MBD1 (NM_015845) Human Untagged Clone

CAT#: SC314111

MBD1 (untagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBD1
• Synonyms: CXXC3; PCM1; RFT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015845, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGGACTGGCTGGACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGT
  CAGGGGCCACCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAGT
  TGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAGGCATCTTGTGC
  TATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAAAGAAGCCTTCAAGGCCAGCCA
  AGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGGTCAGGAAGGAGGCCCCGAGGGATGAGACCAA
  GGCTGACACTGACACAGCCCCAGCTTCATTCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTC
  TCAGGGGATGGCACCCAAAGGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCT
  TCAACCGGGAACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAGA
  CTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGTTCTGCAAGTGT
  GAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAGTATGCCGGGGCTGTCAGACCC
  AAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTCCCCGCCCTGGTCTCAGGCGCCAGTGGAAATG
  TGTCCAGCGACGTTGCCTACGGGGTAAACATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGGCTGCC
  AGGCGGCGCCCCGGAGCCCAGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGAGCCGC
  AGCCCTACACGAACCGCCGGCAGAACCGCAAGTGCGGGGCCTGTGCAGCCTGCCTACGGCGGATGGACTG
  TGGCCGCTGCGACTTCTGCTGCGACAAGCCCAAATTCGGGGGCAGCAACCAGAAGCGCCAGAAGTGTCGT
  TGGCGCCAATGCCTGCAGTTTGCCATGAAGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGG
  CAGGATCGCCCCCACCTTACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTAC
  CTTGAAGCCCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAGCA
  GGTGGTGGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGGGAAGGCGCAAGCAGTCCTG
  TGCAGGTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTGTTGCAGGCAGTAGACCCAGGCCTGCCTTC
  TGTGAAGCAAGAGCCACCTGACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTG
  GCCCCAGAGGAAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCC
  GCTTCCGAGATACAGCAGTCTGGTTGCCAAGTCTGCAGGGCAGGCACTCGGGAAGGGAAGATGGATGTAA
  AGTGTGGGAGACCGAGGACACAGTGGAGCCCACGAGCACGAGCTGGAACCCACGAGGATGGCCTGGAACC
  CATGTCAGTCTCTCACCACCTCCAGCTTCGATGATGTGGGTGTCCTGCAGAAGAAGCTGGTGCCCTTCCT
  CACAGAGTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015845
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015845.3, NP_056670.2
• RefSeq Size: 3005 bp
• RefSeq ORF: 1761 bp
• Locus ID: 4152
• Cytogenetics: 18q21.1
• Domains: MBD, zf-CXXC
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) is missing 3 non-consecutive coding exons compared to variant 1, the last of which causes a frame-shift. This results in a shorter isoform (2) with a distinct C-terminus compared to isoform 1.