MYH (MUTYH) (NM_001048172) Human Untagged Clone

CAT#: SC315438

MUTYH (untagged)-Human mutY homolog (E. coli) (MUTYH), transcript variant gamma2


  "NM_001048172" in other vectors (4)

Reconstitution Protocol

USD 880.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MUTYH
Synonyms MYH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001048172, the custom clone sequence may differ by one or more nucleotides


ATGAGGAAGCCACGAGCAGCCGTGGGAAGTGGTCACAGGAAGCAGGCAGCCAGCCAGGAAGGGAGGCAGA
AGCATGCTAAGAACAACAGTCAGGCCAAGCCTTCTGCCTGTGATGCAGGCCTGGCCAGGCAGCCGGAAGA
GGTGGTATTGCAGGCCTCTGTCTCCTCATACCATCTATTCAGAGACGTAGCTGAAGTCACAGCCTTCCGA
GGGAGCCTGCTAAGCTGGTACGACCAAGAGAAACGGGACCTACCATGGAGAAGACGGGCAGAAGATGAGA
TGGACCTGGACAGGCGGGCATATGCTGTGTGGGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCCAC
TGTGATCAACTACTATACCGGATGGATGCAGAAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCCCTG
GAGGAGGTGAATCAACTCTGGGCTGGCCTGGGCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGAGCTC
GGAAGGTGGTAGAGGAGCTAGGGGGCCACATGCCACGTACAGCAGAGACCCTGCAGCAGCTCCTGCCTGG
CGTGGGGCGCTACACAGCTGGGGCCATTGCCTCTATCGCCTTTGGCCAGGCAACCGGTGTGGTGGATGGC
AACGTAGCACGGGTGCTGTGCCGTGTCCGAGCCATTGGTGCTGATCCCAGCAGCACCCTTGTTTCCCAGC
AGCTCTGGGGTCTAGCCCAGCAGCTGGTGGACCCAGCCCGGCCAGGAGATTTCAACCAAGCAGCCATGGA
GCTAGGGGCCACAGTGTGTACCCCACAGCGCCCACTGTGCAGCCAGTGCCCTGTGGAGAGCCTGTGCCGG
GCACGCCAGAGAGTGGAGCAGGAACAGCTCTTAGCCTCAGGGAGCCTGTCGGGCAGTCCTGACGTGGAGG
AGTGTGCTCCCAACACTGGACAGTGCCACCTGTGCCTGCCTCCCTCGGAGCCCTGGGACCAGACCCTGGG
AGTGGTCAACTTCCCCAGAAAGGCCAGCCGCAAGCCCCCCAGGGAGGAGAGCTCTGCCACCTGTGTTCTG
GAACAGCCTGGGGCCCTTGGGGCCCAAATTCTGCTGGTGCAGAGGCCCAACTCAGGTCTGCTGGCAGGAC
TGTGGGAGTTCCCGTCCGTGACCTGGGAGCCCTCAGAGCAGCTTCAGCGCAAGGCCCTGCTGCAGGAACT
ACAGCGTTGGGCTGGGCCCCTCCCAGCCACGCACCTCCGGCACCTTGGGGAGGTTGTCCACACCTTCTCT
CACATCAAGCTGACATATCAAGTATATGGGCTGGCCTTGGAAGGGCAGACCCCAGTGACCACCGTACCAC
CAGGTGCTCGCTGGCTGACGCAGGAGGAATTTCACACCGCAGCTGTTTCCACCGCCATGAAAAAGGTTTT
CCGTGTGTATCAGGGCCAACAGCCAGGGACCTGTATGGGTTCCAAAAGGTCCCAGGTGTCCTCTCCGTGC
AGTCGGAAAAAGCCCCGCATGGGCCAGCAAGTCCTGGATAATTTCTTTCGGTCTCACATCTCCACTGATG
CACACAGCCTCAACAGTGCAGCCCAGTGA


Restriction Sites SgfI-MluI     
ACCN NM_001048172
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001048172.1, NP_001041637.1
RefSeq Size 1794 bp
RefSeq ORF 1569 bp
Locus ID 4595
Cytogenetics 1p34.1
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Base excision repair
Gene Summary 'This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'
Transcript Variant: This variant (gamma2) contains a distinct 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant alpha5. These differences cause translation initiation at a downstream start codon and result in a protein (isoform 3) that is shorter than isoform 5.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.