NF-kB p65 (RELA) (NM_021975) Human Untagged Clone

CAT#: SC313967

RELA (untagged)-Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RELA
• Synonyms: CMCU; NFKB3; p65
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021975 edited
  GAATTCGGCACGAGGCGCCCCCGGGACCCCGGCCATGGACGAACTGTTCCCCCTCATCTT
  CCCGGCAGAGCCAGCCCAGGCCTCTGGCCCCTATGTGGAGATCATTGAGCAGCCCAAGCA
  GCGGGGCATGCGCTTCCGCTACAAGTGCGAGGGGCGCTCCGCGGGCAGCATCCCAGGCGA
  GAGGAGCACAGATACCACCAAGACCCACCCCACCATCAAGATCAATGGCTACACAGGACC
  AGGGACAGTGCGCATCTCCCTGGTCACCAAGGACCCTCCTCACCGGCCTCACCCCCACGA
  GCTTGTAGGAAAGGACTGCCGGGATGGCTTCTATGAGGCTGAGCTCTGCCCGGACCGCTG
  CATCCACAGTTTCCAGAACCTGGGAATCCAGTGTGTGAAGAAGCGGGACCTGGAGCAGGC
  TATCAGTCAGCGCATCCAGACCAACAACAACCCCTTCCAAGTTCCTATAGAAGAGCAGCG
  TGGGGACTACGACCTGAATGCTGTGCGGCTCTGCTTCCAGGTGACAGTGCGGGACCCATC
  AGGCAGGCCCCTCCGCCTGCCGCCTGTCCTTTCTCATCCCATCTTTGACAATCGTGCCCC
  CAACACTGCCGAGCTCAAGATCTGCCGAGTGAACCGAAACTCTGGCAGCTGCCTCGGTGG
  GGATGAGATCTTCCTACTGTGTGACAAGGTGCAGAAAGAGGACATTGAGGTGTATTTCAC
  GGGACCAGGCTGGGAGGCCCGAGGCTCCTTTTCGCAAGCTGATGTGCACCGACAAGTGGC
  CATTGTGTTCCGGACCCCTCCCTACGCAGACCCCAGCCTGCAGGCTCCTGTGCGTGTCTC
  CATGCAGCTGCGGCGGCCTTCCGACCGGGAGCTCAGTGAGCCCATGGAATTCCAGTACCT
  GCCAGATACAGACGATCGTCACCGGATTGAGGAGAAACGTAAAAGGACATATGAGACCTT
  CAAGAGCATCATGAAGAAGAGTCCTTTCAGCGGACCCACCGACCCCCGGCCTCCACCTCG
  ACGCATTGCTGTGCCTTCCCGCAGCTCAGCTTCTGTCCCCAAGCCAGCACCCCAGCCCTA
  TCCCTTTACGTCATCCCTGAGCACCATCAACTATGATGAGTTTCCCACCATGGTGTTTCC
  TTCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCCCGGCCCCTCCCCAAGTCCTGCCCCA
  GGCTCCAGCCCCTGCCCCTGCTCCAGCCATGGTATCAGCTCTGGCCCAGGCCCCAGCCCC
  TGTCCCAGTCCTAGCCCCAGGCCCTCCTCAGGCTGTGGCCCCACCTGCCCCCAAGCCCAC
  CCAGGCTGGGGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCTGCAGTTTGATGATGAAGA
  CCTGGGGGCCTTGCTTGGCAACAGCACAGACCCAGCTGTGTTCACAGACCTGGCATCCGT
  CGACAACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCATACCTGTGGCCCCCCACACAAC
  TGAGCCCATGCTGATGGAGTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGCCCAGAG
  GCCCCCCGACCCAGCTCCTGCTCCACTGGGGGCCCCGGGGCTCCCCAATGGCCTCCTTTC
  AGGAGATGAAGACTTCTCCTCCATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGAT
  CAGCTCCTAAGGGGGTGACGCCTGCCCTCCCCAGAGCACTGGGTTGCAGGGGATTGAAGC
  CCTCCAAAAGCACTTACGGATTCTGGTGGGGTGTGTTCCAACTGCCCCCAACTTTGTGGA
  TGTCTTCCTTGGAGGGGGGAGCCATATTTTATTCTTTTATTGTCAGTATCTGTATCTCTC
  TCTCTTTTTGGAGGTGCTTAAGCAGAAGCATTAACTTCTCTGGAAAGGGGGGAGCTGGGG
  AAACTCAAACTTTTCCCCTGTCCTGATGGTCAGCTCCCTTCTCTGTAGGGAACTCTGGGG
  TCCCCCATCCCCATCCTCCAGCTTCTGGTACTCTCCTAGAGACAGAAGCAGGCTGGAGGT
  AAGGCCTTTGAGCCCACAAAGCCTTATCAAGTGTCTTCCATCATGGATTCATTACAGCTT
  AATCAAAATAACGCCCCAGATACCAGCCCCTGTATGGCACTGGCATTGTCCCTGTGCCTA
  ACACCAGCGTTTGAGGGGCTGGCCTTCCTGCCCTACAGAGGTCTCTGCCGGCTCTTTCCT
  TGCTCAACCATGGCTGAAGGAAACCAGTGCAACAGCACTGGCTCTCTCCAGGATCCAGAA
