Desmoglein 2 (DSG2) (NM_001943) Human Untagged Clone

CAT#: SC315197

DSG2 (untagged)-Human desmoglein 2 (DSG2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DSG2
• Synonyms: CDHF5; HDGC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001943 edited
  ATGGCGCGGAGCCCGGGACGCGCGTACGCCCTGCTGCTTCTCCTGATCTGCTTTAACGTT
  GGAAGTGGACTTCACTTACAGGTCTTAAGCACAAGAAATGAAAATAAGCTGCTTCCTAAA
  CATCCTCATTTAGTGCGGCAAAAGCGCGCCTGGATCACCGCCCCCGTGGCTCTTCGGGAG
  GGAGAGGATCTGTCCAAGAAGAATCCAATTGCCAAGATACATTCTGATCTTGCAGAAGAA
  AGAGGACTCAAAATTACTTACAAATACACTGGAAAAGGGATTACAGAGCCACCTTTTGGT
  ATATTTGTCTTTAACAAAGATACTGGAGAACTGAATGTTACCAGCATTCTTGATCGAGAA
  GAAACACCATTTTTTCTGCTAACAGGTTACGCTTTGGATGCAAGAGGAAACAATGTAGAG
  AAACCCTTAGAGCTACGCATTAAGGTTCTTGATATCAATGACAACGAACCAGTGTTCACA
  CAGGATGTCTTTGTTGGGTCTGTTGAAGAGTTGAGTGCAGCACATACTCTTGTGATGAAA
  ATCAATGCAACAGATGCAGATGAGCCCAATACCCTGAATTCGAAAATTTCCTATAGAATC
  GTATCTCTGGAGCCTGCTTATCCTCCAGTGTTCTACCTAAATAAAGATACAGGAGAGATT
  TATACAACCAGTGTTACCTTGGACAGAGAGGAACACAGCAGCTACACTTTGACAGTAGAA
  GCAAGAGATGGCAATGGAGAAGTTACAGACAAACCTGTAAAACAAGCTCAAGTTCAGATT
  CGTATTTTGGATGTCAATGACAATATACCTGTAGTAGAAAATAAAGTGCTTGAAGGGATG
  GTTGAAGAAAATCAAGTCAACGTAGAAGTTACGCGCATAAAAGTGTTCGATGCAGATGAA
  ATAGGTTCTGATAATTGGCTGGCAAATTTTACATTTGCATCAGGAAATGAAGGAGGTTAT
  TTCCACATAGAAACAGATGCTCAAACTAACGAAGGAATTGTGACCCTTATTAAGGAAGTA
  GATTATGAAGAAATGAAGAATCTTGACTTCAGTGTTATTGTCGCTAATAAAGCAGCTTTT
  CACAAGTCGATTAGGAGTAAATACAAGCCTACACCCATTCCCATCAAGGTCAAAGTGAAA
  AATGTGAAAGAAGGCATTCATTTTAAAAGCAGCGTCATCTCAATTTATGTTAGCGAGAGC
  ATGGATAGATCAAGCAAAGGCCAAATAATTGGAAATTTTCAAGCTTTTGATGAGGACACT
  GGACTACCAGCCCATGCAAGATATGTAAAATTAGAAGATAGAGATAATTGGATCTCTGTG
  GATTCTGTCACATCTGAAATTAAACTTGCAAAACTTCCTGATTTTGAATCTAGATATGTT
  CAAAATGGCACATACACTGTAAAGATTGTGGCCATATCAGAAGATTATCCTAGAAAAACC
  ATCACTGGCACAGTCCTTATCAATGTTGAAGACATCAACGACAACTGTCCCACACTGATA
  GAGCCTGTGCAGACAATCTGTCACGATGCAGAGTATGTGAATGTTACTGCAGAGGACCTG
  GATGGACACCCAAACAGTGGCCCTTTCAGTTTCTCCGTCATTGACAAACCACCTGGCATG
  GCAGAAAAATGGAAAATAGCACGCCAAGAAAGTACCAGTGTGCTGCTGCAACAAAGTGAG
  AAAAAGCTTGGGAGAAGTGAAATTCAGTTCCTGATTTCAGACAATCAGGGTTTTAGTTGT
