RASGRP2 (NM_153819) Human Untagged Clone

CAT#: SC314188

RASGRP2 (untagged)-Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRP2
• Synonyms: CALDAG-GEFI; CDC25L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153819, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGGCACCCTGGACCTGGACAAGGGCTGCACGGTGGAGGAGCTGCTCCGCGGGTGCATCGAAGCCT
  TCGATGACTCCGGGAAGGTGCGGGACCCGCAGCTGGTGCGCATGTTCCTCATGATGCACCCCTGGTACAT
  CCCCTCCTCTCAGCTGGCGGCCAAGCTGCTCCACATCTACCAACAATCCCGGAAGGACAACTCCAATTCC
  CTGCAGGTGAAAACGTGCCACCTGGTCAGGTACTGGATCTCCGCCTTCCCAGCGGAGTTTGACTTGAACC
  CGGAGTTGGCTGAGCAGATCAAGGAGCTGAAGGCTCTGCTAGACCAAGAAGGGAACCGACGGCACAGCAG
  CCTAATCGACATAGACAGCGTCCCTACCTACAAGTGGAAGCGGCAGGTGACTCAGCGGAACCCTGTGGGA
  CAGAAAAAGCGCAAGATGTCCCTGTTGTTTGACCACCTGGAGCCCATGGAGCTGGCGGAGCATCTCACCT
  ACTTGGAGTATCGCTCCTTCTGCAAGATCCTGTTTCAGGACTATCACAGTTTCGTGACTCATGGCTGCAC
  TGTGGACAACCCCGTCCTGGAGCGGTTCATCTCCCTCTTCAACAGCGTCTCACAGTGGGTGCAGCTCATG
  ATCCTCAGCAAACCCACAGCCCCGCAGCGGGCCCTGGTCATCACACACTTTGTCCACGTGGCGGAGAAGC
  TGCTACAGCTGCAGAACTTCAACACGCTGATGGCAGTGGTCGGGGGCCTGAGCCACAGCTCCATCTCCCG
  CCTCAAGGAGACCCACAGCCACGTTAGCCCTGAGACCATCAAGCTCTGGGAGGGTCTCACGGAACTAGTG
  ACGGCGACAGGCAACTATGGCAACTACCGGCGTCGGCTGGCAGCCTGTGTGGGCTTCCGCTTCCCGATCC
  TGGGTGTGCACCTCAAGGACCTGGTGGCCCTGCAGCTGGCACTGCCTGACTGGCTGGACCCAGCCCGGAC
  CCGGCTCAACGGGGCCAAGATGAAGCAGCTCTTTAGCATCCTGGAGGAGCTGGCCATGGTGACCAGCCTG
  CGGCCACCAGTACAGGCCAACCCCGACCTGCTGAGCCTGCTCACGGTGTCTCTGGATCAGTATCAGACGG
  AGGATGAGCTGTACCAGCTGTCCCTGCAGCGGGAGCCGCGCTCCAAGTCCTCGCCAACCAGCCCCACGAG
  TTGCACCCCACCACCCCGGCCCCCGGTACTGGAGGAGTGGACCTCGGCTGCCAAACCCAAGCTGGATCAG
  GCCCTCGTGGTGGAGCACATCGAGAAGATGGTGGAGTCTGTGTTCCGGAACTTTGACGTCGATGGGGATG
  GCCACATCTCACAGGAAGAATTCCAGATCATCCGTGGGAACTTCCCTTACCTCAGCGCCTTTGGGGACCT
  CGACCAGAACCAGGATGGCTGCATCAGCAGGGAGGAGATGGTTTCCTATTTCCTGCGCTCCAGCTCTGTG
  TTGGGGGGGCGCATGGGCTTCGTACACAACTTCCAGGAGAGCAACTCCTTGCGCCCCGTCGCCTGCCGCC
  ACTGCAAAGCCCTGATCCTGGGCATCTACAAGCAGGGCCTCAAATGCCGAGCCTGTGGAGTGAACTGCCA
  CAAGCAGTGCAAGGATCGCCTGTCAGTTGAGTGTCGGCGCAGGGCCCAGAGTGTGAGCCTGGAGGGGTCT
  GCACCCTCACCCTCACCCATGCACAGCCACCATCACCGCGCCTTCAGCTTCTCTCTGCCCCGCCCTGGCA
  GGCGAGGCTCCAGGCCTCCAGAGATCCGTGAGGAGGAGGTACAGACGGTGGAGGATGGGGTGTTTGACAT
  CCACTTGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_153819
• ORF Size: 1830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_153819.1, NP_722541.1
• RefSeq Size: 2310
• RefSeq ORF: 1830
• Locus ID: 10235
• Domains: RasGEFN, RasGEF, EFh, DAG_PE-bind
• Protein Pathways: Chemokine signaling pathway, MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longer isoform (a). Variants 2, 3, and 4 all encode the same isoform (a).