Villin (VIL1) (NM_007127) Human Untagged Clone

CAT#: SC314785

VIL1 (untagged)-Human villin 1 (VIL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VIL1
• Synonyms: D2S1471; VIL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007127, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCAAGCTGAGCGCCCAAGTCAAAGGCTCTCTCAACATCACCACCCCGGGGCTGCAGATATGGAGGA
  TCGAGGCCATGCAGATGGTGCCTGTTCCTTCCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTACAT
  CATCCTGGCTATCCACAAGACAGCCAGCAGCCTGTCCTATGACATCCACTACTGGATTGGCCAGGACTCA
  TCCCTGGATGAGCAGGGGGCAGCTGCCATCTACACCACACAGATGGATGACTTCCTGAAGGGCCGGGCTG
  TGCAGCACCGCGAGGTCCAGGGCAACGAGAGCGAGGCCTTCCGAGGCTACTTCAAGCAAGGCCTTGTGAT
  CCGGAAAGGGGGCGTGGCTTCTGGCATGAAGCACGTGGAGACCAACTCCTATGACGTCCAGAGGCTGCTG
  CATGTCAAGGGCAAGAGGAACGTGGTAGCTGGAGAGGTAGAGATGTCCTGGAAGAGTTTCAACCGAGGGG
  ATGTTTTCCTCCTGGACCTTGGGAAGCTTATCATCCAGTGGAATGGACCGGAAAGCACCCGTATGGAGAG
  ACTCAGGGGCATGACTCTGGCCAAGGAGATCCGAGACCAGGAGCGGGGAGGGCGCACCTATGTAGGCGTG
  GTGGACGGAGAGAATGAATTGGCATCCCCGAAGCTGATGGAGGTGATGAACCACGTGCTGGGCAAGCGCA
  GGGAGCTGAAGGCGGCCGTGCCCGACACGGTGGTGGAGCCGGCACTCAAGGCTGCACTCAAACTGTACCA
  TGTGTCTGACTCCGAGGGGAATCTGGTGGTGAGGGAAGTCGCCACACGGCCACTGACACAGGACCTGCTC
  AGTCACGAGGACTGTTACATCCTGGACCAGGGGGGCCTGAAGATCTACGTGTGGAAAGGGAAGAAAGCCA
  ATGAGCAGGAGAAGAAGGGAGCCATGAGCCATGCGCTGAACTTCATCAAAGCCAAGCAGTACCCACCAAG
  CACACAGGTGGAGGTGCAGAATGATGGGGCTGAGTCGGCCGTCTTTCAGCAGCTCTTCCAGAAGTGGACA
  GCGTCCAACCGGACCTCAGGCCTAGGCAAAACCCACACTGTGGGCTCCGTGGCCAAAGTGGAACAGGTGA
  AGTTCGATGCCACATCCATGCATGTCAAGCCTCAGGTGGCTGCCCAGCAGAAGATGGTAGATGATGGGAG
  TGGGGAAGTGCAGGTGTGGCGCATTGAGAACCTAGAGCTGGTACCTGTGGATTCCAAGTGGCTAGGCCAC
  TTCTATGGGGGCGACTGCTACCTGCTGCTCTACACCTACCTCATCGGCGAGAAGCAGCATTACCTGCTCT
  ACGTTTGGCAGGGCAGCCAGGCCAGCCAAGATGAAATTACAGCATCAGCTTATCAAGCCGTCATCCTGGA
  CCAGAAGTACAATGGTGAACCAGTCCAGATCCGGGTCCCAATGGGCAAGGAGCCACCTCATCTTATGTCC
  ATCTTCAAGGGACGCATGGTGGTCTACCAGGGAGGCACCTCCCGAACTAACAACTTGGAGACCGGGCCCT
  CCACACGGCTGTTCCAGGTCCAGGGAACTGGCGCCAACAACACCAAGGCCTTTGAGGTCCCAGCGCGGGC
  CAATTTCCTCAATTCCAATGATGTCTTTGTCCTCAAGACCCAGTCTTGCTGCTATCTATGGTGTGGGAAG
  GGTTGTAGCGGGGACGAGCGGGAGATGGCCAAGATGGTTGCTGACACCATCTCCCGGACGGAGAAGCAAG
  TGGTGGTGGAAGGGCAGGAGCCAGCCAACTTCTGGATGGCCCTGGGTGGGAAGGCCCCCTATGCCAACAC
  CAAGAGACTACAGGAAGAAAACCTGGTCATCACCCCCCGGCTCTTTGAGTGTTCCAACAAGACTGGGCGC
  TTCCTGGCCACAGAGATCCCTGACTTCAATCAGGATGACTTGGAAGAGGATGATGTGTTCCTACTAGATG
  TCTGGGACCAGGTCTTCTTCTGGATTGGGAAACATGCCAACGAGGAGGAGAAGAAGGCCGCAGCAACCAC
  TGCACAGGAATACCTCAAGACCCATCCCAGCGGGCGTGACCCTGAGACCCCCATCATTGTGGTGAAGCAG
  GGACACGAGCCCCCCACCTTCACAGGCTGGTTCCTGGCTTGGGATCCCTTCAAGTGGAGTAACACCAAAT
  CCTATGAGGACCTGAAGGCGGAGCTTGGCAACTCTAGGGACTGGAGCCAGATCACTGCTGAGGTCACAAG
  CCCCAAAGTGGACGTGTTCAATGCTAACAGCAACCTCAGTTCTGGGCCTCTGCCCATCTTCCCCCTGGAG
  CAGCTAGTGAACAAGCCTGTAGAGGAGCTCCCCGAGGGTGTGGACCCCAGCAGGAAGGAGGAACACCTGT
  CCATTGAAGATTTCACTCAGGCCTTTGGGATGACTCCAGCTGCCTTCTCTGCTCTGCCTCGATGGAAGCA
  ACAAAACCTCAAGAAAGAAAAAGGACTATTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_007127
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007127.2, NP_009058.2
• RefSeq Size: 2759 bp
• RefSeq ORF: 2484 bp
• Locus ID: 7429
• Cytogenetics: 2q35
• Gene Summary: 'This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]'