MYH (MUTYH) (NM_001048172) Human Untagged Clone

CAT#: SC315438

MUTYH (untagged)-Human mutY homolog (E. coli) (MUTYH), transcript variant gamma2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MUTYH
• Synonyms: MYH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001048172, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGAAGCCACGAGCAGCCGTGGGAAGTGGTCACAGGAAGCAGGCAGCCAGCCAGGAAGGGAGGCAGA
  AGCATGCTAAGAACAACAGTCAGGCCAAGCCTTCTGCCTGTGATGCAGGCCTGGCCAGGCAGCCGGAAGA
  GGTGGTATTGCAGGCCTCTGTCTCCTCATACCATCTATTCAGAGACGTAGCTGAAGTCACAGCCTTCCGA
  GGGAGCCTGCTAAGCTGGTACGACCAAGAGAAACGGGACCTACCATGGAGAAGACGGGCAGAAGATGAGA
  TGGACCTGGACAGGCGGGCATATGCTGTGTGGGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCCAC
  TGTGATCAACTACTATACCGGATGGATGCAGAAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCCCTG
  GAGGAGGTGAATCAACTCTGGGCTGGCCTGGGCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGAGCTC
  GGAAGGTGGTAGAGGAGCTAGGGGGCCACATGCCACGTACAGCAGAGACCCTGCAGCAGCTCCTGCCTGG
  CGTGGGGCGCTACACAGCTGGGGCCATTGCCTCTATCGCCTTTGGCCAGGCAACCGGTGTGGTGGATGGC
  AACGTAGCACGGGTGCTGTGCCGTGTCCGAGCCATTGGTGCTGATCCCAGCAGCACCCTTGTTTCCCAGC
  AGCTCTGGGGTCTAGCCCAGCAGCTGGTGGACCCAGCCCGGCCAGGAGATTTCAACCAAGCAGCCATGGA
  GCTAGGGGCCACAGTGTGTACCCCACAGCGCCCACTGTGCAGCCAGTGCCCTGTGGAGAGCCTGTGCCGG
  GCACGCCAGAGAGTGGAGCAGGAACAGCTCTTAGCCTCAGGGAGCCTGTCGGGCAGTCCTGACGTGGAGG
  AGTGTGCTCCCAACACTGGACAGTGCCACCTGTGCCTGCCTCCCTCGGAGCCCTGGGACCAGACCCTGGG
  AGTGGTCAACTTCCCCAGAAAGGCCAGCCGCAAGCCCCCCAGGGAGGAGAGCTCTGCCACCTGTGTTCTG
  GAACAGCCTGGGGCCCTTGGGGCCCAAATTCTGCTGGTGCAGAGGCCCAACTCAGGTCTGCTGGCAGGAC
  TGTGGGAGTTCCCGTCCGTGACCTGGGAGCCCTCAGAGCAGCTTCAGCGCAAGGCCCTGCTGCAGGAACT
  ACAGCGTTGGGCTGGGCCCCTCCCAGCCACGCACCTCCGGCACCTTGGGGAGGTTGTCCACACCTTCTCT
  CACATCAAGCTGACATATCAAGTATATGGGCTGGCCTTGGAAGGGCAGACCCCAGTGACCACCGTACCAC
  CAGGTGCTCGCTGGCTGACGCAGGAGGAATTTCACACCGCAGCTGTTTCCACCGCCATGAAAAAGGTTTT
  CCGTGTGTATCAGGGCCAACAGCCAGGGACCTGTATGGGTTCCAAAAGGTCCCAGGTGTCCTCTCCGTGC
  AGTCGGAAAAAGCCCCGCATGGGCCAGCAAGTCCTGGATAATTTCTTTCGGTCTCACATCTCCACTGATG
  CACACAGCCTCAACAGTGCAGCCCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001048172
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048172.1, NP_001041637.1
• RefSeq Size: 1794 bp
• RefSeq ORF: 1569 bp
• Locus ID: 4595
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair
• Gene Summary: 'This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'
  Transcript Variant: This variant (gamma2) contains a distinct 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant alpha5. These differences cause translation initiation at a downstream start codon and result in a protein (isoform 3) that is shorter than isoform 5.