UPF2 (NM_015542) Human Untagged Clone

CAT#: SC315325

UPF2 (untagged)-Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UPF2
• Synonyms: HUPF2; RENT2; smg-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015542, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCAGCTGAGCGTAAAAAGCCAGCAAGTATGGAAGAAAAAGACTCTTTACCAAACAACAAGGAAAAAG
  ACTGCAGTGAAAGGCGGACAGTGAGCAGCAAGGAGAGGCCAAAAGACGATATCAAGCTCACTGCCAAGAA
  GGAGGTCAGCAAGGCCCCTGAAGACAAGAAGAAGAGACTGGAAGATGATAAGAGAAAAAAGGAAGACAAG
  GAACGCAAGAAAAAAGACGAAGAAAAGGTGAAGGCAGAGGAAGAATCAAAGAAAAAAGAAGAGGAAGAAA
  AAAAGAAACATCAAGAGGAAGAGAGAAAGAAGCAAGAAGAGCAGGCCAAACGTCAGCAAGAAGAAGAAGC
  AGCTGCTCAGATGAAAGAAAAAGAAGAATCCATTCAGCTTCATCAGGAAGCTTGGGAACGACATCATTTA
  AGAAAGGAACTTCGTAGCAAAAACCAAAATGCTCCGGACAGCCGACCAGAGGAAAACTTCTTCAGCCGCC
  TCGACTCAAGTTTGAAGAAAAATACTGCTTTTGTCAAGAAACTAAAAACTATTACAGAACAACAGAGAGA
  CTCCTTGTCCCATGATTTTAATGGCCTAAATTTAAGCAAATACATTGCAGAAGCTGTAGCTTCCATCGTG
  GAAGCAAAACTAAAAATCTCTGATGTGAACTGTGCTGTGCACCTCTGCTCTCTCTTTCACCAGCGTTATG
  CTGACTTTGCCCCATCACTTCTTCAGGTCTGGAAAAAACATTTTGAAGCAAGGAAAGAGGAGAAAACACC
  TAACATCACCAAGTTAAGAACTGATTTGCGTTTTATTGCAGAATTGACAATAGTTGGGATTTTCACTGAC
  AAGGAAGGTCTTTCCTTAATCTATGAACAGCTAAAAAATATTATTAATGCTGATCGGGAGTCCCACACTC
  ATGTCTCTGTAGTGATTAGTTTCTGTCGACATTGTGGAGATGATATTGCTGGACTTGTACCAAGGAAAGT
  AAAGAGTGCTGCAGAGAAGTTTAATTTGAGTTTTCCTCCTAGTGAGATAATTAGTCCAGAGAAACAACAG
  CCCTTCCAGAATCTTTTAAAAGAGTACTTTACGTCTTTGACCAAACACCTGAAAAGGGACCACAGGGAGC
  TCCAGAATACTGAGAGACAAAACAGGCGCATTCTACATTCTAAAGGGGAGCTCAGTGAAGATAGACATAA
  ACAGTATGAGGAATTTGCTATGTCTTACCAGAAGCTGCTGGCAAATTCTCAATCCTTAGCAGACCTTTTG
  GATGAAAATATGCCAGATCTTCCTCAAGACAAACCAACACCAGAAGAACATGGGCCTGGAATTGATATAT
  TCACACCTGGTAAACCTGGAGAATATGACTTGGAAGGTGGTATATGGGAAGATGAAGATGCTCGGAATTT
  TTATGAGAACCTCATTGATTTGAAGGCTTTTGTCCCAGCCATCTTGTTTAAAGACAATGAAAAAAGTTGT
  CAGAATAAAGAGTCCAACAAAGATGATACCAAAGAGGCAAAAGAATCTAAGGAGAATAAGGAGGTATCAA
  GTCCCGATGATTTGGAACTTGAGTTGGAGAATCTAGAAATTAATGATGACACCTTAGAATTAGAGGGTGG
  AGATGAAGCTGAAGATCTTACAAAGAAACTTCTTGATGAACAAGAACAAGAAGATGAGGAAGCCAGCACT
  GGATCTCATCTCAAGCTCATAGTAGATGCTTTCCTACAGCAGTTACCCAACTGTGTCAACCGAGATCTGA
  TAGACAAGGCAGCAATGGATTTTTGCATGAACATGAACACAAAAGCAAACAGGAAGAAGTTGGTACGGGC
  ACTCTTCATAGTTCCTAGACAAAGGTTGGATTTGCTACCATTTTATGCAAGATTGGTTGCTACATTGCAT
  CCCTGCATGTCTGATGTAGCAGAGGATCTTTGTTCCATGCTGAGGGGGGATTTCAGATTTCATGTACGGA
