SESN3 (NM_144665) Human Untagged Clone

CAT#: SC313706

SESN3 (untagged)-Human sestrin 3 (SESN3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SESN3
• Synonyms: SEST3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_144665 edited
  CCTAGCCTGGCGGGGGCGGGGGTCTGGAAGAGGGGGCGGGTGGAACAACGGGCCGGTGAG
  GAGCAGCCGCCGCCGTCTCCGCCGTCGCTGGGGCAGCTGCCGCGGTGGTCGCCTCTGGCA
  GTGCCGCTAGCTTTCAGGCAGTCGCCTCTCCTCCCGACATCCCGCCTTGAGGAGGCTGAG
  GCGGAATCGCGGCTGGCGGCGGGGGGACGGACCGGCCCTGCAGTGGCCGCAGTGACAGCC
  CGGACCCGGCCCTCCGCCCGCTCTCGCCTCGGCGCCCGCAGCCACGATGAACCGGGGCGG
  CGGCAGCCCGTCGGCCGCCGCCAACTACCTGCTCTGTACCAACTGCCGGAAAGTGCTGCG
  GAAGGATAAAAGAATCAGAGTGTCTCAACCCTTGACAAGAGGACCAAGTGCCTTTATTCC
  AGAGAAGGAAGTTGTCCAAGCAAACACAGTGGATGAACGTACTAACTTTCTTGTGGAAGA
  ATACTCTACATCCGGTCGTCTGGACAACATCACACAGGTCATGAGTTTACACACTCAGTA
  CCTGGAGTCTTTCTTGCGGAGCCAGTTTTACATGTTGCGCATGGATGGTCCCCTTCCTCT
  ACCATACAGGCACTATATTGCAATAATGGCTGCAGCTAGACATCAGTGTTCTTACTTAAT
  AAACATGCATGTGGATGAATTTTTAAAGACTGGAGGTATTGCTGAGTGGTTGAATGGTTT
  GGAATATGTGCCACAAAGACTGAAAAATCTTAATGAAATTAATAAGCTGCTAGCACATCG
  ACCTTGGCTGATCACAAAAGAGCACATTCAGAAACTTGTCAAAACTGGAGAAAATAATTG
  GTCTCTGCCTGAACTGGTACATGCTGTGGTCCTCCTGGCACATTATCATGCTTTGGCAAG
  CTTTGTTTTTGGTAGTGGTATCAATCCAGAGAGAGATCCAGAAATCTCCAATGGATTCAG
  GCTAATATCAGTCAACAATTTCTGCGTTTGTGATCTTGCTAATGACAACAACATAGAGAA
  TGCATCTCTTTCAGGCAGCAACTTTGGGATTGTGGATTCTCTAAGTGAGCTAGAGGCCTT
  AATGGAAAGGATGAAAAGACTTCAAGAAGAAAGGGAAGATGAAGAGGCGTCTCAAGAAGA
  AATGAGCACTCGTTTTGAAAAGGAGAAGAAAGAAAGTCTTTTTGTGGTCTCTGGAGATAC
  TTTTCATTCATTTCCTCATTCAGATTTTGAGGATGACATGATTATAACATCTGATGTCTC
  TCGATATATTGAAGACCCTGGTTTTGGGTATGAAGACTTTGCCAGACGAGGAGAAGAGCA
  TTTGCCAACATTCCGAGCTCAGGACTATACCTGGGAAAATCATGGGTTCTCCCTGGTGAA
  CAGACTTTATTCTGACATTGGACATCTTCTTGATGAAAAGTTTCGGATGGTCTACAATCT
  CACATATAACACTATGGCCACCCATGAGGATGTTGACACAACCATGCTGCGCAGAGCTTT
  ATTTAACTATGTTCACTGTATGTTTGGAATCAGGTATGATGACTATGATTATGGAGAAGT
  TAATCAATTACTTGAAAGAAGCCTGAAGGTTTACATTAAGACAGTGACCTGCTATCCTGA
  GAGAACTACAAAACGCATGTATGATAGTTACTGGCGGCAGTTCAAACACTCAGAAAAAGT
  TCATGTCAATCTACTTTTAATGGAAGCACGAATGCAAGCTGAACTTCTTTATGCTCTTCG
  TGCCATAACTCGGCATTTGACCTGAAGTATCACCCAAGGAAAATGTCATACATGTGTAAA
  GACCTTTCACATAAGATAAACATTGAAGCTATTTGTGATATAGCATCAAAAATTATTCAG
  TTCTCTAGTGTCAAAGTTTAGCCGTTTGTTTTTTTTTKTTTTTTGTTTTTTGCGGCTGTA
  ATGTGCAATGATGTGTTTTATTTTCCTTGATGCTTAACATTACTAACAATTGCAAAAATA
  ATACTGAGGAGCACTACTTTGCATTGTTTGTAGTTGGAGTTTTGGATACTGATCATAAAT
  CATGAATCTGGCGTATTAATGCTTAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144665 unedited
  CCGCGGGACCNCCNCCCCTTTCNCCCCCCGGTTCAGATTTGTATACGACTCACTTATAGG
  CGGCCGCGNATTCGGCACGAGCCTAGCCTGGCGGGGGCGGGGGTCTGGAAGAGGGGGCGG
