Tapasin (TAPBP) (NM_172208) Human Untagged Clone

CAT#: SC312855

TAPBP (untagged)-Human TAP binding protein (tapasin) (TAPBP), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAPBP
• Synonyms: NGS17; TAPA; TPN; TPSN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_172208, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGTCCCTGTCTCTGCTCCTCGCTGTGGCTTTGGGCCTGGCGACCGCCGTCTCAGCAGGACCCGCGG
  TGATCGAGTGTTGGTTCGTGGAGGATGCGAGCGGAAAGGGCCTGGCCAAGAGACCCGGTGCACTGCTGTT
  GCGCCAGGGACCGGGGGAACCGCCGCCCCGGCCGGACCTCGACCCTGAGCTCTATCTCAGTGTACACGAC
  CCCGCGGGCGCCCTCCAGGCTGCCTTCAGGCGGTATCCCCGGGGCGCCCCCGCACCACACTGCGAGATGA
  GCCGCTTCGTGCCTCTCCCCGCCTCTGCGAAATGGGCCAGCGGCCTGACCCCCGCGCAGAACTGCCCGCG
  GGCCCTGGATGGGGCTTGGCTGATGGTCAGCATATCCAGCCCAGTCCTCAGCCTCTCCAGCCTCTTGCGA
  CCACAGCCAGAGCCTCAGCAGGAGCCTGTTCTCATCACCATGGCAACAGTGGTACTGACTGTCCTCACCC
  ACACCCCTGCCCCTCGAGTGAGACTGGGACAAGATGCTCTGCTGGACTTGAGCTTTGCCTACATGCCCCC
  CACCTCCGAGGCCGCCTCATCTCTGGCTCCGGGTCCCCCTCCCTTTGGGCTAGAGTGGCGACGCCAGCAC
  CTGGGTAAGGGACATCTGCTCCTGGCTGCAACTCCTGGGCTGAATGGCCAGATGCCAGCAGCCCAAGAAG
  GGGCCGTGGCATTTGCTGCTTGGGATGATGATGAGCCATGGGGCCCATGGACCGGAAATGGGACCTTCTG
  GCTGCCTACAGTTCAACCCTTTCAGGAGGGCACCTATCTGGCCACCATACACCTGCCATACCTGCAAGGA
  CAGGTCACCCTGGAGCTTGCTGTGTACAAACCCCCCAAAGTGTCCCTGATGCCAGCAACCCTTGCACGGG
  CCGCCCCAGGGGAGGCACCCCCGGAATTGCTCTGCCTTGTGTCCCACTTCTACCCTTCTGGGGGCCTGGA
  GGTGGAGTGGGAACTCCGGGGTGGCCCAGGGGGCCGCTCTCAGAAGGCCGAGGGGCAGAGGTGGCTCTCG
  GCCCTGCGCCACCATTCCGATGGCTCTGTCAGCCTCTCTGGGCACTTGCAGCCGCCCCCAGTCACCACTG
  AGCAGCATGGGGCACGCTATGCCTGTCGAATTCACCATCCCAGCCTGCCTGCCTCGGGGCGCAGCGCTGA
  GGTCACCCTGGAGGTAGCAGGTCTTTCAGGGCCCTCCCTTGAGGACAGCGTAGGCCTTTTCCTGTCTGCC
  TTTCTTCTGCTTGGGCTCTTCAAGGCACTGGGCTGGGCTGCTGTCTACCTGTCCACCTGCAAGGATTCAA
  AGAAGGTACAGTGCTCCACCTCTCTGTATCTTTCCCTTGTCACTTTATCTCCTCATCCTATCTCAAAACC
  CATGGAGGGAGGCTGCTGGTGTGGTAGGCAGAACCTAGGCTTGGAATTCACACTGATCTGGGTTAAAACC
  TGGCACTATATCCTAACTGTAGGACTCTTTGAGCATGCTACTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_172208
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172208.2, NP_757345.2
• RefSeq Size: 2003 bp
• RefSeq ORF: 1515 bp
• Locus ID: 6892
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Antigen processing and presentation
• Gene Summary: 'This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.