CD56 (NCAM1) (NM_001076682) Human Untagged Clone

CAT#: SC315450

NCAM1 (untagged)-Human neural cell adhesion molecule 1 (NCAM1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCAM1
• Synonyms: CD56; MSK39; NCAM
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001076682, the custom clone sequence may differ by one or more nucleotides
  ATGCTGCAAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCTCTG
  CAGGTGGATATTGTTCCCAGCCAGGGGGAGATCAGCGTTGGAGAGTCCAAATTCTTCTTA
  TGCCAAGTGGCAGGAGATGCCAAAGATAAAGACATCTCCTGGTTCTCCCCCAATGGAGAA
  AAGCTCACCCCAAACCAGCAGCGGATCTCAGTGGTGTGGAATGATGATTCCTCCTCCACC
  CTCACCATCTATAACGCCAACATCGACGACGCCGGCATTTACAAGTGTGTGGTTACAGGC
  GAGGATGGCAGTGAGTCAGAGGCCACCGTCAACGTGAAGATCTTTCAGAAGCTCATGTTC
  AAGAATGCGCCAACCCCACAGGAGTTCCGGGAGGGGGAAGATGCCGTGATTGTGTGTGAT
  GTGGTCAGCTCCCTCCCACCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATCCTG
  AAAAAAGATGTCCGATTCATAGTCCTGTCCAACAACTACCTGCAGATCCGGGGCATCAAG
  AAAACAGATGAGGGCACTTATCGCTGTGAGGGCAGAATCCTGGCACGGGGGGAGATCAAC
  TTCAAGGACATTCAGGTCATTGTGAATGTGCCACCTACCATCCAGGCCAGGCAGAATATT
  GTGAATGCCACCGCCAACCTCGGCCAGTCCGTCACCCTGGTGTGCGATGCCGAAGGCTTC
  CCAGAGCCCACCATGAGCTGGACAAAGGATGGGGAACAGATAGAGCAAGAGGAAGACGAT
  GAGAAGTACATCTTCAGCGACGATAGTTCCCAGCTGACCATCAAAAAGGTGGATAAGAAC
  GACGAGGCTGAGTACATCTGCATTGCTGAGAACAAGGCTGGCGAGCAGGATGCGACCATC
  CACCTCAAAGTCTTTGCAAAACCCAAAATCACATATGTAGAGAACCAGACTGCCATGGAA
  TTAGAGGAGCAGGTCACTCTTACCTGTGAAGCCTCCGGAGACCCCATTCCCTCCATCACC
  TGGAGGACTTCTACCCGGAACATCAGCAGCGAAGAAAAGACTCTGGATGGGCACATGGTG
  GTGCGTAGCCATGCCCGTGTGTCGTCGCTGACCCTGAAGAGCATCCAGTACACTGATGCC
  GGAGAGTACATCTGCACCGCCAGCAACACCATCGGCCAGGACTCCCAGTCCATGTACCTT
  GAAGTGCAATATGCCCCAAAGCTACAGGGCCCTGTGGCTGTGTACACTTGGGAGGGGAAC
  CAGGTGAACATCACCTGCGAGGTATTTGCCTATCCCAGTGCCACGATCTCATGGTTTCGG
  GATGGCCAGCTGCTGCCAAGCTCCAATTACAGCAATATCAAGATCTACAACACCCCCTCT
  GCCAGCTATCTGGAGGTGACCCCAGACTCTGAGAATGATTTTGGGAACTACAACTGTACT
  GCAGTGAACCGCATTGGGCAGGAGTCCTTGGAATTCATCCTTGTTCAAGCAGACACCCCC
  TCTTCACCATCCATCGACCAGGTGGAGCCATACTCCAGCACAGCCCAGGTGCAGTTTGAT
  GAACCAGAGGCCACAGGTGGGGTGCCCATCCTCAAATACAAAGCTGAGTGGAGAGCAGTT
  GGTGAAGAAGTATGGCATTCCAAGTGGTATGATGCCAAGGAAGCCAGCATGGAGGGCATC
  GTCACCATCGTGGGCCTGAAGCCCGAAACAACGTACGCCGTAAGGCTGGCGGCGCTCAAT
  GGCAAAGGGCTGGGTGAGATCAGCGCGGCCTCCGAGTTCAAGACGCAGCCAGTCCATAGC
  CCTCCTCCACCGGCATCTGCTAGCTCGTCTACCCCTGTTCCATTGTCTCCACCAGATACA
  ACTTGGCCTCTTCCTGCCCTTGCAACCACAGAACCAGCTAAAGGGGAACCCAGTGCACCT
  AAGCTCGAAGGGCAGATGGGAGAGGATGGAAACTCTATTAAAGTGAACCTGATCAAGCAG
  GATGACGGCGGCTCCCCCATCAGACACTATCTGGTCAGGTACCGAGCGCTCTCCTCCGAG
  TGGAAACCAGAGATCAGGCTCCCGTCTGGCAGTGACCACGTCATGCTGAAGTCCCTGGAC
  TGGAATGCTGAGTATGAGGTCTACGTGGTGGCTGAGAACCAGCAAGGAAAATCCAAGGCG
  GCTCATTTTGTGTTCAGGACCTCGGCCCAGCCCACAGCCATCCCAGCAACCTTGGGAGGC
  AATTCTGCATCCTACACCTTTGTCTCATTGCTTTTCTCTGCAGTGACTCTTCTTTTGCTC
  TGT
  
• Restriction Sites: Please inquire
• ACCN: NM_001076682
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001076682.1, NP_001070150.1
• RefSeq Size: 4913 bp
• RefSeq ORF: 2286 bp
• Locus ID: 4684
• Cytogenetics: 11q23.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Prion diseases
• Gene Summary: 'This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (3) has multiple differences in the coding region and uses an alternate splice site in the 3' coding region compared to variant 5. The resulting protein (isoform 3) is shorter and has a distinct C-terminus compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.