Histidine decarboxylase (HDC) (NM_002112) Human Untagged Clone

CAT#: SC314351

HDC (untagged)-Human histidine decarboxylase (HDC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HDC
• Synonyms: MGC163399
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002112, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGAGCCTGAGGAGTACAGAGAGAGAGGGAGAGAGATGGTGGATTACATCTGCCAGTACCTGAGCA
  CTGTGCGGGAGAGACGTGTGACGCCAGACGTGCAGCCTGGCTACCTGCGAGCCCAGCTGCCTGAGAGTGC
  TCCTGAGGACCCCGACAGCTGGGACAGCATCTTTGGGGACATTGAACGAATCATCATGCCTGGGGTGGTA
  CATTGGCAGAGCCCCCATATGCACGCCTACTACCCAGCCCTCACCTCTTGGCCCTCCCTGCTAGGAGACA
  TGCTGGCTGATGCCATCAACTGCTTGGGATTCACCTGGGCATCCAGCCCTGCGTGTACAGAGCTGGAGAT
  GAACGTCATGGACTGGTTGGCAAAAATGCTGGGACTTCCAGAGCACTTCTTGCACCACCACCCCAGCAGC
  CAGGGCGGAGGCGTCCTGCAGAGCACGGTCAGTGAATCCACTTTGATTGCCCTGCTGGCAGCAAGGAAGA
  ACAAAATCCTGGAAATGAAAACGTCTGAGCCCGATGCTGATGAGTCCTGCCTAAATGCCCGACTCGTGGC
  CTATGCCTCTGACCAGGCTCACTCCTCTGTGGAAAAGGCTGGTTTGATTTCCCTTGTGAAGATGAAATTT
  CTGCCTGTGGATGACAACTTCTCACTCCGAGGGGAAGCTCTTCAGAAGGCCATCGAGGAAGACAAGCAGC
  GGGGCTTGGTGCCCGTCTTTGTCTGTGCAACACTAGGGACCACTGGGGTCTGTGCATTTGACTGCCTGTC
  AGAGCTGGGCCCCATCTGTGCCCGTGAGGGGCTGTGGCTCCACATCGATGCTGCTTATGCAGGCACTGCC
  TTCCTGTGCCCCGAGTTCCGGGGGTTTCTGAAGGGGATTGAGTATGCCGACTCCTTCACCTTTAATCCTT
  CCAAGTGGATGATGGTGCATTTTGACTGTACTGGGTTCTGGGTCAAGGACAAGTACAAGCTGCAGCAGAC
  CTTCAGTGTGAATCCCATCTACCTCAGGCATGCCAACTCAGGCGTGGCCACCGACTTCATGCACTGGCAG
  ATCCCCCTGAGCCGACGGTTTCGCTCTGTTAAACTCTGGTTCGTGATTCGGTCCTTCGGGGTGAAGAATC
  TTCAAGCACATGTCAGACATGGTACTGAAATGGCTAAATATTTTGAATCTCTGGTCAGAAACGACCCTTC
  CTTTGAAATTCCTGCCAAGAGGCACCTTGGCCTGGTGGTTTTTCGTCTAAAGGGTCCTAATTGTCTCACA
  GAAAATGTGTTAAAGGAAATAGCTAAAGCTGGCCGTCTCTTCCTCATCCCGGCCACTATCCAGGACAAGT
  TAATCATCCGTTTCACTGTGACATCCCAGTTTACCACTAGGGATGACATCCTGAGAGACTGGAATCTCAT
  TCGAGATGCTGCCACTCTCATCCTGAGTCAGCACTGTACTTCCCAACCCAGCCCTCGGGTTGGGAACCTC
  ATCTCCCAAATCAGGGGTGCCAGAGCCTGGGCCTGTGGAACGTCCCTTCAGTCTGTCAGTGGGGCAGGAG
  ATGATCCAGTCCAGGCCAGGAAGATCATCAAGCAGCCTCAGCGTGTGGGAGCCGGTCCCATGAAAAGGGA
  AAATGGCCTCCATCTTGAAACCCTGCTGGACCCAGTTGATGACTGCTTTTCAGAAGAGGCCCCAGATGCC
  ACCAAGCACAAGCTGTCCTCCTTCCTGTTCAGTTACTTGTCTGTGCAGACTAAGAAGAAGACGGTGCGCT
  CCCTCAGTTGCAACAGTGTGCCAGTGAGTGCTCAGAAGCCACTGCCCACAGAGGCCTCTGTGAAGAATGG
  GGGCTCCTCCAGGGTCAGAATCTTTTCCAGGTTTCCAGAAGACATGATGATGCTGAAGAAAAGTGCCTTC
  AAAAAACTCATCAAATTCTACAGCGTCCCCAGCTTTCCTGAATGCAGCTCTCAATGTGGACTCCAGCTGC
  CCTGTTGCCCTCTGCAGGCCATGGTTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002112
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002112.3, NP_002103.2
• RefSeq Size: 2646 bp
• RefSeq ORF: 1989 bp
• Locus ID: 3067
• Cytogenetics: 15q21.2
• Domains: pyridoxal_deC
• Protein Families: Druggable Genome
• Protein Pathways: Histidine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the group II decarboxylase family and forms a homodimer that converts L-histidine to histamine in a pyridoxal phosphate dependent manner. Histamine regulates several physiologic processes, including neurotransmission, gastric acid secretion,inflamation, and smooth muscle tone.[provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).