCL1
• Synonyms: bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_182763 edited
  CACCCCGTAGGACTGGCCGCCCTAAAACCGTGATAAAGGAGCTGCTCGCCACTTCTCACT
  TCCGCTTCCTTCCAGTAAGGAGTCGGGGTCTTCCCCAGTTTTCTCAGCCAGGCGGCGGCG
  GCGACTGGCAATGTTTGGCCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGG
  GGGGGCCGGCTTGGGGGCCGGCAGCGGCGGCGCCACCCGCCCGGGAGGGCGACTTTTGGC
  TACGGAGAAGGAGGCCTCGGCCCGGCGAGAGATAGGGGGAGGGGAGGCCGGCGCGGTGAT
  TGGCGGAAGCGCCGGCGCAAGCCCCCCGTCCACCCTCACGCCAGACTCCCGGAGGGTCGC
  GCGGCCGCCGCCCATTGGCGCCGAGGTCCCCGACGTCACCGCGACCCCCGCGAGGCTGCT
  TTTCTTCGCGCCCACCCGCCGCGCGGCGCCGCTTGAGGAGATGGAAGCCCCGGCCGCTGA
  CGCCATCATGTCGCCCGAAGAGGAGCTGGACGGGTACGAGCCGGAGCCTCTCGGGAAGCG
  GCCGGCTGTCCTGCCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGA
  CGGGTCACTACCCTCGACGCCGCCGCCAGCAGAGGAGGAGGAGGACGAGTTGTACCGGCA
  GTCGCTGGAGATTATCTCTCGGTACCTTCGGGAGCAGGCCACCGGCGCCAAGGACACAAA
  GCCAATGGGCAGGTCTGGGGCCACCAGCAGGAAGGCGCTGGAGACCTTACGACGGGTTGG
  GGATGGCGTGCAGCGCAACCACGAGACGGCCTTCCAAGGATGGGTTTGTGGAGTTCTTCC
  ATGTAGAGGACCTAGAAGGTGGCATCAGGAATGTGCTGCTGGCTTTTGCAGGTGTTGCTG
  GAGTAGGAGCTGGTTTGGCATATCTAATAAGATAGCCTTACTGTAAGTGCAATAGTTGAC
  TTTTAACCAACCACCACCACCACCAAAACCAGTTTATGCAGTTGGACTCCAAGCTGTAAC
  TTCCTAGAGTTGCACCCTAGCAACCTAGCCAGAAAAGCAAGTGGCAAGAGGATTATGGCT
  AACAAGAATAAATACATGGGAAGAGTGCTCCCCATTGATTGAAGAGTCACTGTCTGAAAG
  AAGCAAAGTTCAGTTTCAGCAACAAACAAACTTTGTTTGGGAAGCTATGGAGGAGGACTT
  TTAGATTTAGTGAAGATGGTAGGGTGGAAAGACTTAATTTCCTTGTTGAGAACAGGAAAG
  TGGCCAGTAGCCAGGCAAGTCATAGAATTGATTACCCGCCGAATTCATTAATTTACTGTA
  GTGTTAAGAGAAGCACTAAGAATGCCAGTGACCTGTGTAAAAGTTACAAGTAATAGAACT
  ATGACTGTAAGCCTCAGTACTGTACAAGGGAAGCTTTTCCTCTCTCTAATTAGCTTTCCC
  AGTATACTTCTTAGAAAGTCCAAGTGTTCAGGACTTTTATACCTGTTATACTTTGGCTTG
  GTTTCCATGATTCTTACTTTATTAGCCTAGTTTATCACCAATAATACTTGACGGAAGGCT
  CAGTAATTAGTTATGAATATGGATATCCTCAATTCTTAAGACAGCTTGTAAATGTATTTG
  TAAAAATTGTATATATTTTTACAGAAAGTCTATTTCTTTGAAACGAAGGAAGTATCGAAT
  TTACATTAGTTTTTTTCATACCCTTTTGAACTTTGCAACTTCCGTAATTAGGAACCTGTT
  TCTTACAGCTTTTCTATGCTAAACTTTGTTCTGTTCAGTTCTAGAGTGTATACAGAACGA
  ATTGATGTGTAACTGTATGCAGACTGGTTGTAGTGGAACAAATCTGATAACTATGCAGGT
  TTAAATTTTCTTATCTGATTTTGGTAAGTATTCCTTAGATAGGTTTTTCTTTGAAAACCT
  GGGATTGAGAGGTTGATGAATGGAAATTCTTTCACTTCATTATATGCAAGTTTTCAATAA
  TTAGGTCTAAGTGGAGTTTTAAGGTTACTGATGACTTACAAATAATGGGCTCTGATTGGG
  CAATACTCATTTGAGTTCCTTCCATTTGACCTAATTTAACTGGTGAAATTTAAAGTGAAT
  TCATGGGCTCATCTTTAAAGCTTTTACTAAAAGATTTTCAGCTGAATGGAACTCATTAGC
  TGTGTGCATATAAAAAGATCACATCAGGTGGATGGAGAGACATTTGATCCCTTGTTTGCT
  TAATAAATTATAAAATGATGGCTTGGAAAAGCAGGCTAGTCTAACCATGGTGCTATTATT
  AGGCTTGCTTGTTACACACACAGGTCTAAGCCTAGTATGTCAATAAAGCAAATACTTACT
  GTTTTGTTTCTATTAATGATTCCCAAACCTTGTTGCAAGTTTTTGCATTGGCATCTTTGG
  ATTTCAGTCTTGATGTTTGTTCTATCAGACTTAACCTTTTATTTCCTGTCCTTCCTTGAA
  ATTGCTGATTGTTCTGCTCCCTCTACAGATATTTATATCAATTCCTACAGCTTTCCCCTG
  CCATCCCTGAACTCTTTCTAGCCCTTTTAGATTTTGGCACTGTGAAACCCCTGCTGGAAA
  CCTGAGTGACCCTCCCTCCCCACCAAGAGTCCACAGACCTTTCATCTTTCACGAACTTGA
  TCCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTGAGAGGTGGG
  AGGAAGTCCCTTTTCCTTGGACTGGTATCTTTTCAACTATTGTTTTATCCTGTCTTTGGG
  GGCAATGTGTCAAAAGTCCCCTCAGGAATTTTCAGAGGAAAGAACATTTTATGAGGCTTT
  CTCTAAAGTTTCCTTTGTATAGGAGTATGCTCACTTAAATTTACAGAAAGAGGTGAGCTG
  TGTTAAACCTCAGAGTTTAAAAGCTACTGATAAACTGAAGAAAGTGTCTATATTGGAACT
  AGGGTCATTTGAAAGCTTCAGTCTCGGAACATGACCTTTAGTCTGTGGACTCCATTTAAA
  AATAGGTATGAATAAGATGACTAAGAATGTAATGGGGAAGAACTGCCCTGCCTGCCCATC
  TCAGAGCCATAAGGTCATCTTTGCTAGAGCTATTTTTACCTATGTATTTATCGTTCTTGA
  TCATAAGCCGCTTATTTATATCATGTATCTCTAAGGACCTAAAAGCACTTTATGTAGTTT
  TTAATTAATCTTAAGATCTGGTTACGGTAACTAAAAAAGCCTGTCTGCCAAATCCAGTGG
  AAACAAGTGCATAGATGTGAATTGGTTTTTAGGGGCCCCACTTCCCAATTCATTAGGTAT
  GACTGTGGAAATACAGACAAGGATCTTAGTTGATATTTTGGGCTTGGGGCAGTGAGGGCT
  TAGGACACCCCAAGTGGTTTGGGAAAGGAGGAGGGGAGTGGTGGGTTTATAGGGGGAGGA
  GGAGGCAGGTGGTCTAAGTGCTGACTGGCTACGTAGTTCGGGCAAATCCTCCAAAAGGGA
  AAGGGAGGATTTGCTTAGAAGGATGGCGCTCCCAGTGACTACTTTTTGACTTCTGTTTGT
  CTTACGCTTCTCTCAGGGAAAAACATGCAGTCCTCTAGTGTTTCATGTACATTCTGTGGG
  GGGTGAACACCTTGGTTCTGGTTAAACAGCTGTACTTTTGATAGCTGTGCCAGGAAGGGT
  TAGGACCAACTACAAATTAATGTTGGTTGTCAAATGTNNAGTGTGTTTNNNCCCTAAACT
  TTCTGTTTTTTTCCTGAGAAAAAAAAATAAATCTTTTATTCAAAAAAAAAAAAAAAAAAM
  TCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGAACCCGGGTGGCATNC
  CCTGTGACCCCTNCCNCCAGTGCTCNCNCTGGNCCTGGNAAGTGCCATCNCCAAAGGGGC
  NNNGAAAANNNNNNNNNNNNNNNNN
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182763 unedited
  GACGCCCCAGAGAGAGGAGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTA
  CCTTCGGGAGCAGGCCACCGGCGCCAAGGACACAAAGCCAATGGGCAGGTCTGGGGCCAC
  CAGCAGGAAGGCGCTGGAGACCTTACGACGGGTTGGGGATGGCGTGCAGCGCAACCACGA
  GACGGCCTTCCAAGGATGGGTTTGTGGAGTTCTTCCATGTAGAGGACCTAGAAGGTGGCA
  TCAGGAATGTGCTGCTGGCTTTTGCAGGTGTTGCTGGAGTAGGAGCTGGTTTGGCATATC
  TAATAAGATAGCCTTACTGTAAGTGCAATAGTTGACTTTTAACCAACCACCACCACCACC
  AAAACCAGTTTATGCAGTTGGACTCCAAGCTGTAACTTCCTAGAGTTGCACCCTAGCAAC
  CTAGCCAGAAAAGCAAGTGGCAAGAGGATTATGGCTAACAAGAATAAATACATGGGAAGA
  GTGCTCCCCATTGATTGAAGAGTCACTGTCTGAAAGAAGCAAAGTTCAGTTTCAGCAACA
  AACAAACTTTGTTTGGGAAGCTATGGAGGAGGACTTTTAGATTTAGTGAAGATGGTAGGG
  TGGAAAGACTTAATTTCCTTGTTGAGAACAGGAAAGTGGCCAGTAGCCAGGCAAGTCATA
  GAATTGATTACCCGCCGAATTCATTAATTTACTGTAGTGTTAAGAGAAGCACTAAGAATG
  CCAGTGACCTGTGTAAAAGTTACAAGTAATAGAACTATGACTGTAAGCCTCAGTACTGTA
  CAAGGGAAGCTTTTCCTCTCTCTAATTAGCTTTCCCAGTATACTTCTTAGAAAGTCCAAG
  TGTTCAGACTTTATACCTGTATACTTTGGCTTGGTTCCATGATTCTACTTTATTAGCCCT
  AGTTTATCACCCAATAATACCTTGACC
  
• Restriction Sites: Please inquire
• ACCN: NM_182763
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182763.1, NP_877495.1
• RefSeq Size: 3772 bp
• RefSeq ORF: 816 bp
• Locus ID: 4170
• Cytogenetics: 1q21.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]'
  Transcript Variant: This variant (2), also known as MCL-1S (short), lacks an alternate exon that causes a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1.