HIF1 beta (ARNT) (NM_178427) Human Untagged Clone

CAT#: SC314664

ARNT (untagged)-Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARNT
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC314664 sequence for NM_178427 edited (data generated by NextGen Sequencing)
  
  
  ATGGCGGCGACTACTGCCAACCCCGAAATGACATCAGATGTACCATCACTGGGTCCAGCCATTGCCTCTG
  GAAACTCTGGACCTGGAATTCAAGGTGGAGGAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCT
  GGATTTTGATGATGATGGAGAAGGGAACAGTAAATTTTTGAGGTGTGATGATGATCAGATGTCTAACGAT
  AAGGAGCGGTTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGGCGACGGAACAAGATGACAGCCTACA
  TCACAGAACTGTCAGATATGGTACCCACCTGTAGTGCCCTGGCTCGAAAACCAGACAAGCTAACCATCTT
  ACGCATGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAACACATCCACTGATGGCTCCTATAAG
  CCGTCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTG
  TCTCATGTGAGACAGGCAGGGTGGTGTATGTGTCTGACTCCGTGACTCCTGTTTTGAACCAGCCACAGTC
  TGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGATGATGTGGATAAACTTCGTGAGCAGCTT
  TCCACTTCAGAAAATGCCCTGACAGGGCGTATCCTGGATCTAAAGACTGGAACAGTGAAAAAGGAAGGTC
  AGCAGTCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCGTTTATTTGCCGAATGAGGTGTGGCAGTAG
  CTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCTTTGTGAGGAACAGATGCAGGAATGGACTTGGCTCT
  GTAAAGGATGGGGAACCTCACTTCGTGGTGGTCCACTGCACAGGCTACATCAAGGCCTGGCCCCCAGCAG
  GTGTTTCCCTCCCAGATGATGACCCAGAGGCTGGCCAGGGAAGCAAGTTTTGCCTAGTGGCCATTGGCAG
  ATTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAATGTTTGTCAACCAACAGAGTTCATCTCC
  CGACACAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCTACTGTTGGCTACCAGCCAC
  AGGAACTCTTAGGAAAGAATATTGTAGAATTCTGTCATCCTGAAGACCAGCAGCTTCTAAGAGACAGCTT
  CCAACAGGTAGTGAAATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACCAAGAA
  TGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTACTCAGATGAAATTGAGTACATCATCT
  GTACCAACACCAATGTGAAGAACTCTAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAGGCC
  ACAACTAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGACAGCTGGCACCCAGGCAGCAGCAA
  CAGCAAACAGAATTGGACATGGTACCAGGAAGAGATGGACTGGCCAGCTACAATCATTCCCAGGTGGTTC
  AGCCTGTGACAACCACAGGACCAGAACACAGCAAGCCCCTTGAGAAGTCAGATGGTTTATTTGCCCAGGA
  TAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCGGATCAGAGTAAAGGCATCTCCTCCAGC
  ACTGTCCCTGCCACCCAACAGCTATTCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAGAGA
  ATTTCAGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTCCAGCCATCAGCTTCTGCAGGACAGATGTT
  GGCCCAGATTTCCCGCCACTCCAACCCCACCCAAGGAGCAACCCCAACTTGGACCCCTACTACCCGCTCA
  GGCTTTTCTGCCCAGCAGGTGGCTACCCAGGCTACTGCTAAGACTCGTACTTCCCAGTTTGGTGTGGGCA
  GCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTCCCTGGTGCCCCAACTGCATCGCCTGGTGCTGC
  TGCCTACCCTAGTCTCACCAATCGTGGATCTAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAATTC
  CAGACACGGACAGCAGAGGGTGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGCCTCATCATCGTTCAA
  GTTCTAGTGAGCAACATGTTCAACAACCGCCAGCACAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGAT
  GCTGTCCATGCTGGGAGATCAGAGCAACAGCTACAACAATGAAGAATTCCCTGATCTAACTATGTTTCCC
  CCCTTTTCAGAATAG
  
  Clone variation with respect to NM_178427.2
  
• Restriction Sites: Please inquire
• ACCN: NM_178427
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178427.1, NP_848514.1
• RefSeq Size: 4858 bp
• RefSeq ORF: 2325 bp
• Locus ID: 405
• Cytogenetics: 1q21.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.