MSH5 (NM_025259) Human Untagged Clone

CAT#: SC314816

MSH5 (untagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH5
• Synonyms: G7; MUTSH5; NG23; POF13
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_025259, the custom clone sequence may differ by one or more nucleotides
  ATGGCCTCCTTAGGAGCGAACCCAAGGAGGACACCGCAGGGACCGAGACCTGGGGCGGCC
  TCCTCCGGCTTCCCCAGCCCGGCCCCAGTGCCGGGCCCCAGGGAGGCCGAGGAGGAGGAA
  GTCGAGGAGGAGGAGGAGCTGGCCGAGATCCATCTGTGTGTGCTGTGGAATTCAGGATAC
  TTGGGCATTGCCTACTATGATACTAGTGACTCCACTATCCACTTCATGCCAGATGCCCCA
  GACCACGAGAGCCTCAAGCTTCTCCAGAGAGTTCTGGATGAGATCAATCCCCAGTCTGTT
  GTTACGAGTGCCAAACAGGATGAGAATATGACTCGATTTCTGGGAAAGCTTGCCTCCCAG
  GAGCACAGAGAGCCTAAAAGACCTGAAATCATATTTTTGCCAAGTGTGGATTTTGGTCTG
  GAGATAAGCAAACAACGCCTCCTTTCTGGAAACTACTCCTTCATCCCAGACGCCATGACT
  GCCACTGAGAAAATCCTCTTCCTCTCTTCCATTATTCCCTTTGACTGCCTCCTCACACCC
  CCAGGAGATTTAAGATTTACCCCGATTCCACTGCTGATCCCCTCCCAGGTTCGAGCACTT
  GGAGGGCTGCTGAAGTTCCTGGGTCGAAGAAGAATCGGGGTTGAACTGGAAGACTATAAT
  GTCAGCGTCCCCATCCTGGGCTTTAAGAAATTTATGTTGACTCATCTGGTGAACATAGAT
  CAAGACACTTACAGTGTTCTACAGATTTTTAAGAGTGAGTCTCACCCCTCAGTGTACAAA
  GTGGCCAGTGGACTGAAGGAGGGGCTCAGCCTCTTTGGAATCCTCAACAGATGCCACTGT
  AAGTGGGGAGAGAAGCTGCTCAGGCTATGGTTCACACGTCCGACTCATGACCTGGGGGAG
  CTCAGTTCTCGTCTGGACGTCATTCAGTTTTTTCTGCTGCCCCAGAATCTGGACATGGCT
  CAGATGCTGCATCGGCTCCTGGGTCACATCAAGAACGTGCCTCTGATTCTGAAACGCATG
  AAGTTGTCCCACACCAAGGTCAGCGACTGGCAGGTTCTCTACAAGACTGTGTACAGTGCC
  CTGGGCCTGAGGGATGCCTGCCGCTCCCTGCCGCAGTCCATCCAGCTCTTTCGGGACATT
  GCCCAAGAGTTCTCTGATGACCTGCACCATATCGCCAGCCTCATTGGGAAAGTAGTGGAC
  TTTGAGGGCAGCCTTGCTGAAAATCGCTTCACAGTCCTCCCCAACATAGATCCTGAAATT
  GATGAGAAAAAGCGAAGACTGATGGGACTTCCCAGTTTCCTTACTGAGGTTGCCCGCAAG
  GAGCTGGAGAATCTGGACTCCCGTATTCCTTCATGCAGTGTCATCTACATCCCTCTGATT
  GGCTTCCTTCTTTCTATTCCCCGCCTGCCTTCCATGGTAGAGGCCAGTGACTTTGAGATT
  AATGGACTGGACTTCATGTTTCTCTCAGAGGAGAAGCTGCACTATCGTAGTGCCCGAACC
  AAGGAGCTGGATGCATTGCTGGGGGACCTGCACTGCGAGATCCGGGACCAGGAGACGCTG
  CTGATGTACCAGCTACAGTGCCAGGTGCTGGCACGAGCAGCTGTCTTAACCCGAGTATTG
  GACCTTGCCTCCCGCCTGGACGTCCTGCTGGCTCTTGCCAGTGCTGCCCGGGACTATGGC
  TACTCAAGGCCGCGTTACTCCCCACAAGTCCTTGGGGTACGAATCCAGAATGGCAGACAT
  CCTCTGATGGAACTCTGTGCCCGAACCTTTGTGCCCAACTCCACAGAATGTGGTGGGGAC
  AAAGGGAGGGTCAAAGTCATCACTGGACCCAACTCATCAGGGAAGAGCATATACCTCAAA
  CAGGTAGGCTTGATCACATTCATGGCCCTGGTAGGCAGCTTTGTGCCAGCAGAGGAGGCC
  GAAATTGGGGCAGTAGACGCCATCTTCACACGAATTCATAGCTGCGAATCCATCTCCCTT
  GGCCTCTCCACCTTCATGATCGACCTCAACCAGGTGGCGAAAGCAGTGAACAATGCCACT
  GCACAGTCGCTGGTCCTTATTGATGAATTTGGAAAGGGAACCAACACGGTGGATGGGCTC
  GCGCTTCTGGCCGCTGTGCTCCGACACTGGCTGGCACGTGGACCCACATGCCCCCACATC
  TTTGTGGCCACCAACTTTCTGAGCCTTGTTCAGCTACAACTGCTGCCACAAGGGCCCCTG
  GTGCAGTATTTGACCATGGAGACCTGTGAGGATGGCAACGATCTTGTCTTCTTCTATCAG
  GTTTGCGAAGGTGTTGCGAAGGCCAGCCATGCCTCCCACACAGCTGCCCAGGCTGGGCTT
  CCTGACAAGCTTGTGGCTCGTGGCAAGGAGGTCTCAGACTTGATCCGCAGTGGAAAACCC
  ATCAAGCCTGTCAAGGATTTGCTAAAGAAGAACCAAATGGAAAATTGCCAGACATTAGTG
  GATAAGTTTATGAAACTGGATTTGGAAGATCCTAACCTGGACTTGAACGTTTTCATGAGC
  CAGGAAGTGCTGCCTGCTGCCACCAGCATCCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_025259
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025259.3, NP_079535.3
• RefSeq Size: 4019 bp
• RefSeq ORF: 2751 bp
• Locus ID: 4439
• Cytogenetics: 6p21.33
• Domains: MutS_V, MutS_III, MutS_IV
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (a).