MAP4 (AK054696) Human Untagged Clone

CAT#: SC313916

(untagged)-Human cDNA FLJ30134 fis, clone BRACE1000187, weakly similar to MICROTUBULE-ASSOCIATED PROTEIN 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK054696, the custom clone sequence may differ by one or more nucleotides
  ATGTCGCTCTCAGACAAGCAGACAGCCTCTCTCACTGCCGCGTACGGTCAGCTCAGTAAG
  GGCAAGCCTGCAGAGTGCCGAATGGACTCCCCAAAAGAAATCAGTCAAGCCGGATTCGAA
  TGGCAGAGGACAGAGGGCAAACTGAATGAAATTGGGCTGAATGTCAGCATGGACGGGCAA
  CCAAAAGATGGGCTTGTGAAGAATGCCAGCTTCCTGGAGCAGAACAAGCTCTGCTTTTTT
  GAGGGGAAGCTAGACAAAGAGCTGAGCATTGAAATGCAGGACAAGGACTGTCAAGAAGCC
  TCAGGTCACCTTGAGAGCAGGTATGTGATTTCAGAGACCTGCCATCCCTTGGAGGGGAAC
  TCGGTACACCAGAAGACCTCCGAGTTCCATCTGGGACTCATAGAGGGGCCAGACAAAAAC
  AAAACCATTCCAGTTCAGGGGAAGGTGGCAGGGAAGAATGGACTAGAGACCAAGAGCCAG
  TCAGATCTGGATTTCCCTGGGGCTGCTGACATCCCTACCAGATATGTTAAGGAGCAGGAA
  ACCAGTGTTTGGAACCCCAGCTTTCATCCAGTGGCTCAAGGCTCTCTGGGCTCAAGGGAA
  GCAACTCCGGGAGAGATGGAGAATAGCATCACCCCTGGCTGCCCAGTGATTGGGGTGGTA
  AATGATAACTCTGAGCAGCTGAAGTGTGAGTCCCCACTCCTGGTGTCTCTAGCCCACCCA
  GCCCCCATTATTGAGCATTCACCCACCACCATTCCGCCAATCACTATGGTGTTCACCCAG
  GAACATTTGAATGCAAGCTGTCACATCAGAGACCATGATAAGGAGTTGGAGAAATTGAGT
  TCTACCGAGGAGGCTGTGCTCAACCAAGCCCCCCAGCAGAAAAAGGCAGTGCGCAGGGCC
  CTGTCTGAATGTTCTCACCTCTCAGTTCCCCCAGCTGTCAACCTTGCAGATAAGTACCCT
  GAACTCCCTGCCCGAGAAGAGCCTTCTTCTGGCCTGCTGCCTCCCCCTAGTAGCCCAATG
  CCTAGTCCTACACCTGGGAAACTGGGAGCTCCTGCTATGAAGCGCTCCATGACTGTGGGT
  GAGGAACAGACAGCTAGCTACAAATTGAGCCCTGGGAAACTGCCCATCTTGTCTACTAAA
  GAGATACCTCCTTTCATCTGTGAGGAACCAGTGGCCAAGAAGAGAGAAGAATTGGCTCAC
  TTCAGCAACAGCAGCAGCAACTCTGGGAAGAAGGAACTCGGCACTGCTGGATTATATCTC
  CATAGTAAGCTGGAGCAGATTCCTGAAGGAAGCAGCAAGGAAAAAGGGCAGGAAGATTTT
  AGTGAAACTAGAATTGATTCATGCTCGCAGGTTTGCCAGCGAGGAGAGAAACAGCCAGGA
  CAGACGGCTCTGGCAGGGAAGAAAGAAATTGAGGTCACTGCAACCCAGAGCACTCCATCG
  TTCCTGTTTGAAAAGCCCCCACGTGATGGAATAGCCAGGCCAGAAGAAGGAAGGCCTGTG
  GTGAGTGGGACAGGAAATGACATCACCACCCCACCGAACAAGGAGCTCCCACCAAGCCCA
  GAGAAGAAAACAAAGGTAGGTGCCAGAATGGTGGTTATCTTCTACTGCCATAATTTT
  
• Restriction Sites: Please inquire
• ACCN: AK054696
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK054696.1, BAB70795.1
• RefSeq Size: 2156 bp
• RefSeq ORF: 1620 bp
• Locus ID: 4134
• Cytogenetics: 3p21.31
• Gene Summary: 'The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'