RASGRF2 (NM_006909) Human Untagged Clone

CAT#: SC315292

RASGRF2 (untagged)-Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRF2
• Synonyms: GRF2; RAS-GRF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006909 edited
  GAGGCAGGGGTGAGACCGGCGGCCACCCGTGAGCCCTCCGCACCCGCACCATGCAGAAGA
  GCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCTTTCTGGCGCGCAAGGAGGGCA
  CCAAGCGCGGCTTCCTGAGTAAGAAGACGGCCGAGGCGAGCCGCTGGCACGAGAAGTGGT
  TCGCCCTCTACCAGAATGTGCTCTTCTACTTCGAGGGCGAGCAGAGCTGCCGCCCGGCGG
  GCATGTACCTCCTGGAGGGCTGCAGCTGCGAACGAACGCCCGCGCCACCCAGGGCCGGCG
  CCGGGCAGGGAGGCGTCCGAGACGCGCTGGACAAGCAGTATTACTTTACTGTTCTTTTTG
  GCCATGAAGGTCAGAAGCCACTGGAGCTGCGCTGTGAGGAGGAGCAGGATGGTAAAGAGT
  GGATGGAGGCCATTCACCAAGCCAGTTATGCAGACATTTTGATTGAGAGGGAAGTATTAA
  TGCAGAAGTACATTCATCTAGTTCAGATCGTAGAGACAGAAAAAATTGCAGCTAACCAAC
  TCCGACATCAACTTGAAGATCAAGACACAGAAATCGAAAGGCTTAAATCAGAGATTATTG
  CTCTTAATAAAACCAAAGAACGAATGCGACCTTACCAAAGCAACCAAGAAGACGAAGATC
  CAGACATCAAGAAGATTAAAAAGGTTCAGAGCTTCATGCGAGGATGGTTGTGCAGAAGGA
  AATGGAAGACCATCGTGCAGGATTACATTTGTTCTCCTCATGCTGAAAGTATGAGGAAGA
  GAAACCAGATTGTGTTCACCATGGTGGAGGCAGAGTCAGAGTACGTTCACCAGCTCTACA
  TCCTGGTCAATGGCTTTCTCCGGCCCCTGCGTATGGCCGCCAGCTCCAAGAAGCCCCCCA
  TCAGCCACGACGACGTCAGCAGTATTTTTCTTAACAGTGAAACAATCATGTTTCTTCATG
  AAATATTTCATCAAGGACTAAAGGCAAGGATAGCAAACTGGCCCACTTTAATTTTAGCTG
  ATCTGTTTGATATTTTGCTCCCCATGCTGAACATTTATCAAGAATTTGTGCGTAATCACC
  AGTACAGCCTGCAAGTTCTCGCCAATTGTAAGCAAAACAGAGATTTTGACAAACTCTTAA
  AACAGTATGAAGCCAATCCAGCCTGTGAGGGGAGGATGCTGGAGACATTCTTGACCTATC
  CCATGTTTCAGATCCCCAGATATATCATCACACTCCATGAGCTCCTTGCTCACACACCCC
  ATGAGCATGTGGAAAGGAAAAGCCTGGAGTTTGCCAAATCAAAGCTAGAGGAACTATCCA
  GAGTAATGCACGATGAAGTCAGCGACACTGAAAACATAAGGAAAAACCTTGCCATCGAAA
  GAATGATCGTGGAGGGCTGTGACATCTTGCTGGACACCAGCCAAACGTTCATCCGCCAAG
  GTTCTCTTATTCAAGTACCTTCCGTTGAGAGGGGGAAACTTAGTAAAGTTCGCCTGGGTT
  CGTTGTCTTTGAAAAAGGAAGGAGAGAGACAATGCTTCTTATTTACAAAACACTTTTTAA
  TATGTACAAGAAGTTCAGGAGGGAAGCTTCATCTGCTCAAGACAGGTGGGGTTCTGTCTC
  TAATAGACTGCACATTGATTGAGGAGCCAGATGCAAGCGATGATGACTCTAAAGGTTCTG
  GGCAAGTGTTTGGGCACCTGGATTTTAAAATAGTGGTGGAGCCTCCTGACGCTGCCGCCT
  TCACTGTTGTCTTGTTAGCACCCTCACGCCAGGAGAAAGCTGCCTGGATGAGTGACATCA
  GTCAGTGTGTGGACAATATACGATGTAATGGTTTAATGACTATAGTGTTTGAAGAGAATT
  CCAAAGTCACTGTGCCACATATGATTAAGTCTGATGCCCGTCTTCATAAAGACGACACTG
  ACATTTGCTTCAGTAAAACACTCAACTCCTGCAAAGTGCCCCAGATCCGTTATGCCAGCG
