PLOD2 (NM_000935) Human Untagged Clone

CAT#: SC314581

PLOD2 (untagged)-Human procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLOD2
• Synonyms: BRKS2; LH2; TLH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC314581 sequence for NM_000935 edited (data generated by NextGen Sequencing)
  
  
  ATGGGGGGATGCACGGTGAAGCCTCAGCTGCTGCTCCTGGCGCTCGTCCTCCACCCCTGGAATCCCTGTC
  TGGGTGCGGACTCGGAGAAGCCCTCGAGCATCCCCACAGATAAATTATTAGTCATAACTGTAGCAACAAA
  AGAAAGTGATGGATTCCATCGATTTATGCAGTCAGCCAAATATTTCAATTATACTGTGAAGGTCCTTGGT
  CAAGGAGAAGAATGGAGAGGTGGTGATGGAATTAATAGTATTGGAGGGGGCCAGAAAGTGAGATTAATGA
  AAGAAGTCATGGAACACTATGCTGATCAAGATGATCTGGTTGTCATGTTTACTGAATGCTTTGATGTCAT
  ATTTGCTGGTGGTCCAGAAGAAGTTCTAAAAAAATTCCAAAAGGCAAACCACAAAGTGGTCTTTGCAGCA
  GATGGAATTTTGTGGCCAGATAAAAGACTAGCAGACAAGTATCCTGTTGTGCACATTGGGAAACGCTATC
  TGAATTCAGGAGGATTTATTGGCTATGCTCCATATGTCAACCGTATAGTTCAACAATGGAATCTCCAGGA
  TAATGATGATGATCAGCTCTTTTACACTAAAGTTTACATTGATCCACTGAAAAGGGAAGCTATTAACATC
  ACATTGGATCACAAATGCAAAATTTTCCAGACCTTAAATGGAGCTGTAGATGAAGTTGTTTTAAAATTTG
  AAAATGGCAAAGCCAGAGCTAAGAATACATTTTATGAAACATTACCAGTGGCAATTAATGGAAATGGACC
  CACCAAGATTCTCCTGAATTATTTTGGAAACTATGTACCCAATTCATGGACACAGGATAATGGCTGCACT
  CTTTGTGAATTCGATACAGTCGACTTGTCTGCAGTAGATGTCCATCCAAACGTATCAATAGGTGTTTTTA
  TTGAGCAACCAACCCCTTTTCTACCTCGGTTTCTGGACATATTGTTGACACTGGATTACCCAAAAGAAGC
  ACTTAAACTTTTTATTCATAACAAAGAAGTTTATCATGAAAAGGACATCAAGGTATTTTTTGATAAAGCT
  AAGCATGAAATCAAAACTATAAAAATAGTAGGACCAGAAGAAAATCTAAGTCAAGCGGAAGCCAGAAACA
  TGGGAATGGACTTTTGCCGTCAGGATGAAAAGTGTGATTATTACTTTAGTGTGGATGCAGATGTTGTTTT
  GACAAATCCAAGGACTTTAAAAATTTTGATTGAACAAAACAGAAAGATCATTGCTCCTCTTGTAACTCGT
  CATGGAAAGCTGTGGTCCAATTTCTGGGGAGCATTGAGTCCTGATGGATACTATGCACGATCTGAAGATT
  ATGTGGATATTGTTCAAGGGAATAGAGTAGGAGTATGGAATGTCCCATATATGGCTAATGTGTACTTAAT
  TAAAGGAAAGACACTCCGATCAGAGATGAATGAAAGGAACTATTTTGTTCGTGATAAACTGGATCCTGAT
  ATGGCTCTTTGCCGAAATGCTAGAGAAATGGGTGTATTTATGTACATTTCTAATAGACATGAATTTGGAA
  GGCTATTATCCACTGCTAATTACAATACTTCCCATTATAACAATGACCTCTGGCAGATTTTTGAAAATCC
  TGTGGACTGGAAGGAAAAGTATATAAACCGTGATTATTCAAAGATTTTCACTGAAAATATAGTTGAACAG
  CCCTGTCCAGATGTCTTTTGGTTCCCCATATTTTCTGAAAAAGCCTGTGATGAATTGGTAGAAGAAATGG
  AACATTACGGCAAATGGTCTGGGGGAAAACATCATGATAGCCGTATATCTGGTGGTTATGAAAATGTCCC
  AACTGATGATATCCACATGAAGCAAGTTGATCTGGAGAATGTATGGCTTCATTTTATCCGGGAGTTCATT
  GCACCAGTTACACTGAAGGTCTTTGCAGGCTATTATACGAAGGGATTTGCACTACTGAATTTTGTAGTAA
  AATACTCCCCTGAACGACAGCGTTCTCTTCGTCCTCATCATGATGCTTCTACATTTACCATAAACATTGC
  ACTTAATAACGTGGGAGAAGACTTTCAGGGAGGTGGTTGCAAATTTCTAAGGTACAATTGCTCTATTGAG
  TCACCACGAAAAGGCTGGAGCTTCATGCATCCTGGGAGACTCACACATTTGCATGAAGGACTTCCTGTTA
  AAAATGGAACAAGATACATTGCAGTGTCATTTATAGATCCCTAA
  
  Clone variation with respect to NM_000935.2
  
• Restriction Sites: Please inquire
• ACCN: NM_000935
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000935.2, NP_000926.2
• RefSeq Size: 4009 bp
• RefSeq ORF: 2214 bp
• Locus ID: 5352
• Cytogenetics: 3q24
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Pathways: Lysine degradation
• Gene Summary: 'The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an internal in-frame coding exon compared to transcript variant 1, resulting in a shorter isoform (2, also known as LH2a) missing a 21 aa protein segment compared to isoform 1.