  AAAAGGACCAGATCAATATTGAAACAAAGAATAAAACTGTTCGTTTTATAGGAGAACTAACTAAGTTTAA
  GATGTTCACCAAAAATGACACACTGCATTGTTTAAAGATGCTTCTGTCAGACTTCTCTCATCACCATATT
  GAAATGGCATGCACCCTGCTGGAGACATGTGGACGGTTTCTTTTCAGATCTCCAGAATCTCACCTGAGGA
  CCAGTGTACTTTTGGAGCAAATGATGAGAAAGAAGCAAGCAATGCATCTTGATGCGAGATACGTCACAAT
  GGTAGAGAATGCATATTACTACTGCAACCCACCTCCAGCTGAAAAAACCGTGAAAAAGAAACGTCCTCCT
  CTCCAGGAATATGTCCGGAAACTTTTGTACAAGGATCTCTCTAAGGTTACCACCGAGAAGGTTTTGAGAC
  AGATGCGAAAGCTGCCCTGGCAGGACCAAGAAGTGAAAGACTATGTTATTTGTTGTATGATAAACATCTG
  GAATGTGAAATATAATAGTATTCATTGTGTAGCCAACCTCTTAGCAGGACTAGTGCTCTACCAAGAGGAT
  GTTGGGATCCACGTTGTGGATGGAGTGTTAGAAGATATTCGATTAGGAATGGAGGTTAATCAACCTAAAT
  TTAATCAGAGGCGCATCAGCAGTGCCAAGTTCTTAGGAGAACTTTACAATTACCGAATGGTGGAATCAGC
  TGTTATTTTCAGAACTCTGTATTCTTTTACCTCATTTGGTGTTAATCCTGATGGCTCTCCAAGTTCCCTG
  GACCCACCTGAGCATCTTTTCAGAATTAGACTCGTATGCACTATTCTGGACACATGTGGCCAGTACTTTG
  ACAGAGGTTCCAGTAAACGAAAACTTGATTGTTTCCTTGTATATTTTCAGCGTTATGTTTGGTGGAAGAA
  AAGTTTGGAGGTTTGGACAAAAGACCATCCATTTCCTATTGATATAGATTACATGATCAGTGATACACTA
  GAACTGCTAAGACCAAAGATCAAACTCTGTAATTCTCTGGAAGAATCCATCAGGCAGGTACAAGACTTGG
  AACGAGAATTCTTAATAAAACTAGGCCTAGTAAATGACAAAGACTCAAAAGATTCTATGACAGAAGGAGA
  AAATCTTGAAGAGGATGAAGAAGAAGAAGAAGGTGGGGCTGAAACAGAAGAACAATCTGGAAATGAAAGT
  GAAGTAAATGAGCCAGAAGAAGAGGAGGGTTCTGATAATGATGATGATGAGGGAGAAGAAGAGGAGGAAG
  AGAATACAGATTACCTTACAGATTCCAATAAGGAAAATGAAACCGATGAAGAGAATACTGAGGTAATGAT
  TAAAGGCGGTGGACTTAAGCATGTACCTTGTGTAGAAGATGAGGACTTCATTCAAGCTCTGGATAAAATG
  ATGCTAGAAAATCTACAGCAACGAAGTGGTGAATCTGTTAAAGTGCACCAACTAGATGTTGCCATTCCTT
  TGCATCTCAAAAGCCAGCTGAGGAAAGGGCCCCCACTGGGAGGTGGGGAAGGAGAGGCTGAGTCTGCAGA
  CACAATGCCGTTTGTCATGTTAACAAGAAAAGGCAATAAACAGCAGTTTAAGATCCTTAATGTACCCATG
  TCCTCTCAACTTGCTGCAAATCACTGGAACCAGCAACAGGCAGAACAAGAAGAGAGGATGAGAATGAAAA
  AGCTCACACTAGATATCAATGAACGGCAAGAACAAGAAGATTATCAAGAAATGTTGCAGTCTCTTGCACA
  GCGCCCAGCTCCAGCAAACACCAATCGTGAGAGGCGGCCTCGCTACCAACATCCGAAGGGAGCACCTAAT
  GCAGATCTAATCTTTAAGACTGGTGGGAGGAGACGTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015542
• ORF Size: 3819 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_015542.3, NP_056357.1
• RefSeq Size: 5217
• RefSeq ORF: 3819
• Locus ID: 26019
• Domains: MIF4G, Upf2
• Gene Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains a different 5' UTR than variant 1 and is the shorter transcript.