GRF2 (RAPGEF1) (NM_005312) Human Untagged Clone

CAT#: SC315151

RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAPGEF1
• Synonyms: C3G; GRF2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005312, the custom clone sequence may differ by one or more nucleotides
  ATGGACACAGACTCTCAGCGTTCTCATCTCTCTTCCTTCACCATGAAGCTGATGGACAAA
  TTCCACTCACCCAAAATCAAGAGAACGCCATCAAAGAAGGGAAAACCAGCTGAGGTGTCC
  GTAAAGATTCCAGAGAAGCCTGTGAACAAAGAGGCAACAGACAGATTTCTACCAGAGGGC
  TACCCTCTCCCCTTGGATCTGGAGCAGCAGGCAGTAGAATTTATGTCCACCAGTGCTGTG
  GCTTCCAGGTCTCAAAGGCAGAAGAACCTGAGCTGGCTGGAGGAGAAAGAGAAGGAAGTT
  GTCAGTGCCCTGCGCTACTTTAAGACCATTGTGGACAAAATGGCAATTGATAAGAAGGTA
  CTGGAGATGCTTCCAGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAG
  AACGATCCTCGAATTCAGCACAGCTCAGCCCTCTCTTCCTGCTATAGCCGAGTGTACCAA
  AGCCTCGCCAACCTCATTCGCTGGTCTGACCAAGTGATGCTGGAAGGCGTGAACTCAGAA
  GACAAGGAGATGGTGACGACTGTGAAGGGGGTCATCAAGGCTGTGCTGGATGGAGTGAAG
  GAGCTGGTCAGGCTCACCATCGAGAAGCAGGGACGTCCGTCTCCGACGAGCCCCGTGAAG
  CCCAGTTCCCCTGCCAGCAAGCCTGATGGCCCAGCAGAGCTCCCCCTGACAGACCGCGAG
  GTAGAGATCCTAAACAAGACGACTGGGATGTCACAGTCAACTGAGCTCCTCCCAGATGCC
  ACGGATGAAGAGGTCGCGCCCCCCAAGCCTCCTCTGCCTGGCATTCGGGTGGTTGATAAT
  AGTCCTCCACCAGCATTGCCACCCAAGAAAAGACAGTCGGCGCCGTCCCCTACCCGAGTG
  GCTGTGGTGGCCCCCATGAGCCGAGCCACCAGTGGCTCCAGTTTGCCTGTTGGAATCAAT
  AGGCAGGATTTTGATGTTGACTGTTACGCACAGAGGCGACTGTCAGGAGGCAGCCACTCA
  TATGGTGGAGAGTCGCCCCGCCTCTCCCCCTGCAGCAGCATAGGCAAGCTCAGCAAGTCA
  GACGAGCAGCTGTCCTCTCTGGACAGGGACAGTGGGCAGTGCTCCCGGAACACAAGCTGT
  GAAACACTAGACCACTATGATCCCGACTATGAATTCCTCCAGCAAGACCTCTCTAACGCA
  GACCAGATACCTCAGCAGACGGCCTGGAACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAG
  TCTGGGTCTCCATTTCTTGGCCCTCCTTTCCAGCTGCCTCTTGGCGGCCATCCCCAGCCA
  GACGGACCTCTGGCCCCAGGGCAGCAGACAGATACGCCACCTGCTCTCCCCGAGAAGAAG
  CGCAGGAGCGCAGCCTCCCAGACGGCGGACGGCTCTGGCTGCAGGGTGTCCTACGAGCGG
  CATCCCTCGCAGTATGACAACATCTCTGGGGAGGACCTGCAGAGCACAGCCCCGATCCCA
  TCCGTCCCCTACGCGCCCTTTGCTGCTATTCTGCCCTTTCAGCATGGAGGTTCCTCAGCC
  CCTGTCGAATTTGTGGGTGATTTTACTGCTCCTGAGTCAACCGGTGACCCAGAAAAACCA
  CCTCCTCTACCAGAGAAGAAAAACAAACACATGCTGGCCTACATGCAGTTGCTGGAGGAC
  TACTCGGAGCCGCAGCCCTCTATGTTCTACCAGACGCCACAGAACGAGCACATCTACCAG
