PDCD4 (NM_145341) Human Untagged Clone

CAT#: SC313564

PDCD4 (untagged)-Human programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDCD4
• Synonyms: H731
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_145341 edited
  ATGACCAAATATCCTGATAACTTAAGTGACTCTCTCTTTTCCGGTGATGAAGAAAATGCT
  GGGACTGAGGAAGTAAAGAATGAAATAAATGGAAATTGGATTTCAGCATCCTCCATTAAC
  GAAGCTAGAATTAATGCCAAGGCAAAAAGGCGACTAAGGAAAAACTCATCCCGGGACTCT
  GGCAGAGGCGATTCGGTCAGCGACAGTGGGAGTGACGCCCTTAGAAGTGGATTAACTGTG
  CCAACCAGTCCAAAGGGAAGGTTGCTGGATAGGCGATCCAGATCTGGGAAAGGAAGGGGA
  CTACCAAAGAAAGGTGGTGCAGGAGGCAAAGGTGTCTGGGGTACACCTGGACAGGTGTAT
  GATGTGGAGGAGGTGGATGTGAAAGATCCTAACTATGATGATGACCAGGAGAACTGTGTT
  TATGAAACTGTAGTTTTGCCTTTGGATGAAAGGGCATTTGAGAAGACTTTAACACCAATC
  ATACAGGAATATTTTGAGCATGGAGATACTAATGAAGTTGCGGAAATGTTAAGAGATTTA
  AATCTTGGTGAAATGAAAAGTGGAGTACCAGTGTTGGCAGTATCCTTAGCATTGGAGGGG
  AAGGCTAGTCATAGAGAGATGACATCTAAGCTTCTTTCTGACCTTTGTGGGACAGTAATG
  AGCACAACTGATGTGGAAAAATCATTTGATAAATTGTTGAAAGATCTACCTGAATTAGCA
  CTGGATACTCCTAGAGCACCACAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGAT
  GGAATTTTATGTAATACCTATATTGATAGTTACAAAGGAACTGTAGATTGTGTGCAGGCT
  AGAGCTGCTCTGGATAAGGCTACCGTGCTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAA
  GATAGTGTGTGGGGCTCTGGAGGTGGGCAGCAATCTGTCAATCACCTTGTTAAAGAGATT
  GATATGCTGCTGAAAGAATATTTACTCTCTGGAGACATATCTGAAGCTGAACATTGCCTT
  AAGGAACTGGAAGTACCTCATTTTCACCATGAGCTTGTATATGAAGCTATTATAATGGTT
  TTAGAGTCAACTGGAGAAAGTACATTTAAGATGATTTTGGATTTATTAAAGTCCCTTTGG
  AAGTCTTCTACCATTACTGTAGACCAAATGAAAAGAGGTTATGAGAGAATTTACAATGAA
  ATTCCGGACATTAATCTGGATGTCCCACATTCATACTCTGTGCTGGAGCGGTTTGTAGAA
  GAATGTTTTCAGGCTGGAATAATTTCCAAACAACTCAGAGATCTTTGTCCTTCAAGGGGC
  AGAAAGCGTTTTGTAAGCGAAGGAGATGGAGGTCGTCTTAAACCAGAGAGCTACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145341 unedited
  GAAAATCGTTCTTCAATTTGTNAATACCAACCTCACTATAGGGGCGGGCCGCGNATATTC
  GGCACGAGGGTTCGGGGCCGGCTGACCAGNAACCTGGGCGAGCAGCGGCGGGGGCCCGAG
  GGATTCTGAAGGAAGATTTCCATTAGGTAATTTGTTTAATCAGTGCAAGCGAAATTAAGG
  GAAAATGGATGTAGAAAATGAGCAGATACTGAATGTAAACCCTGCAGGGTATTTTCCCTA
  ATTCTCCATGGTGCTTCAATAGCATGTTATTATCATAAAAATGAACAGTTTTGTGGAATA
  GATGACCAAATATCCTGATAACTTAAGTGACTCTCTCTTTTCCGGTGATGAAGAAAATGC
