HNF6 (ONECUT1) (NM_004498) Human Untagged Clone

CAT#: SC313601

ONECUT1 (untagged)-Human one cut homeobox 1 (ONECUT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ONECUT1
• Synonyms: HNF-6; HNF6; HNF6A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004498 edited
  ATGAACGCGCAGCTGACCATGGAAGCGATCGGCGAGCTGCACGGGGTGAGCCATGAGCCG
  GTGCCCGCCCCTGCCGACCTGCTGGGCGGCAGCCCCCACGCGCGCAGCTCCGTGGCGCAC
  CGCGGCAGCCACCTGCCCCCCGCGCACCCGCGCTCCATGGGCATGGCGTCCCTGCTGGAC
  GGCGGCAGCGGCGGCGGAGATTACCACCACCACCACCGGGCCCCTGAGCACAGCCTGGCC
  GGCCCCCTGCATCCCACCATGACCATGGCCTGCGAGACTCCCCCAGGTATGAGCATGCCC
  ACCACCTACACCACCTTGACCCCTCTGCAGCCGCTGCCTCCCATCTCCACAGTCTCGGAC
  AAGTTCCCCCACCATCACCACCACCACCATCACCACCACCACCCGCACCACCACCAGCGC
  CTGGCGGGCAACGTGAGCGGTAGCTTCACGCTCATGCGGGATGAGCGCGGGCTGGCCTCC
  ATGAATAACCTCTATACCCCCTACCACAAGGACGTGGCCGGCATGGGCCAGAGCCTCTCG
  CCCCTCTCCAGCTCCGGTCTGGGCAGCATCCACAACTCCCAGCAAGGGCTCCCCCACTAT
  GCCCACCCGGGGGCCGCCATGCCCACCGACAAGATGCTCACCCCCAACGGCTTCGAAGCC
  CACCACCCGGCCATGCTCGGCCGCCACGGGGAGCAGCACCTCACGCCCACCTCGGCCGGC
  ATGGTGCCCATCAACGGCCTTCCTCCGCACCATCCCCACGCCCACCTGAACGCCCAGGGC
  CACGGGCAACTCCTGGGCACAGCCCGGGAGCCCAACCCTTCGGTGACCGGCGCGCAGGTC
  AGCAATGGAAGTAATTCAGGTCAGATGGAAGAGATCAATACCAAAGAGGTGGCGCAGCGT
  ATCACCACCGAGCTCAAGCGCTACAGCATCCCACAGGCCATCTTCGCGCAGAGGGTGCTC
  TGCCGCTCCCAGGGGACCCTCTCGGACCTGCTGCGCAACCCCAAACCCTGGAGCAAACTC
  AAATCCGGCCGGGAGACCTTCCGGAGGATGTGGAAGTGGCTGCAGGAGCCGGAGTTCCAG
  CGCATGTCCGCGCTCCGCTTAGCAGCATGCAAAAGGAAAGAACAAGAACATGGGAAGGAT
  AGAGGCAACACACCCAAAAAGCCCAGGTTGGTCTTCACAGATGTCCAGCGTCGAACTCTA
  CATGCAATATTCAAGGAAAATAAGCGTCCATCCAAAGAATTGCAAATCACCATTTCCCAG
  CAGCTGGGGTTGGAGCTGAGCACTGTCAGCAACTTCTTCATGAACGCAAGAAGGAGGAGT
  CTGGACAAGTGGCAGGACGAGGGCAGCTCCAATTCAGGCAACTCATCTTCTTCATCAAGC
  ACTTGTACCAAAGCATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004498
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004498.1, NP_004489.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 1398 bp
• Locus ID: 3175
• Cytogenetics: 15q21.3
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.