  CCTGAAAAGCAGGTCCTTACACTCACAGTTTGTGAGTGTCTGCATGGCAGCGGCTGCAGG
  GAAGCACAGCATGACTCCTATGTGGGCCTGGGACCCGCAGCAATTGCGCTCATGATTTTG
  GCCTTTCTGCTCCTGCTATTGGTACCACTTTTACTGCTGATGTGCCATTGCGGAAAGGGC
  GCCAAAGGCTTTACCCCCATACCTGGCACCATAGAGATGCTGCATCCTTGGAATAATGAA
  GGAGCACCACCTGAAGACAAGGTGGTGCCATCATTTCTGCCAGTGGATCAAGGGGGCAGT
  CTAGTAGGAAGAAATGGAGTAGGAGGTATGGCCAAGGAAGCCACGATGAAAGGAAGTAGC
  TCTGCTTCCATTGTCAAAGGGCAACATGAGATGTCCGAGATGGATGGAAGGTGGGAAGAA
  CACAGAAGCCTGCTTTCTGGTAGAGCTACCCAGTTTACAGGGGCCACAGGCGCTATCATG
  ACCACTGAAACCACGAAGACCGCAAGGGCCACAGGGGCTTCCAGAGACATGGCCGGAGCT
  CAGGCAGCTGCTGTTGCACTGAACGAAGAATTCTTAAGAAATTATTTCACTGATAAAGCG
  GCCTCTTACACTGAGGAAGATGAAAATCACACAGCCAAAGATTGCCTTCTGGTTTATTCT
  CAGGAAGAAACTGAATCGCTGAATGCTTCTATTGGTTGTTGCAGTTTTATTGAAGGAGAG
  CTAGATGACCGCTTCTTAGATGATTTGGGACTTAAATTCAAGACACTAGCTGAAGTTTGC
  CTGGGTCAAAAAATAGATATAAATAAGGAAATTGAGCAGAGACAAAAACCTGCCACAGAA
  ACAAGTATGAACACAGCTTCACATTCACTCTGTGAGCAAACTATGGTTAATTCAGAGAAT
  ACCTACTCCTCTGGCAGTAGCTTCCCAGTTCCAAAATCTTTGCAAGAAGCCAATGCAGAG
  AAAGTAACTCAGGAAATAGTCACTGAAAGATCTGTGTCTTCTAGGCAGGCGCAAAAGGTA
  GCTACACCTCTTCCTGACCCAATGGCTTCTAGAAATGTGATAGCAACAGAAACTTCCTAT
  GTCACAGGGTCCACTATGCCACCAACCACTGTGATCCTGGGTCCTAGCCAGCCACAGAGC
  CTTATTGTGACAGAGAGGGTGTATGCTCCAGCTTCTACCTTGGTAGATCAGCCTTATGCT
  AATGAAGGTACAGTTGTGGTCACTGAAAGAGTAATACAGCCTCATGGGGGTGGATCGAAT
  CCTCTGGAAGGCACTCAGCATCTTCAAGATGTACCTTACGTCATGGTGAGGGAAAGAGAG
  AGCTTCCTTGCCCCCAGCTCAGGTGTGCAGCCTACTCTGGCCATGCCTAATATAGCAGTA
  GGACAGAATGTGACAGTGACAGAAAGAGTTCTAGCACCTGCTTCCACTCTGCAATCCAGT
  TACCAGATTCCCACTGAAAATTCTATGACGGCTAGGAACACCACGGTGTCTGGAGCTGGA
  GTCCCTGGCCCTCTGCCAGATTTTGGTTTAGAGGAATCTGGTCATTCTAATTCTACCATA
  ACCACATCTTCCACCAGAGTTACCAAGCATAGCACTGTACAGCATTCTTACTCCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001943 unedited
  GAGCACTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGAGCGGTGCGG
  CGGCGGGAGGCGGAGGCGAGGGTGCGATGGCGCGGAGCCCGGGACGCGCGTACGCCCTGC
  TGCTTCTCCTGATCTGCTTTAACGTTGGAAGTGGACTTCACTTACAGGTCTTAAGCACAA
  GAAATGAAAATAAGCTGCTTCCTAAACATCCTCATTTAGTGCGGCAAAAGCGCGCCTGGA
  TCACCGCCCCCGTGGCTCTTCGGGAGGGAGAGGATCTGTCCAAGAAGAATCCAATTGCCA
