ARHGAP4 (NM_001666) Human Untagged Clone

CAT#: SC314968

ARHGAP4 (untagged)-Human Rho GTPase activating protein 4 (ARHGAP4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP4
• Synonyms: C1; p115; RGC1; RhoGAP4; SrGAP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001666, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGCTCACGGGAAGCTGCGGCGGGAGCGGGGGCTGCAGGCTGAGTATGAGACGCAAGTCAAAGAGA
  TGCGCTGGCAGCTGAGCGAGCAGCTGCGCTGCCTGGAGCTGCAGGGCGAGCTGCGGCGGGAGTTGCTGCA
  GGAGCTGGCAGAGTTCATGCGGCGCCGCGCTGAGGTGGAGCTGGAATACTCCCGGGGCCTGGAAAAGCTG
  GCCGAGCGCTTCTCCAGCCGTGGAGGCCGCCTGGGGAGCAGCCGGGAGCACCAAAGCTTCCGGAAGGAGC
  CGTCCCTCCTGTCGCCCTTGCACTGCTGGGCGGTGCTGCTGCAGCACACGCGGCAGCAGAGCCGGGAGAG
  CGCGGCCCTGAGTGAGGTGCTGGCCGGGCCCCTGGCCCAGCGCCTGAGTCACATTGCAGAGGACGTGGGG
  CGCCTGGTCAAGAAGAGCAGGGATCTGGAGCAGCAGCTGCAGGATGAGCTCCTGGAGGTGGTCTCAGAGC
  TCCAGACGGCCAAGAAGACGTACCAGGCATATCACATGGAGAGCGTGAATGCCGAGGCCAAGCTCCGGGA
  GGCCGAGCGGCAGGAGGAGAAGCGGGCAGGCCGGAGTGTCCCCACCACCACCGCTGGTGCCACTGAGGCA
  GGGCCCCTCCGCAAGAGCTCCCTCAAGAAGGGAGGGAGGCTGGTGGAGAAGCGGCAGGCCAAGTTCATGG
  AGCACAAACTCAAGTGCACAAAGGCGCGCAACGAGTACCTGCTTAGCCTGGCTAGTGTCAACGCTGCTGT
  CAGTAACTACTACCTGCATGACGTCTTGGACCTCATGGACTGCTGTGACACAGGGTTCCACCTGGCCCTG
  GGGCAGGTGCTCCGGAGCTACACGGCCGCTGAGAGCCGCACCCAAGCCTCCCAAGTGCAGGGCCTGGGCA
  GCCTGGAAGAAGCTGTGGAGGCCCTGGATCCTCCAGGGGACAAAGCCAAGGTTCTCGAGGTGCATGCTAC
  CGTCTTCTGTCCCCCGCTGCGCTTTGACTACCACCCCCATGATGGGGATGAGGTGGCTGAGATCTGCGTT
  GAAATGGAGCTGCGGGACGAGATTCTGCCCAGAGCCCAGAACATCCAGAGCCGCCTGGACCGACAGACCA
  TTGAGACAGAGGAGGTGAACAAGACTCTGAAGGCGACACTGCAGGCCCTGCTGGAGGTGGTGGCCTCGGA
  TGACGGGGATGTGCTTGATTCCTTCCAGACCAGCCCCTCCACCGAGTCCCTCAAGTCCACCAGCTCAGAC
  CCAGGCAGCCGGCAGGCGGGCCGGAGGCGCGGCCAGCAGCAGGAGACCGAAACCTTCTACCTCACGAAGC
  TCCAGGAGTATCTGAGTGGACGGAGCATCCTCGCCAAGCTGCAGGCCAAGCACGAGAAGCTGCAGGAGGC
  CCTTCAGCGAGGTGACAAGGAGGAGCAGGAGGTGTCTTGGACCCAGTACACACAGAGAAAATTCCAGAAG
  AGCCGCCAGCCCCGCCCCAGCTCCCAGTATAACCAGAGACTCTTTGGGGGAGACATGGAGAAGTTTATCC
  AGAGCTCAGGCCAGCCTGTGCCCCTGGTGGTGGAGAGCTGCATTCGCTTCATCAACCTCAATGGCCTGCA
  GCATGAAGGCATCTTCCGGGTATCGGGTGCCCAGCTCCGGGTCTCAGAGATCCGTGATGCCTTCGAGAGA
  GGGGAGGACCCACTGGTGGAGGGCTGCACTGCCCATGACCTGGACTCGGTGGCCGGGGTGCTGAAGCTCT
  ACTTCCGGAGCCTGGAGCCCCCACTCTTCCCCCCAGACCTGTTCGGCGAGCTGCTGGCTTCTTCGGAGCT
  GGAGGCCACAGCGGAGAGGGTGGAGCACGTGAGCCGCCTGCTGTGGCGGCTGCCCGCGCCGGTGCTGGTG
  GTTCTGCGCTACCTCTTCACCTTCCTCAACCACCTGGCCCAGTACAGCGATGAGAACATGATGGACCCCT
  ACAACCTGGCCGTGTGCTTCGGGCCCACGCTGCTACCGGTGCCCGCTGGGCAGGACCCGGTGGCGCTGCA
  GGGCCGGGTGAACCAGCTGGTGCAGACGCTCATAGTGCAGCCCGATCGGGTCTTCCCGCCCCTGACCTCG
  CTGCCTGGCCCCGTCTACGAGAAGTGCATGGCACCGCCTTCCGCCAGCTGCCTGGGGGACGCCCAGCTGG
  AGAGCCTGGGGGCGGACAATGAGCCGGAGCTGGAAGCCGAGATGCCCGCACAGGAGGATGACCTGGAGGG
  GGTCGTGGAGGCTGTGGCCTGCTTTGCCTACACGGGCCGCACAGCCCAGGAGCTGAGCTTCCGGCGGGGG
  GACGTACTGCGGCTGCACGAGAGGGCCTCGAGCGACTGGTGGCGGGGGGAGCACAACGGCATGCGGGGCC
  TCATCCCCCACAAGTATATCACGCTGCCCGCCGGGACGGAGAAGCAGGTGGTGGGCGCAGGGCTGCAGAC
  TGCAGGGGAGTCTGGGAGCAGTCCCGAGGGCCTCCTGGCATCGGAGCTGGTCCACCGGCCAGAGCCATGC
  ACCTCACCTGAGGCCATGGGACCCTCTGGACACAGACGACGCTGCTTGGTCCCAGCCTCCCCAGAGCAAC
  ACGTGGAGGTGGATAAGGCTGTGGCACAGAACATGGACTCTGTGTTTAAGGAGCTCTTGGGAAAGACCTC
  TGTCCGCCAGGGCCTTGGGCCAGCATCTACCACCTCTCCCAGTCCTGGGCCCCGAAGCCCAAAGGCCCCG
  CCCAGCAGCCGCCTGGGCAGGAACAAAGGCTTCTCCCGGGGCCCTGGGGCCCCAGCCTCACCCTCAGCTT
  CCCACCCCCAGGGCCTAGACACGACCCCCAAGCCACACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001666
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001666.4, NP_001657.3
• RefSeq Size: 3285 bp
• RefSeq ORF: 2841 bp
• Locus ID: 393
• Cytogenetics: Xq28
• Domains: RhoGAP, FCH, SH3
• Gene Summary: 'This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an alternate segment, compared to isoform 1.