MYH (MUTYH) (NM_001048173) Human Untagged Clone

CAT#: SC315437

MUTYH (untagged)-Human mutY homolog (E. coli) (MUTYH), transcript variant gamma3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MUTYH
• Synonyms: MYH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001048173, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGAAGCCACGAGCAGCCGTGGGAAGTGGTCACAGGAAGCAGGCAGCCAGCCAGGAAGGGAGGCAGA
  AGCATGCTAAGAACAACAGTCAGGCCAAGCCTTCTGCCTGTGATGGCCTGGCCAGGCAGCCGGAAGAGGT
  GGTATTGCAGGCCTCTGTCTCCTCATACCATCTATTCAGAGACGTAGCTGAAGTCACAGCCTTCCGAGGG
  AGCCTGCTAAGCTGGTACGACCAAGAGAAACGGGACCTACCATGGAGAAGACGGGCAGAAGATGAGATGG
  ACCTGGACAGGCGGGCATATGCTGTGTGGGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCCACTGT
  GATCAACTACTATACCGGATGGATGCAGAAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCCCTGGAG
  GAGGTGAATCAACTCTGGGCTGGCCTGGGCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGAGCTCGGA
  AGGTGGTAGAGGAGCTAGGGGGCCACATGCCACGTACAGCAGAGACCCTGCAGCAGCTCCTGCCTGGCGT
  GGGGCGCTACACAGCTGGGGCCATTGCCTCTATCGCCTTTGGCCAGGCAACCGGTGTGGTGGATGGCAAC
  GTAGCACGGGTGCTGTGCCGTGTCCGAGCCATTGGTGCTGATCCCAGCAGCACCCTTGTTTCCCAGCAGC
  TCTGGGGTCTAGCCCAGCAGCTGGTGGACCCAGCCCGGCCAGGAGATTTCAACCAAGCAGCCATGGAGCT
  AGGGGCCACAGTGTGTACCCCACAGCGCCCACTGTGCAGCCAGTGCCCTGTGGAGAGCCTGTGCCGGGCA
  CGCCAGAGAGTGGAGCAGGAACAGCTCTTAGCCTCAGGGAGCCTGTCGGGCAGTCCTGACGTGGAGGAGT
  GTGCTCCCAACACTGGACAGTGCCACCTGTGCCTGCCTCCCTCGGAGCCCTGGGACCAGACCCTGGGAGT
  GGTCAACTTCCCCAGAAAGGCCAGCCGCAAGCCCCCCAGGGAGGAGAGCTCTGCCACCTGTGTTCTGGAA
  CAGCCTGGGGCCCTTGGGGCCCAAATTCTGCTGGTGCAGAGGCCCAACTCAGGTCTGCTGGCAGGACTGT
  GGGAGTTCCCGTCCGTGACCTGGGAGCCCTCAGAGCAGCTTCAGCGCAAGGCCCTGCTGCAGGAACTACA
  GCGTTGGGCTGGGCCCCTCCCAGCCACGCACCTCCGGCACCTTGGGGAGGTTGTCCACACCTTCTCTCAC
  ATCAAGCTGACATATCAAGTATATGGGCTGGCCTTGGAAGGGCAGACCCCAGTGACCACCGTACCACCAG
  GTGCTCGCTGGCTGACGCAGGAGGAATTTCACACCGCAGCTGTTTCCACCGCCATGAAAAAGGTTTTCCG
  TGTGTATCAGGGCCAACAGCCAGGGACCTGTATGGGTTCCAAAAGGTCCCAGGTGTCCTCTCCGTGCAGT
  CGGAAAAAGCCCCGCATGGGCCAGCAAGTCCTGGATAATTTCTTTCGGTCTCACATCTCCACTGATGCAC
  ACAGCCTCAACAGTGCAGCCCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048173
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048173.1, NP_001041638.1
• RefSeq Size: 1791 bp
• RefSeq ORF: 1566 bp
• Locus ID: 4595
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair
• Gene Summary: 'This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'
  Transcript Variant: This variant (gamma3) contains a distinct 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant alpha5. These differences cause translation initiation at a downstream start codon and result in a protein (isoform 4) that is shorter than isoform 5. Variants beta3, gamma3 and beta5 encode the same isoform 4, which has been shown to localize to the nucleus.