alpha Adaptin (AP2A2) (BC006155) Human Untagged Clone

CAT#: SC314956

AP2A2 (untagged)-Homo sapiens, clone MGC:13188 IMAGE:3627239, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2A2
• Synonyms: adaptin, alpha B; adaptor-related protein complex 2, alpha 2 subunit; ADTAB; alpha-adaptin C; CLAPA2; clathrin-associated/assembly/adaptor protein, large, alpha 2; HIP9; Huntingtin interacting protein J; HYPJ
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC006155, the custom clone sequence may differ by one or more nucleotides
  ATGCCGGCCGTGTCCAAGGGGGACGGGATGCGGGGCCTGGCGGTCTTCATCTCGGATATC
  CGCAACTGTAAAAGTAAAGAAGCAGAAATAAAAAGGATAAACAAGGAACTGGCAAATATC
  AGATCAAAATTTAAAGGTGACAAGGCTCTTGATGGCTATAGTAAAAAAAAGTACGTCTGC
  AAGTTGCTCTTCATCTTTCTCCTTGGTCATGACATTGACTTTGGACACATGGAGGCTGTG
  AACCTGCTGAGTTCAAACAGATACACGGAAAAGCAGATCGGCTACCTTTTCATCTCTGTG
  TTGGTGAACTCAAACAGTGAGCTGATCCGCCTGATCAACAACGCCATCAAGAATGACCTG
  GCCAGCCGCAACCCCACCTTCATGGGCCTGGCCCTGCACTGCATCGCCAGCGTGGGCAGC
  CGGGAGATGGCCGAGGCCTTCGCCGGGGAGATCCCTAAGGTCCTCGTAGCCGGAGACACT
  ATGGACAGCGTGAAGCAGAGCGCGGCCCTGTGCTTGCTGCGCCTGTACAGGACGTCCCCC
  GATCTTGTCCCCATGGGCGACTGGACATCCCGAGTGGTGCACCTGCTCAATGACCAGCAC
  TTGGGTGTGGTAACTGCAGCCACAAGTCTGATCACCACTTTAGCACAGAAGAACCCAGAA
  GAGTTTAAAACCTCCGTGTCTCTGGCTGTCTCTAGGCTAAGCAGAATCGTGACGTCTGCA
  TCCACAGATCTCCAGGATTACACTTACTATTTTGTCCCGGCTCCCTGGCTGTCTGTCAAA
  CTGCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCTGCAGTGCGAGGCCGCCTGACT
  GAGTGCCTGGAGACCATCCTGAACAAAGCCCAAGAACCGCCCAAGTCGAAGAAGGTCCAG
  CACTCCAACGCGAAGAATGCCGTGCTCTTCGAGGCCATCAGCTTAATCATTCACCATGAC
  AGTGAGCCGAACCTGCTCGTCCGTGCCTGCAACCAGTTGGGCCAGTTTCTGCAGCACCGC
  GAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCTGAGTTC
  TCCCATGAGGCTGTCAAGACGCACATCGAGACGGTCATCAACGCCCTGAAGACTGAGCGG
  GACGTGAGCGTGCGGCAGCGGGCCGTGGACCTCCTCTACGCCATGTGCGACCGCAGCAAC
  GCCCCACAGATCGTGGCCGAGATGCTGAGCTATCTGGAGACAGCTGACTACTCCATCCGA
  GAAGAGATTGTGCTGAAGGTCGCCATCCTGGCTGAGAAGTACGCGGTGGACTACACCTGG
  TATGTGGATACCATCTTGAACTTGATCCGAATTGCTGGTGATTACGTGAGTGAAGAGGTG
  TGGTACCGAGTCATTCAGATCGTCATCAACCGGGACGACGTGCAGGGCTACGCGGCCAAG
  ACTGTGTTCGAGGCTCTTCAGGCTCCCGCGTGCCACGAGAACCTGGTCAAAGTGGGCGGC
  TACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGAGACCCGAGATCCAGCCCGCTGATC
  CAGTTCCACCTGCTGCACTCCAAGTTCCACCTGTGCAGCGTCCCCACCCGCGCGCTGCTC
  CTGTCCACCTACATCAAGTTCGTGAACCTCTTCCCGGAGGTGAAGCCCACCATCCAGGAC
  GTGCTGCGCAGCGACAGCCAGCTCAGGAACGCAGACGTGGAGCTGCAGCAGCGTGCTGTG
  GAGTACCTGCGGCTCAGCACCGTGGCCAGCACCGACATTCTGGCGACCGTGCTGGAGGAG
  ATGCCCCCATTCCCGGAGCGGGAGTCCTCCATCTTGGCAAAGCTCAAGAAGAAGAAGGGC
  CCCAGCACGGTGACAGACCTGGAGGACACCAAGCGGGACAGGAGTGTGGACGTGAACGGG
  GGTCCTGAGCCTGCCCCAGCCAGTACCAGCGCCGTGTCTACGCCTTCTCCGTCGGCAGAC
  CTGCTGGGTCTCGGGGCTGCCCCCCCTGCCCCCGCGGGCCCCCCACCCTCCTCCGGCGGC
  AGCGGGCTGCTCGTGGACGTGTTCTCAGACTCGGCCTCTGTGGTCGCGCCTCTCGCTCCT
  GGCTCCGAAGACAACTTTGCCAGGTTTGTTTGTAAAAACAATGGTGTGTTGTTTGAAAAC
  CAGCTGCTTCAAATTGGACTTAAGTCTGAATTTCGGCAGAATTTAGGTCGGATGTTTATC
  TTTTATGGTAATAAGACCTCCACGCAGTTCCTAAACTTTACCCCAACACTAATCTGTTCA
  GACGACCTTCAGCCTAACCTGAACCTGCAGACCAAGCCCGTGGACCCGACCGTGGAGGGG
  GGCGCGCAGGTGCAGCAGGTGGTCAACATAGAGTGCGTGTCCGACTTCACGGAGGCGCCA
  GTCCTCAACATTCAGTTCAGGTATGGGGGCACCTTCCAGAACGTGTCTGTGCAGCTGCCC
  ATCACTCTCAACAAATTCTTCCAGCCGACAGAAATGGCTTCTCAGGATTTCTTTCAACGT
  TGGAAGCAGTTGAGCAATCCACAGCAGGAAGTGCAGAACATCTTCAAAGCAAAGCACCCA
  ATGGACACAGAAGTCACCAAAGCCAAGATCATTGGATTTGGTTCTGCACTTCTTGAAGAA
  GTTGATCCTAATCCTGCGAATTTCGTGGGAGCTGGAATCATCCACACGAAAACCACCCAG
  ATTGGATGCCTGCTGCGCTTGGAGCCGAACCTGCAAGCCCAGATGTACCGGCTCACGCTG
  CGCACAAGTAAGGAAGCCGTTTCTCAGAGATTATGTGAATTGCTCTCAGCGCAGTTT
  
• Restriction Sites: Please inquire
• ACCN: BC006155
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006155.1, AAH06155.1
• RefSeq Size: 3275 bp
• RefSeq ORF: 2820 bp
• Locus ID: 161
• Cytogenetics: 11p15.5
• Domains: Adaptin_N, Alpha_adaptin_C, Alpha_adaptinC2
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]'