  AGATACATTCTGATCTTGCAGAAGAAAGAGGACTCAAAATTACTTACAAATACACTGGAA
  AAGGGATTACAGAGCCACCTTTTGGTATATTTGTCTTTAACAAAGATACTGGAGAACTGA
  ATGTTACCAGCATTCTTGATCGAGAAGAAACACCATTTTTTCTGCTAACAGGTTACGCTT
  TGGATGCAAGAGGAAACAATGTAGAGAAACCCTTAGAGCTACGCATTAAGGTTCTTGATA
  TCAATGACAACGAACCAGTGTTCACACAGGATGTCTTTGTTGGGTCTGTTGAAGAGTTGA
  GTGCAGCACATACTCTTGTGATGAAAATCAATGCAACAGATGCAGATGAGCCCAATACCC
  TGAATTCGAAAATTTCCTATAGAATCGTATCTCTGGAGCCTGCTTATCCTCCAGTGTTCT
  ACCTAAATAAAGATACAGGAGAGATTTATACAACCAGTGTTACCTTGGACAGAGAGGAAC
  ACAGCAGCTACACTTTGACAGTAGAGCAGAGATGGCATGGAGAAGTTACAGACAAACCTG
  TAAACAGCTCAGTTCAGATTCGTATTTTGGATGTCAATGACATATACCTTGTAGTAGAAA
  ATAAAGTGC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001943 unedited
  AATGGATTAATAAGGAAATCTGAGCAGAGACAAAAACTCCTGCCACAGAAACAAGCTATG
  AACACAGCTTCACATTCACTCTGTGAGCAAACTATGGTTAATTCAGAGAATACCTACTCC
  TCTGGCAGTAGCTTCCCAGTTCCAAAATCTTTGCAAGAAGCCAATGCAGAGAAAGTAACT
  CAGGAAATAGTCACTGAAAGAACTGTGTCTTCTAGGCAGGCGCAAAAGGTAGCTACACCT
  CTTCCTGACCCAATGGCTTCTAGAAATGTGATAGCAACAGAAACTTCCTATGTCACAGGG
  TCCACTATGCCACCAACCACTGTGACCCTGGGTCCTAGCCAGCCACAGAGCCTTATTGTG
  ACAGAGAGGGTGTATGCTCCAGCTTCTACCTTGGTAGATCAGCCTTATGCTAATGAAGGT
  ACAGTTGTGGTCACTGAAAGAGTAATACAGCCTCATGGGGGTGGATCGAATCCTCTGGAA
  GGCACTCAGCATCTTCAAGATGTACCTTACGTCATGGTGAGGGAAAGAGAGAGCTTCCTT
  GCCCCCAGCTCAGGTGTGCAGCCTACTCTGGCCATGCCTAATATAGCAGTAGGACAGAAT
  GTGACAGTGACAGAAAGAGTTCTAGCACCTGCTTCCACTCTGCAATCCAGTTACCAGATT
  CCCACTGAAAATTCTATGACGGCTAGGAACACCACGGTGTCTGGAGCTGGAGTCCCTGGC
  CCTCTGCCAGATTTTGGTTTAGAGGAATCTGGTCATTCTAATTCTACCATAACCACATCT
  TCCACCAGAGTCACCAAGCATAGCACTGTACAGCATTCTTACTCCTAAACAGCAGTCAGC
  CACAAACTGACCCAGAGTTTATTAGCAGTGACTAATTTCATGTTTCCATGTACCTGATTT
  TTCATGAGCCTTACAGACCCACAGAGAACCCATACACATTGATTCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_001943
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001943.1, NP_001934.1
• RefSeq Size: 3516 bp
• RefSeq ORF: 3354 bp
• Locus ID: 1829
• Cytogenetics: 18q12.1
• Domains: CA
• Protein Families: Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Gene Summary: 'This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jan 2016]'