  TGGAGCGCCTCTTGGAACGACTGACAGACTTGCGGTTTCTTAGTATTGATTTCCTCAACA
  CCTTTCTGCACACCTATCGTATTTTCACTACTGCCGCTGTGGTGCTGGGGAAACTCTCCG
  ACATATACAAGAGGCCTTTCACCTCCATCCCTGTCAGGTCATTGGAATTGTTTTTTGCTA
  CCAGCCAGAACAACAGAGGTGAACATTTGGTGGATGGCAAATCCCCACGTCTGTGTCGCA
  AATTCTCTTCCCCGCCACCACTGGCTGTGTCCAGAACATCTTCCCCAGTGAGGGCCAGAA
  AGCTGTCTTTGACTTCTCCCTTGAACTCAAAGATAGGAGCATTGGACCTGACAACTTCCA
  GCAGTCCCACCACCACCACCCAGAGTCCCGCTGCGTCTCCACCACCACACACTGGTCAGA
  TACCACTGGATCTCAGCAGAGGCCTCTCTTCTCCAGAGCAAAGCCCGGGAACGGTAGAAG
  AGAATGTCGATAACCCACGCGTGGATCTGTGTAACAAGCTAAAACGAAGTATTCAAAAAG
  CAGTCCTAGAGTCTGCACCAGCGGACCGAGCAGGAGTGGAAAGCTCCCCTGCAGCGGACA
  CCACAGAACTTTCACCTTGCAGATCCCCCTCAACTCCTCGGCACCTCCGCTATCGACAGC
  CTGGAGGACAGACGGCGGACAATGCCCACTGCTCTGTTTCACCGGCTTCTGCTTTTGCAA
  TAGCCACAGCTGCAGCAGGACATGGGAGTCCACCAGGCTTTAACAACACCGAGAGAACAT
  GTGATAAAGAGTTTATTATACGGAGAACGGCTACCAATCGAGTTCTGAACGTCCTCCGTC
  ACTGGGTCTCAAAGCACGCACAGGATTTCGAACTCAACAATGAACTAAAGATGAATGTCC
  TAAATTTGCTAGAAGAAGTTTTGCGAGACCCAGACCTTCTTCCCCAAGAAAGGAAAGCCG
  CCGCGAATATCCTCAGGGCCCTTTCACAAGATGACCAAGATGACATCCACCTAAAATTAG
  AGGATATAATTCAAATGACTGACTGCATGAAGGCCGAATGCTTTGAGTCCTTGTCGGCCA
  TGGAGCTGGCAGAACAGATCACCCTCCTGGACCATGTCATTTTCAGAAGCATTCCCTACG
  AGGAGTTTCTTGGGCAGGGGTGGATGAAGCTGGATAAAAACGAAAGAACTCCTTACATTA
  TGAAAACCAGCCAACACTTCAATGACATGAGTAACCTGGTGGCCTCCCAGATAATGAACT
  ATGCTGATGTCAGCTCCCGTGCCAACGCCATCGAGAAATGGGTGGCAGTGGCGGACATCT
  GCCGATGCCTGCACAACTACAACGGCGTGCTGGAGATCACCTCGGCCTTAAACAGAAGTG
  CCATCTACAGGCTGAAGAAAACCTGGGCCAAGGTCTCTAAGCAGACAAAAGCTCTAATGG
  ACAAACTTCAAAAGACTGTTTCCTCTGAAGGAAGATTTAAAAATCTTAGAGAAACCCTTA
  AAAATTGTAACCCTCCTGCAGTTCCTTATCTTGGGATGTACTTGACAGACCTGGCATTTA
  TTGAAGAAGGAACACCAAACTTTACTGAGGAAGGCCTTGTCAATTTCTCCAAAATGAGAA
  TGATATCACACATCATCAGAGAGATACGCCAGTTCCAGCAGACTTCCTACAGAATAGATC
  ATCAGCCAAAGGTCGCACAGTACTTGCTTGACAAAGACCTTATCATAGATGAAGATACGC
  TATATGAGCTGTCACTAAAAATTGAACCTCGACTCCCTGCTTGAAGATCTGGCCTTGCCC
  CTGAGTCCACGGGATGTTCATGGAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_006909
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_006909.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006909.1, NP_008840.1
• RefSeq Size: 4078 bp
• RefSeq ORF: 3714 bp
• Locus ID: 5924
• Cytogenetics: 5q14.1
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq, Oct 2011]'