  GTGGAACAACGGGCCGGTGAGGAGCAGCCGCCGCCGTCTCCGCCGTCGCTGGGGCAGCTG
  CCGCGGTGGTCGCCTCTGGCAGTGCCGCTAGCTTTCAGGCAGTCGCCTCTCCTCCCGACA
  TCCCGCCTTGAGGAGGCTGAGGCGGAATCGCGGCTGGCGGCGGGGGGACGGACCGGCCCT
  GCAGTGGCCGCAGTGACAGCCCGGACCCGGCCCTCCGCCCGCTCTCGCCTCGGCGCCCGC
  AGCCACGATGAACCGGGGCGGCGGCAGCCCGTCGGCCGCCGCCAACTACCTGCTCTGTAC
  CAACTGCCGGAAAGTGCTGCGGAAGGATAAAAGAATCAGAGTGTCTCAACCCTTGACAAG
  AGGACCAAGTGCCTTTATTCCAGAGAAGGAAGTTGTCCAAGCAAACACAGTGGATGAACG
  TACTAACTTTCTTGTGGAAGAATACTCTACATCCGGTCGTCTGGGACACATCACACAGGT
  CATGANGTTACACACTCAGTACCTGGAGTCTTTCTTGCGGAGCCAGTTTTACATGTTGCG
  CATGGATGGTCCCCTTCCTCTACCATACAGGCACTATATTGCAATAATGGCTGCAGCTAG
  ACATCAGTGTTCTTTACCTTATAACATGCATGTGGATGAATTTTTAAAGACTGGAGGTAT
  TGCTGAATGGTGAATGGTTTGGAATTTTGCCCCAAGACTGAAAATCTTATGAATTAATAG
  CTGTTACCCTTAACCTTGCTGATCCAAAGAACCTTCCTGACTTGTAAAACTGGAAAAAAA
  TTGGCTTTGCTGACTGGACATGTTGGGCCCTCCTGCCCTTAAATGCTTTGAAACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_144665 unedited
  GANGAGCCTGGGGNGGGGTCACAGGNATGCCACCCGGGTTCTGTTCAGGAAAAGCTATGA
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGCATTAA
  TACGCCAGATTCATGATTTATGATCAGTATCCAAAACTCCAACTACAAACAATGCAAAGT
  AGTGCTCCTCAGTATTATTTTTGCAATTGTTAGTAATGTTAAGCATCAAGGAAAATAAAA
  CACATCATTGCACATTACAGCCGCAAAAAACAAAAAACAAAAAAAAACAAACGGCTAAAC
  TTTGACACTAGAGAACTGAATAATTTTTGATGCTATATCACAAATAGCTTCAATGTTTAT
  CTTATGTGAAAGGTCTTTACACATGTATGACATTTTCCTTGGGTGATACTTCAGGTCAAA
  TGCCGAGTTATGGCACGAAGAGCATAAAGAAGTTCAGCTTGCATTCGTGCTTCCATTAAA
  AGTAGATTGACATGAACTTTTTCTGAGTGTTTGAACTGCCGCCAGTAACTATCATACATG
  CGTTTTGTAGTTCTCTCAGGATAGCAGGTCACTGTCTTAATGTAAACCTTCAGGCTTCTT
  TCAAGTAATTGATTAACTTCTCCATAATCATAGTCATCATACCTGATTCCAAACATACAG
  TGAACATAGTTAAATAAAGCTCTGCGCAGCATGGTTGTGTCAACATCCTCATGGGTGGCC
  ATAGTGTTATATGTGAGATTGTAGACCATCCGAAACTTTTCATCAAGAAGATGTCCAATG
  TCAGAATAAAGTCTGTTCACCAGGGGAGAACCCATGATTTTCCCAGGTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_144665
• ORF Size: 1479 bp
• Insert Size: 2100
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_144665.2, NP_653266.2
• RefSeq Size: 1987
• RefSeq ORF: 1479
• Locus ID: 143686
• Domains: PA26
• Protein Pathways: p53 signaling pathway
• Gene Summary: This gene encodes a member of the sestrin family of stress-induced proteins. The encoded protein reduces the levels of intracellular reactive oxygen species induced by activated Ras downstream of RAC-alpha serine/threonine-protein kinase (Akt) and FoxO transcription factor. The protein is required for normal regulation of blood glucose, insulin resistance and plays a role in lipid storage in obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.