Caldesmon (CALD1) (NM_033140) Human Untagged Clone

CAT#: SC313891

CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALD1
• Synonyms: CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033140, the custom clone sequence may differ by one or more nucleotides
  ATGCTGGGTGGATCCGGATCGCATGGAAGACGCAGCCTGGCCGCGCTCTCCCAAATCGCC
  TACCAGAGGAATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGA
  CAGGAACGGCTGCGGCAGAAGCAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTG
  GAGGTGAATGCCCAGAACAGTGTGCCTGACGAGGAGGCCAAGACAACCACCACAAACACT
  CAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCCTGGCTCGGCGTGAGGAAAGA
  CGCCAAAAACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACCCAACAATAACA
  GATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGACACAGCAGAAAATGAAACT
  ACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAGAGGAAACAGAA
  ACAGTCACCAAGTCCTACCAGAAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAA
  GACAAGGAAAAGGAGGAGGAGGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAAT
  CAGATCAAAGATGAAAAGATTAAAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTC
  ATGGATCGAAAGAAGGGATTTACAGAAGTTAAGTCGCAGAATGGAGAATTCATGACCCAC
  AAACTTAAACATACTGAGAATACTTTCAGCCGCCCTGGAGGGAGGGCCAGCGTGGACACC
  AAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCGGCAAAAGGCTGGAGGAGCTTCGTCGT
  CGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAACAGAAGCAGCAGGAGGCG
  GCTTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTGGAGGAG
  GAAGAGCAGAGGAGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAG
  AGGAGGCTAAAGGAAGAGATTGAAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAG
  ATGCCAGAAGATGGCTTGTCAGATGACAAGAAACCATTCAAGTGTTTCACTCCTAAAGGT
  TCATCTCTCAAGATAGAAGAGCGAGCAGAATTTTTGAATAAGTCTGTGCAGAAAAGCAGT
  GGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCAAGATTGACAGCAGACTGGAGCAG
  TATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACAAAGCCGGCAGCCTCGGAT
  CTTCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAAAGGGAATGTG
  TTTTCATCCCCCACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGG
  GTTTCTAGCCGCATCAATGAATGGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCT
  CCCAAACCTTCTGACTTGAGACCAGGAGACGTATCCAGCAAGCGGAACCTCTGGGAAAAG
  CAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTT
  
• Restriction Sites: Please inquire
• ACCN: NM_033140
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033140.2, NP_149131.1
• RefSeq Size: 4204 bp
• RefSeq ORF: 1599 bp
• Locus ID: 800
• Cytogenetics: 7q33
• Domains: Caldesmon
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1. It is mainly expressed in non-muscle tissues or cells. The resulting isoform (5, also known as HeLa l-CaD II) has a distinct N-terminus and is shorter than isoform 1.