GRB10 (AJ271366) Human Untagged Clone

CAT#: SC314132

(untagged)-Human mRNA for putative adaptor protein (GRB10 gene)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRB10
• Synonyms: GRB-IR|Grb-10|IRBP|MEG1|RSS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AJ271366, the custom clone sequence may differ by one or more nucleotides
  ATGGCTTTAGCCGGCTGCCCAGATTCCTTTTTGCACCATCCGTACTACCAGGACAAGGTG
  GAGCAGACACCTCGCAGTCAACAAGACCCGGCAGGACCAGGACTCCCCGCACAGTCTGAC
  CGACTTGCGAATCACCAGGAGGATGATGTGGACCTGGAAGCCCTGGTGAACGATATGAAT
  GCATCCCTGGAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGACACGGTGCCCCTCCTG
  CAGAATGGCCAGCATGCCCGCAGCCAGCCTCGGGCTTCAGGCCCTCCTCGGTCCATCCAG
  CCACAGGTGTCCCCGAGGCAGAGGGTGCAGCGCTCCCAGCCTGTGCACATCCTCGCTGTC
  AGGCGCCTTCAGGAGGAAGACCAGCAGTTTAGAACCTCATCTCTGCCGGCCATCCCCAAT
  CCTTTTCCTGAACTCTGTGGCCCTGGGAGCCCCCCTGTGCTCACGCCGGGTTCTTTACCT
  CCGAGCCAGGCCGCCGCAAAGCAGGATGTTAAAGTCTTTAGTGAAGATGGGACAAGCAAA
  GTGGTGGAGATTCTAGCAGACATGACAGCCAGAGACCTGTGCCAATTGCTGGTTTACAAA
  AGTCACTGTGTGGATGACAACAGCTGGACACTAGTGGAGCACCACCCGCACCTAGGATTA
  GAGAGGTGCTTGGAAGACCATGAGCTGGTGGTCCAGGTGGAGAGTACCATGGCCAGTGAG
  AGTAAATTTCTATTCAGGAAGAATTACGCAAAATACGAGTTCTTTAAAAATCCCATGAAT
  TTCTTCCCAGAACAGATGGTTACTTGGTGCCAGCAGTCAAATGGCAGTCAAACCCAGCTT
  TTGCAGAATTTTCTGAACTCCAGTAGTTGTCCTGAAATTCAAGGGTTTTTGCATGTGAAA
  GAGCTGGGAAAGAAATCATGGAAAAAGCTGTATGTGTGTTTGCGGAGATCTGGCCTTTAT
  TGCTCCACCAAGGGAACTTCAAAGGAACCCAGACACCTGCAGCTGCTGGCCGACCTGGAG
  GACAGCAACATCTTCTCCCTGATCGCTGGCAGGAAGCAGTACAACGCCCCTACAGACCAC
  GGGCTCTGCATAAAGCCAAACAAAGTCAGGAATGAAACTAAAGAGCTGAGGTTGCTCTGT
  GCAGAGGACGAGCAAACCAGGACGTGCTGGATGACAGCGTTCAGACTCCTCAAGTATGGA
  ATGCTCCTTTACCAGAATTACCGAATCCCTCAGCAGAGGAAGGCCTTGCTGTCCCCGTTC
  TCGACGCCAGTGCGCAGTGTCTCCGAGAACTCCCTCGTGGCAATGGATTTTTCTGGGCAA
  ACAGGACGCGTGATAGAGAATCCGGCGGAGGCCCAGAGCGCAGCCCTGGAGGAGGGCCAC
  GCCTGGAGGAAGCGAAGCACACGGATGAACATCCTAGGTAGCCAAAGTCCCCTCCACCCT
  TCTACCCTAAGTACAGTGATTCACAGGACACAGCACTGGTTTCACGGGAGGATCTCCAGG
  GAGGAATCCCACAGGATCATTAAACAGCAAGGGCTCGTGGATGGGCTTTTTCTCCTCCGT
  GACAGCCAGAGTAATCCAAAGGCATTTGTACTCACACTGTGTCATCACCAGAAAATTAAA
  AATTTCCAGATCTTACCTTGCGAGGACGACGGGCAGACGTTCTTCAGCCTAGATGACGGG
  AACACCAAATTCTCTGACCTGATCCAGCTGGTTGACTTTTACCAGCTGAACAAAGGAGTC
  CTGCCTTGCAAACTCAAGCACCACTGCATCCGAGTGGCCTTA
  
• Restriction Sites: Please inquire
• ACCN: AJ271366
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AJ271366.1, CAB96542.1
• RefSeq Size: 2629 bp
• RefSeq ORF: 1785 bp
• Locus ID: 2887
• Cytogenetics: 7p12.1
• Domains: RA, SH2, PH
• Protein Families: Druggable Genome
• Gene Summary: 'The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]'