PHF1 (NM_002636) Human Untagged Clone

CAT#: SC313557

PHF1 (untagged)-Human PHD finger protein 1 (PHF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHF1
• Synonyms: hPHF1; MTF2L2; PCL1; PHF2; TDRD19C
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002636, the custom clone sequence may differ by one or more nucleotides
  ATGGCGCAGCCCCCCCGGCTGAGCCGCTCTGGTGCCTCCTCACTTTGGGACCCAGCTTCT
  CCTGCTCCCACCTCTGGCCCCAGGCCTCGGCTTTGGGAGGGTCAAGATGTGCTGGCCAGA
  TGGACTGATGGGCTGCTATACTTGGGTACCATCAAAAAGGTGGACAGTGCTAGGGAGGTG
  TGTCTGGTCCAGTTTGAGGATGATTCGCAGTTTCTGGTTCTATGGAAAGACATTAGCCCT
  GCTGCCCTCCCTGGAGAGGAACTCCTCTGTTGTGTCTGTCGCTCTGAGACTGTGGTCCCT
  GGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCATGCTTATCACCAGGACTGCCATGTT
  CCCAGGGCTCCAGCCCCTGGAGAGGGAGAGGGCACATCCTGGGTATGCCGCCAGTGTGTC
  TTTGCGATCGCCACCAAGAGGGGAGGTGCCCTGAAGAAGGGCCCCTATGCCCGGGCCATG
  CTGGGTATGAAGCTTTCTCTGCCATATGGACTGAAGGGGCTGGACTGGGATGCTGGACAT
  CTGAGCAACCGACAGCAGAGTTACTGTTACTGTGGTGGCCCTGGGGAGTGGAACCTGAAA
  ATGCTGCAGTGCCGGAGCTGCCTGCAGTGGTTCCATGAGGCCTGCACCCAGTGTCTGAGC
  AAGCCCCTCCTCTATGGGGACAGGTTCTATGAATTTGAATGCTGTGTGTGTCGCGGGGGC
  CCTGAGAAAGTCCGGAGACTACAGCTTCGCTGGGTGGATGTGGCCCATCTTGTCCTGTAT
  CACCTCAGTGTTTGCTGTAAGAAGAAATACTTTGATTTTGATCGTGAGATCCTCCCCTTC
  ACTTCTGAGAATTGGGACAGTTTGCTCCTGGGGGAGCTTTCAGACACCCCCAAAGGAGAA
  CGTTCTTCCAAGCTCCTCTCTGCTCTTAACAGCCACAAGGACCGTTTCATTTCAGGGAGA
  GAGATTAAGAAGAGGAAATGTTTGTTTGGTCTCCATGCTCGGATGCCTCCCCCTGTGGAG
  CCCCCTACTGGAGATGGAGCACTCACCAGGGCAGGGCCCTGGGGGAGGGGTCTCACGTCC
  CCTGGGGAAGCGCCGGAGGCCGGAGCCAGAGCCCCTGAGGAGGAGGCAGAAGGGGAAAGT
  GGAGGAGCTGGGGCCACCCTCAGCAGTGCGCAATCAGCCCGAGCCCCAGGAGCAGAGGGA
  GCGGGCTCATCTGCAGAGGGCACTGCAGCAGCCCCATCCGGATGTTTGCTTCCTTCCACC
  CTTCTGCCAGCACCGCAGGGACCTCTGGGGACAGTGGACCCCCAGACAGGTCACCCCTGG
  AACTTCACATTGGTTTCCCCACAGACATCCCTAAAAGTGCCCCCCACTCGA
  
• Restriction Sites: Please inquire
• ACCN: NM_002636
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002636.3, NP_002627.1
• RefSeq Size: 2008 bp
• RefSeq ORF: 1374 bp
• Locus ID: 5252
• Cytogenetics: 6p21.32
• Domains: PHD, TUDOR
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]'
  Transcript Variant: This variant (1), uses an alternate splice site and lacks an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 2. The resulting isoform (a) has a shorter and distinct C-terminus, compared to isoform b.