  CAGAAGAACAAGCTCCTCATGGAGGTATACGGCTTCAGCGACTCCTTCAGTGGGGTGGAC
  TCCGTGCAGGAGCTGGCCCCGCCGCCCGCCCTACCCCCCAAGCAGCGGCAGCTGGAGCCA
  CCGGCTGGGAAAGACGGACATCCCAGAGATCCCTCAGCGGTCAGCGGCGTCCCTGGGAAG
  GACAGCAGAGACGGCAGTGAGAGGGCCCCAAAGTCACCAGATGCTCTGGAGTCGGCTCAG
  TCGGAGGAGGAAGTGGACGAGCTGTCCCTCATTGACCACAACGAAATTATGTCCAGGCTG
  ACGCTCAAGCAGGAGGGTGATGACGGGCCGGACGTCCGCGGAGGATCTGGGGACATCTTA
  CTGGTCCATGCTACTGAGACTGACAGGAAAGATTTGGTGTTGTACTGCGAGGCATTCCTG
  ACCACCTACAGGACCTTCATCTCCCCAGAGGAGCTCATCAAGAAGCTGCAGTACAGATAT
  GAGAAATTCTCTCCCTTTGCCGACACATTCAAGAAGCGCGTCAGCAAGAACACGTTCTTC
  GTGCTGGTACGGGTGGTGGATGAGCTCTGCCTGGTGGAGTTGACAGAAGAGATCCTGAAG
  CTGCTGATGGAACTGGTCTTCCGCCTGGTGTGCAATGGGGAGCTGAGCCTGGCCCGTGTG
  CTCCGGAAGAACATCCTGGACAAGGTGGACCAGAAGAAGCTACTCAGGTGTGCCACCTCC
  AGCCAGCCCCTGGCAGCCCGGGGGGTAGCAGCCAGGCCGGGGACCTTGCACGACTTTCAC
  AGCCATGAGATAGCGGAGCAGCTAACGCTGCTGGATGCTGAGCTCTTCTATAAAATAGAG
  ATTCCTGAGGTTTTGCTTTGGGCAAAAGAGCAGAATGAGGAGAAGAGCCCCAACTTGACC
  CAGTTCACGGAGCACTTCAACAACATGTCCTACTGGGTCCGGTCCATAATCATGTTACAG
  GAAAAGGCCCAGGACAGGGAACGGCTGCTCTTGAAGTTCATCAAGATCATGAAGCACTTG
  CGGAAGCTGAATAACTTCAACTCCTACTTGGCCATCCTCTCTGCCCTGGACTCGGCGCCC
  ATCCGCAGGCTGGAGTGGCAGAAGCAGACTTCAGAGGGCCTGGCCGAGTACTGCACACTG
  ATCGACAGCTCGTCCTCCTTCCGAGCCTACCGGGCCGCCCTCTCGGAGGTGGAACCGCCG
  TGCATCCCGTACCTGGGGCTGATCCTGCAGGACCTGACCTTCGTTCACCTGGGAAACCCA
  GACTACATCGACGGGAAAGTGAACTTCTCCAAGCGGTGGCAGCAGTTCAACATCCTCGAC
  AGCATGCGCTGCTTCCAGCAGGCGCACTATGACATGCGGAGGAACGACGACATTATAAAC
  TTCTTCAATGACTTCAGTGACCACCTGGCTGAGGAGGCCCTATGGGAACTGTCTCTGAAA
  ATTAAACCCAGGAACATAACAAGGAGAAAAACAGACCGGGAAGAGAAGACC
  
• Restriction Sites: Please inquire
• ACCN: NM_005312
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005312.2, NP_005303.2
• RefSeq Size: 6121 bp
• RefSeq ORF: 3234 bp
• Locus ID: 2889
• Cytogenetics: 9q34.13
• Domains: RasGEFN, RasGEF
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
• Gene Summary: 'This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) uses an alternate 5'-terminal exon which results in a distinct 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (a) has a shorter and distinct N-terminus, compared to isoform b.