  GGGGTTTGGTCTGGGACTTCCTTGCTCTCCCTCTTCTCAAGTGCCTTAATAGTAGGGTAA
  GTTGTTAAGAGTGGGGGAGAGCAGGCTGGCAGCTCTCCAGTCAGGAGGCATAGTTTTTAC
  TGAACAATCAAAGCACTTGGACTCTTGCTCTTTCTACTCTGAACTAATAAATCTGTTGCC
  AAGCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021975 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCCCCGGGACCCCGGCCA
  TGGACGAACTGTTCCCCCTCATCTTCCCGGCAGAGCCAGCCCAGGCCTCTGGCCCCTATG
  TGGAGATCATTGAGCAGCCCAAGCAGCGGGGCATGCGCTTCCGCTACAAGTGCGAGGGGC
  GCTCCGCGGGCAGCATCCCAGGCGAGAGGAGCACAGATACCACCAAGACCCACCCCACCA
  TCAAGATCAATGGCTACACAGGACCAGGGACAGTGCGCATCTCCCTGGTCACCAAGGACC
  CTCCTCACCGGCCTCACCCCCACGAGCTTGTAGGAAAGGACTGCCGGGATGGCTTCTATG
  AGGCTGAGCTCTGCCCGGACCGCTGCATCCACAGTTTCCAGAACCTGGGAATCCAGTGTG
  TGAAGAAGCGGGACCTGGAGCAGGCTATCAGTCAGCGCATCCAGACCAACAACAACCCCT
  TCCAAGTTCCTATAGAAGAGCAGCGTGGGGACTACGACCTGAATGCTGTGCGGCTCTGCT
  TCCAGGTGACAGTGCGGGACCCATCAGGCAGGCCCCTCCGCCTGCCGCCTGTCCTTTCTC
  ATCCCATCTTTGACAATCGTGCCCCCAACACTGCCGAGCTCAAGATCTGCCGAGTGAACC
  GAAACTCTGGCAGCTGCCTCGGTGGAGATGAGATCTTCCTACTGTGTGACAAGGTGCAGA
  AAGAGGACATTGANGGTGTATTTTACGGGACCAGGCTGGGAGGCCCGAGGCTNCTTTTCG
  CAAGCTGATGTGCACCGACAGTGGCCATTGTGTTNCGGACCCCTCCTACGCAACCCCANN
  CTGCAGCTCTGTGCGTGTCTCATGCAGCTGCGGCGGCCTTCGACCGGAGCTCATGAGCCA
  TGGATTNCAGTACTGGCAGATCAAGAGATCGTNCCGGGATGAGGAGAACGTAAAGGACTA
  TGAGACCTCAGAGCTCATGAAAAAAGTCTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_021975 unedited
  NTTTTTGGCACGCTATATTATTCAGATANAAGNACAGNATCCAGTGCTTTGATTGTTCAG
  TAAAAACTATGCCTCCTGACTGGAGAGCTGCCAGCCTGCTCTCCCCCACTCTTAACAACT
  TACCCTACTATTAAGGCACTTGAGAAGAGGGAGAGCAAGGAAGTCCCAGACCAAACCCCT
  TCTGGATCCTGGAGAGAGCCAGTGCTGTTGCACTGGTTTCCTTCAGCCATGGTTGAGCAA
  GGAAAGAGCCGGCAGAGACCTCTGTAGGGCAGGAAGGCCAGCCCCTCAAACGCTGGTGTT
  AGGCACAGGGACAATGCCAGTGCCATACAGGGGCTGGTATCTGGGGCGTTATTTTGATTA
  AGCTGTAATGAATCCATGATGGAAGACACTTGATAAGGCTTTGTGGGCTCAAAGGCCTTA
  CCTCCAGCCTGCTTCTGTCTCTAGGAGAGTACCAGAAGCTGGAGGATGGGGATGGGGGAC
  CCCAGAGTTCCCTACAGAGAAGGGAGCTGACCATCAGGACAGGGGAAAAGTTTGAGTTTC
  CCCAGCTCCCCCCTTTCCAGAGAAGTTAATGCTTCTGCTTAAGCACCTCCAAAAAGAGAG
  AGAGATACAGATACTGACAATAAAAGAATAAAATATGGCTCCCCCCTCCAAGGAAGACAT
  CCACAAAGTTGGGGGCAGTTGGAACACACCCCACCAGAATCCGTAAGTGCTTTTGGAGGG
  CTTCAATCCCCTGCAACCCAGTGCTCTGGGGAGGGCAGGCGTCACCCNNCTTAGAGCTGA
  TCTGACTCAGCAGGGCTGAGAAGTCCATGTCCGCAATGGAGGAGAAGTCTTCATCTNCTG
  AAAGGNAGCCATTGNNGAGCCCCGGNGCCCNCAGTGGNAGCAGAGCTGGGGTCGGGGGGC
  CTCTGGGGCCCTGTCACTANGCGAGTNTAGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021975
• Insert Size: 2620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the sub of 1 aa.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021975.2, NP_068810.2
• RefSeq Size: 1760 bp
• RefSeq ORF: 1656 bp
• Locus ID: 5970
• Cytogenetics: 11q13.1
• Domains: RHD, IPT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: 'NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1).