  TGGGACTGAGGAAGTAAAGAATGAAATAAATGGAAATTGGATTTCAGCATCCTCCATTAA
  CGAAGCTAGAATTAATGCCAAGGCAAAAAGGCGACTAAGGAAAAACTCATCCCGGGACTC
  TGGCAGAGGCGATTCGGTCAGCGACAGTGGGAGTGACGCCCTTAGAAGTGGATTAACTGT
  GCCAACCAGTCCAAAGGGAAGGTTGCTGGATAGGCGATCCAGATCTGGGAAAGGAAGGGG
  ACTACCAAAGAAAGGTGGTGCAGGAGGCAAAGGTGTCTGGGGTACACCTGGACAGGTGTA
  TGATTGGAAGAGTTGGGGATTTTTNNAAAGATTCTAACTATGATGATGACCANGNAGAAC
  TGTGTTTATGAAACTGGTATTTTGCCTTTGGATGAAAGGGCATTTGAAAAGACTTTAACA
  CCCATCCTACAGGAATTTTTTGAGCATGGAAATACTAATGGAGTTGCGGAAATGTTAAGA
  AGATTTAATCTTGGTGAAATTGAAAGTGGGATTCCCGTGTTTGGCAGTTTCCTTAACTTG
  GAGGGGGAAGGCTAGTTCATAAAAAA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_145341 unedited
  ATCTTTAGAGCATGGAGAACTAATGAAGTTGCGGATATGTTAAGAGATTTAAATCTTGGT
  GAAATGAAAAGTGGAGTACCAGTGTTGGCAGTATCCTTAGCATTGGAGGGGAAGGCTAGT
  CATAGAGAGATGACATCTAAGCTTCTTTCTGACCTTTGTGGGACAGTAATGAGCACAACT
  GATGTGGAAAAATCATTTGATAAATTGTTGAAAGATCTACCTGAATTAGCACTGGATACT
  CCTAGAGCACCACAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGATGGAATTTTA
  TGTAATACCTATATTGATAGTTACAAAGGAACTGTAGATTGTGTGCAGGCTAGAGCTGCT
  CTGGATAAGGCTACCGTGCTTCTGAGTATGTCTAAAGGTGGAAAGCGTAAAGATAGTGTG
  TGGGGCTCTGGAGGTGGGCAGCAATCTGTCAATCACCTTGTTAAAGAGATTGATATGCTG
  CTGAAAGAATATTTACTCTCTGGAGACATATCTGAAGCTGAACATTGCCTTAAGGAACTG
  GAAGTACCTCATTTTCACCATGAGCTTGTATATGAAGCTATTATAATGGTTTTAGAGTCA
  ACTGGAGAAAGTACATTTAAGATGATTTTGGATTTATTAAAGTCCCTTTGGAAGTCTTCT
  ACCATTACTGTAGACCAAATGAAAAGAGGTTATGAGAGAATTTACAATGAAATTCCGGAC
  ATTAATCTGGATGTCCCACATTCATACTCTGTGCTGGAGCGGTTTGTAGAAGAATGTTTT
  CAGGCTGGAATAATTTCCAAACAACTCAGAGATCTTTGTCCTTCATGGGGCAGAAAGCGT
  TTTGTAGCGAAGAGATGGAGTCGTCTTAACCCAGGAGAGCTACTGAATATTAAGAACTCT
  TGCAGTCTTAGAATGTTATAAAAAATATATATTCTGAATTGTAAGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145341
• ORF Size: 1377 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.

Reference Data

• RefSeq: NM_145341.2, NP_663314.1
• RefSeq Size: 3675
• RefSeq ORF: 1377
• Locus ID: 27250
• Domains: MA3
• Protein Families: Druggable Genome
• Gene Summary: This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (2) includes an alternate exon in the 5' coding region and utilizes a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus, compared to isoform 1.