SEPTIN4 (NM_080415) Human Untagged Clone

CAT#: SC312764

41521 (untagged)-Human septin 4 (SEPT4), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEPTIN4
• Synonyms: ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_080415 edited
  GGGTGACGGCGGTGCTGCGAGGTCGGCGCGCAGCTCCGCCGCGGGTCGCTCGGGCGCTGT
  CCAGGCGGAGCCGGCCCCGCCCGGGCTGCAGCCATGATCAAGCGTTTCCTGGAGGACACC
  ACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGATTTCTCAGGAAATGCGAGCTGCCAC
  CCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAAGTCCCGGAGCCAAGGCCCCAGGCC
  CCGGACCTCTATGATGATGACCTGGAGTTCAGACCCCCCTCGCGGCCCCAGTCCTCTGAC
  AACCAGCAGTACTTCTGTGCCCCAGCCCCTCTCAGCCCATCTGCCAGGCCCCGCAGCCCA
  TGGGGCAAGCTTGATCCCTATGATTCCTCTGAGGATGACAAGGAGTATGTGGGCTTTGCA
  ACCCTCCCCAACCAAGTCCACCGAAAGTCCGTGAAGAAAGGCTTTGACTTTACCCTCATG
  GTGGCAGGAGAGTCTGGCCTGGGCAAATCCACACTTGTCAATAGCCTCTTCCTCACTGAT
  CTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCATGCAAACTGTGGAGATC
  ACTAAGCATGCAGTGGACATAGAAGAGAAGGGTGTGAGGCTGCGGCTCACCATTGTGGAC
  ACACCAGGTTTTGGGGATGCAGTCAACAACACAGAGTGCTGGAAGCCTGTGGCAGAATAC
  ATTGATCAGCAGTTTGAGCAGTATTTCCGAGACGAGAGTGGCCTGAACCGAAAGAACATC
  CAAGACAACAGGGTGCACTGCTGCCTGTACTTTATCTCACCCTTCGGCCATGGGTATGGT
  CCAAGCCTGAGGCTCCTGGCACCACCGGGTGCTGTCAAGGGAACAGGCCAAGAGCACCAG
  GGGCAGGGCTGCCACTAGCAGGTGGTCACAGGTTCCTGTTCCCCAGGCTCCGGCCATTGG
  ATGTTGAATTCATGAAGGCCCTGCATCAGCGGGTCAACATCGTGCCTATCCTGGCTAAGG
  CAGACACACTGACACCTCCCGAAGTGGACCACAAGAAACGCAAAATCCGGGAGGAGATTG
  AGCATTTTGGAATCAAGATCTATCAATTCCCAGACTGTGACTCTGATGAGGATGAGGACT
  TCAAATTGCAGGACCAAGCCCTAAAGGAAAGCATCCCATTTGCAGTAATTGGCAGCAACA
  CTGTAGTAGAGGCCAGAGGGCGGCGAGTTCGGGGTCGACTCTACCCCTGGGGCATCGTGG
  AAGGACCTGAAGGATGTGACGCGGGAGACACATTATGAGAACTACCGGGCACAGTGCATC
  CAGAGCATGACCCGCCTGGTGGTGAAGGAACGGAATCGCAACAAACTGACTCGGGAAAGT
  GGTACCGACTTCCCCATCCCTGCTGTCCCACCAGGGACAGATCCAGAAACTGAGAAGCTT
  ATCCGAGAGAAAGATGAGGAGCTGCGGCGGATGCAGGAGATGCTACACAAAATACAAAAA
  CAGATGAAGGAGAACTATTAACTGGCTTTCAGCCCTGGATATTTAAATCTCCTCCTCTTC
  TTCCTGTCCATGCCGGCCCCTCCCAGCACCAGCTCTGCTCAGGCCCCTTCAGCTACTGCC
  ACTTCGCCTTACATCCCTGCTGACTGCCCAGAGACTCAGAGGAAATAAAGTTTAATAAAT
  CTGTAGTGAGAATCCATCAGGCAACACAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080415 unedited
  GTAGGACTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGGGGTGACGGCGGTGCTGCGAGGTCGGCGCGCAGCTCCGCCGCGGGT
  CGCTCGGGCGCTGTCCAGGCGGAGCCGGCCCCGCCCGGGCTGCAGCCATGATCAAGCGTT
  TCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGATTTCTCAGGAA
  ATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAAGTCCCGGAGC
  CAAGGCCCCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGACCCCCCTCGCGGC
  CCCAGTCCTCTGACAACCAGCAGTACTTCTGTGCCCCAGCCCCTCTCAGCCCATCTGCCA
  GGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTCCTCTGAGGATGACAAGGAGT
  ATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTGAAGAAAGGCTTTG
  ACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGGGCAAATCCACACTTGTCAATAGCC
  TCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCATGC
  AAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGGTGTGAGGCTGCGGC
  TCACCATTGTGGACACACCAGGTTTTGGGGATGCAGTCAACAACACAGAGTGCTGGAAGC
  CTGTGGCAGATACATTGATCAGCAGTTTGAGCAGTATTTCCCGAGACGAGAGTGGCCTGA
  ACCGAAAGACATCCAAGACACAGGTGCACTGCTGCCTGTACTTTATTCTCACCCTTCGGC
  CATGGGTTATGGTTCCAGGCCTGAGGCTTCCTGGCAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080415 unedited
  CGTGATGCACCTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCAACAAATTTAT
  AAAATTTTATTTCCCCTAATTCTCGGGGCATTCCCCAGGGATGTAAGGCAAAGTGGCATT
  ACCTAAAGGGGCCTGACCAAACCTGGGGCGGGGAGGGGCCGGCTTGGACAGAAAAAAAAG
  AAAAAAATTTAAATTTCCGGGGCTAAAACCCATTTAAAATTTCCCCTTCATCGGTTTTTG
  TTTTTGGGGAACCATCTCCGGCACCCCCCGCACCTCCTCATTTTTTTTTCGAATAACCTT
  CTAATTTTCTGAATCTGCCCCGGGGGGAACACCGGGGATGGGAAATTCGGTCCCCTTTTC
  CCAATTCATTTTGTTGCAATTCCTTTCTTTCACCACCAGGCGGGTCATGCTTTGAATGCA
  TTGTCCCCGGTATTTCTAAAAATGTGTCTCCCGGTAAAACCTTAAGGTCCTTCCAAATGC
  CCAGGGGTAAAGTCGACCCCGAACTCGCCGCCCTCTGGCCTTTATAAAGTGTGGCTGCCA
  TTACTGAAATGGGGACGCTTTCCTTTAGGGCTTGGTCCGCATTTGAATTCCTCATCCTCA
  TCAGAGTACAGTCTGGGAATTGATATATCTGATCAAAATGCTCATCTCTCCCGGATTTGC
  GTTTCTTGGGTCACTTCGGGAGTGTCAGTGTGTCTGCCTTACCAGAAGCCATGTGACCCG
  TGATGCGGCCTATGGAATCACATCCATGCCGAACCTGGAAACAGAACTGT
  
• Restriction Sites: Please inquire
• ACCN: NM_080415
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080415.1, NP_536340.1
• RefSeq Size: 1911 bp
• RefSeq ORF: 825 bp
• Locus ID: 5414
• Cytogenetics: 17q22
• Domains: GTP_CDC
• Gene Summary: 'This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region and initiates translation at an alternate start codon, compared to variant 7. This transcript contains an early translation termination site, rendering this transcript a candidate for nonsense-mediated mRNA decay (NMD). However, the encoded protein (isoform 2, also known as ARTS, SEPT4_i2), with distinct N- and C-termini compared to isoform 5, has been shown to be expressed in vivo, and characterized as a mitochondrial pro-apoptotic, tumor suppressor protein having a role in cancer (PMIDs: 11146656, 15254396).