SEL1L (NM_005065) Human Untagged Clone

CAT#: SC314718

SEL1L (untagged)-Human sel-1 suppressor of lin-12-like (C. elegans) (SEL1L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEL1L
• Synonyms: Hrd3; PRO1063; SEL1-LIKE; SEL1L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005065 edited
  ATGCGGGTCCGGATAGGGCTGACGCTGCTGCTGTGTGCGGTGCTGCTGAGCTTGGCCTCG
  GCGTCCTCGGATGAAGAAGGCAGCCAGGATGAATCCTTAGATTCCAAGACTACTTTGACA
  TCAGATGAGTCAGTAAAGGACCATACTACTGCAGGCAGAGTAGTTGCTGGTCAAATATTT
  CTTGATTCAGAAGAATCTGAATTAGAATCCTCTATTCAAGAAGAGGAAGACAGCCTCAAG
  AGCCAAGAGGGGGAAAGTGTCACAGAAGATATCAGCTTTCTAGAGTCTCCAAATCCAGAA
  AACAAGGACTATGAAGAGCCAAAGAAAGTACGGAAACCAGCTTTGACCGCCATTGAAGGC
  ACAGCACATGGGGAGCCCTGCCACTTCCCTTTTCTTTTCCTAGATAAGGAGTATGATGAA
  TGTACATCAGATGGGAGGGAAGATGGCAGACTGTGGTGTGCTACAACCTATGACTACAAA
  GCAGATGAAAAGTGGGGCTTTTGTGAAACTGAAGAAGAGGCTGCTAAGAGACGGCAGATG
  CAGGAAGCAGAAATGATGTATCAAACTGGAATGAAAATCCTTAATGGAAGCAATAAGAAA
  AGCCAAAAAAGAGAAGCATATCGGTATCTCCAAAAGGCAGCAAGCATGAACCATACCAAA
  GCCCTGGAGAGAGTGTCATATGCTCTTTTATTTGGTGATTACTTGCCACAGAATATCCAG
  GCAGCGAGAGAGATGTTTGAGAAGCTGACTGAGGAAGGCTCTCCCAAGGGACAGACTGCT
  CTTGGCTTTCTGTATGCCTCTGGACTTGGTGTTAATTCAAGTCAGGCAAAGGCTCTTGTA
  TATTATACATTTGGAGCTCTTGGGGGCAATCTAATAGCCCACATGGTTTTGGGTTACAGA
  TACTGGGCTGGCATCGGCGTCCTCCAGAGTTGTGAATCTGCCCTGACTCACTATCGTCTT
  GTTGCCAATCATGTTGCTAGTGATATCTCGCTAACAGGAGGCTCAGTAGTACAGAGAATA
  CGGCTGCCTGATGAAGTGGAAAATCCAGGAATGAACAGTGGAATGCTAGAAGAAGATTTG
  ATTCAATATTACCAGTTCCTAGCTGAAAAAGGTGATGTACAAGCACAGGTTGGTCTTGGA
  CAACTGCACCTGCACGGAGGGCGTGGAGTAGAACAGAATCATCAGAGAGCATTTGACTAC
  TTCAATTTAGCAGCAAATGCTGGCAATTCACATGCCATGGCCTTTTTGGGAAAGATGTAT
  TCAGAAGGAAGTGACATTGTACCTCAGAGTAATGAGACAGCTCTCCACTACTTTAAGAAA
  GCTGCTGACATGGGCAACCCAGTTGGACAGAGTGGGCTTGGAATGGCCTACCTCTATGGG
  AGAGGAGTTCAAGTTAATTATGATCTAGCCCTTAAGTATTTCCAGAAAGCTGCTGAACAA
  GGCTGGGTGGATGGGCAGCTACAGCTTGGTTCCATGTACTATAATGGCATTGGAGTCAAG
  AGAGATTATAAACAGGCCTTGAAGTATTTTAATTTAGCTTCTCAGGGAGGCCATATCTTG
  GCTTTCTATAACCTAGCTCAGATGCATGCCAGTGGCACCGGCGTGATGCGATCATGTCAC
  ACTGCAGTGGAGTTGTTTAAGAATGTATGTGAACGAGGCCGTTGGTCTGAAAGGCTTATG
  ACTGCCTATAACAGCTATAAAGATGGCGATTACAATGCTGCAGTGATCCAGTACCTCCTC
  CTGGCTGAACAGGGCTATGAAGTGGCACAAAGCAATGCAGCCTTTATTCTTGATCAGAGA
  GAAGCAAGCATTGTAGGTGAGAATGAAACTTATCCCAGAGCTTTGCTACATTGGAACAGG
  GCCGCCTCTCAAGGCTATACTGTGGCTAGAATTAAGCTCGGAGACTACCATTTCTATGGG
  TTTGGCACCGATGTAGATTATGAAACTGCATTTATTCATTACCGTCTGGCTTCTGAGCAG
  CAACACAGTGCACAAGCTATGTTTAATCTGGGATATATGCATGAGAAAGGACTGGGCATT
  AAACAGGATATTCACCTTGCGAAACGTTTTTATGACATGGCAGCTGAAGCCAGCCCAGAT
  GCACAAGTTCCAGTCTTCCTAGCCCTCTGCAAATTGGGCGTCGTCTATTTCTTGCAGTAC
  ATACGGGAAACAAACATTCGAGATATGTTCACCCAACTTGATATGGACCAGCTTTTGGGA
  CCTGAGTGGGACCTTTACCTCATGACCATCATTGCGCTGCTGTTGGGAACAGTCATAGCT
  TACAGGCAAAGGCAGCACCAAGACATGCCTGCACCCAGGCCTCCAGGGCCACGGCCAGCT
  CCACCCCAGCAGGAGGGGCCACCAGAGCAGCAGCCACCACAGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_005065
• Insert Size: 6600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005065.3, NP_005056.3
• RefSeq Size: 7885 bp
• RefSeq ORF: 2385 bp
• Locus ID: 6400
• Cytogenetics: 14q31.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).