DNA polymerase eta (POLH) (NM_006502) Human Untagged Clone

CAT#: SC314503

POLH (untagged)-Human polymerase (DNA directed), eta (POLH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLH
• Synonyms: RAD30; RAD30A; XP-V; XPV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006502, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTACTGGACAGGATCGAGTGGTTGCTCTCGTGGACATGGACTGTTTTTTTGTTCAAGTGGAGCAGC
  GGCAAAATCCTCATTTGAGGAATAAACCTTGTGCAGTTGTACAGTACAAATCATGGAAGGGTGGTGGAAT
  AATTGCAGTGAGTTATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAGAAG
  TTATGTCCAGATCTTCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAGTACCGGG
  AAGCCAGTGTTGAAGTGATGGAGATAATGTCTCGTTTTGCTGTGATTGAACGTGCCAGCATTGATGAGGC
  TTACGTAGATCTGACCAGTGCTGTACAAGAGAGACTACAAAAGCTACAAGGTCAGCCTATCTCGGCAGAC
  TTGTTGCCAAGCACTTACATTGAAGGGTTGCCCCAAGGCCCTACAACGGCAGAAGAGACTGTTCAGAAAG
  AGGGGATGCGAAAACAAGGCTTATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACCTCTCCAGA
  CCTGCAGCTCACCGTGGGAGCAGTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAGACTGGTTTT
  CAGTGTTCAGCTGGAATTTCACACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAACAAGCCCAACC
  GCCAAACCCTGGTTTCACATGGGTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGCAAAATCCGTAG
  TCTTGGAGGAAAGCTAGGGGCCTCTGTCATTGAGATCCTAGGGATAGAATACATGGGTGAACTGACCCAG
  TTCACTGAATCCCAGCTCCAGAGTCATTTTGGGGAGAAGAATGGGTCTTGGCTATATGCCATGTGCCGAG
  GGATTGAACATGATCCAGTTAAACCCAGGCAACTACCCAAAACCATTGGCTGTAGTAAGAACTTCCCAGG
  AAAAACAGCTCTTGCTACTCGGGAACAGGTACAATGGTGGCTGTTGCAATTAGCCCAGGAACTAGAGGAG
  AGACTGACTAAAGACCGAAATGATAATGACAGGGTAGCCACCCAGCTGGTTGTGAGCATTCGTGTACAAG
  GAGACAAACGCCTCAGCAGCCTGCGCCGCTGCTGTGCCCTTACCCGCTATGATGCTCACAAGATGAGCCA
  TGATGCATTTACTGTCATCAAGAACTGTAATACTTCTGGAATCCAGACAGAATGGTCTCCTCCTCTCACA
  ATGCTTTTCCTCTGTGCTACAAAATTTTCTGCCTCTGCCCCTTCATCTTCTACAGACATCACCAGCTTCT
  TGAGCAGTGACCCAAGTTCTCTGCCAAAGGTGCCAGTTACCAGCTCAGAAGCTAAGACCCAGGGAAGTGG
  CCCAGCGGTGACAGCCACTAAGAAAGCAACCACGTCTCTGGAATCATTCTTCCAAAAAGCTGCAGAAAGG
  CAGAAAGTTAAAGAAGCTTCGCTTTCATCTCTTACTGCTCCCACTCAGGCTCCCATGAGCAATTCACCAT
  CCAAGCCCTCATTACCTTTTCAAACCAGTCAAAGTACAGGAACTGAGCCCTTCTTTAAGCAGAAAAGTCT
  GCTTCTAAAGCAGAAACAGCTTAATAATTCTTCAGTTTCTTCCCCCCAACAAAACCCATGGTCCAACTGT
  AAAGCATTACCAAACTCTTTACCAACAGAGTATCCAGGGTGTGTCCCTGTTTGTGAAGGGGTGTCGAAGC
  TAGAAGAATCCTCTAAAGCAACTCCTGCAGAGATGGATTTGGCCCACAACAGCCAAAGCATGCACGCCTC
  TTCAGCTTCCAAATCTGTGCTGGAGGTGACTCAGAAAGCAACCCCAAATCCAAGTCTTCTAGCTGCTGAG
  GACCAAGTGCCCTGTGAGAAGTGTGGCTCCCTGGTACCGGTATGGGATATGCCAGAACACATGGACTATC
  ATTTTGCATTGGAGTTGCAGAAATCCTTTTTGCAGCCCCACTCTTCAAACCCCCAGGTTGTTTCTGCCGT
  ATCTCATCAAGGCAAAAGAAATCCCAAGAGCCCTTTGGCCTGCACTAATAAACGCCCCAGGCCTGAGGGC
  ATGCAAACATTGGAATCATTTTTTAAGCCATTAACACATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006502
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006502.2, NP_006493.1
• RefSeq Size: 8412 bp
• RefSeq ORF: 2142 bp
• Locus ID: 5429
• Cytogenetics: 6p21.1
• Domains: IMS
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).