TrkA (NTRK1) (NM_002529) Human Untagged Clone

CAT#: SC315454

NTRK1 (untagged)-Human neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NTRK1
• Synonyms: MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002529 edited
  ATGCTGCGAGGCGGACGGCGCGGGCAGCTTGGCTGGCACAGCTGGGCTGCGGGGCCGGGC
  AGCCTGCTGGCTTGGCTGATACTGGCATCTGCGGGCGCCGCACCCTGCCCCGATGCCTGC
  TGCCCCCACGGCTCCTCGGGACTGCGATGCACCCGGGATGGGGCCCTGGATAGCCTCCAC
  CACCTGCCCGGCGCAGAGAACCTGACTGAGCTCTACATCGAGAACCAGCAGCATCTGCAG
  CATCTGGAGCTCCGTGATCTGAGGGGCCTGGGGGAGCTGAGAAACCTCACCATCGTGAAG
  AGTGGTCTCCGTTTCGTGGCGCCAGATGCCTTCCATTTCACTCCTCGGCTCAGTCGCCTG
  AATCTCTCCTTCAACGCTCTGGAGTCTCTCTCCTGGAAAACTGTGCAGGGCCTCTCCTTA
  CAGGAACTGGTCCTGTCGGGGAACCCTCTGCACTGTTCTTGTGCCCTGCGCTGGCTACAG
  CGCTGGGAGGAGGAGGGACTGGGCGGAGTGCCTGAACAGAAGCTGCAGTGTCATGGGCAA
  GGGCCCCTGGCCCACATGCCCAATGCCAGCTGTGGTGTGCCCACGCTGAAGGTCCAGGTG
  CCCAATGCCTCGGTGGATGTGGGGGACGACGTGCTGCTGCGGTGCCAGGTGGAGGGGCGG
  GGCCTGGAGCAGGCCGGCTGGATCCTCACAGAGCTGGAGCAGTCAGCCACGGTGATGAAA
  TCTGGGGGTCTGCCATCCCTGGGGCTGACCCTGGCCAATGTCACCAGTGACCTCAACAGG
  AAGAACGTGACGTGCTGGGCAGAGAACGATGTGGGCCGGGCAGAGGTCTCTGTTCAGGTC
  AACGTCTCCTTCCCGGCCAGTGTGCAGCTGCACACGGCGGTGGAGATGCACCACTGGTGC
  ATCCCCTTCTCTGTGGATGGGCAGCCGGCACCGTCTCTGCGCTGGCTCTTCAATGGCTCC
  GTGCTCAATGAGACCAGCTTCATCTTCACTGAGTTCCTGGAGCCCGCAGCCAATGAGACC
  GTGCGGCACGGGTGTCTGCGCCTCAACCAGCCCACCCACGTCAACAACGGCAACTACACG
  CTGCTGGCTGCCAACCCCTTCGGCCAGGCCTCCGCCTCCATCATGGCTGCCTTCATGGAC
  AACCCTTTCGAGTTCAACCCCGAGGACCCCATCCCTGTCTCCTTCTCGCCGGTGGACACT
  AACAGCACATCTGGAGACCCGGTGGAGAAGAAGGACGAAACACCTTTTGGGGTCTCGGTG
  GCTGTGGGCCTGGCCGTCTTTGCCTGCCTCTTCCTTTCTACGCTGCTCCTTGTGCTCAAC
  AAATGTGGACGGAGAAACAAGTTTGGGATCAACCGCCCGGCTGTGCTGGCTCCAGAGGAT
  GGGCTGGCCATGTCCCTGCATTTCATGACATTGGGTGGCAGCTCCCTGTCCCCCACCGAG
  GGCAAAGGCTCTGGGCTCCAAGGCCACATCATCGAGAACCCACAATACTTCAGTGATGCC
  TGTGTTCACCACATCAAGCGCCGGGACATCGTGCTCAAGTGGGAGCTGGGGGAGGGCGCC
  TTTGGGAAGGTCTTCCTTGCTGAGTGCCACAACCTCCTGCCTGAGCAGGACAAGATGCTG
  GTGGCTGTCAAGGCACTGAAGGAGGCGTCCGAGAGTGCTCGGCAGGACTTCCAGCGTGAG
  GCTGAGCTGCTCACCATGCTGCAGCACCAGCACATCGTGCGCTTCTTCGGCGTCTGCACC
  GAGGGCCGCCCCCTGCTCATGGTCTTTGAGTATATGCGGCACGGGGACCTCAACCGCTTC
  CTCCGATCCCATGGACCTGATGCCAAGCTGCTGGCTGGTGGGGAGGATGTGGCTCCAGGC
  CCCCTGGGTCTGGGGCAGCTGCTGGCCGTGGCTAGCCAGGTCGCTGCGGGGATGGTGTAC
  CTGGCGGGTCTGCATTTTGTGCACCGGGACCTGGCCACACGCAACTGTCTAGTGGGCCAG
  GGACTGGTGGTCAAGATTGGTGATTTTGGCATGAGCAGGGATATCTACAGCACCGACTAT
  TACCGTGTGGGAGGCCGCACCATGCTGCCCATTCGCTGGATGCCGCCCGAGAGCATCCTG
  TACCGTAAGTTCACCACCGAGAGCGACGTGTGGAGCTTCGGCGTGGTGCTCTGGGAGATC
  TTCACCTACGGCAAGCAGCCCTGGTACCAGCTCTCCAACACGGAGGCAATCGACTGCATC
  ACGCAGGGACGTGAGTTGGAGCGGCCACGTGCCTGCCCACCAGAGGTCTACGCCATCATG
  CGGGGCTGCTGGCAGCGGGAGCCCCAGCAACGCCACAGCATCAAGGATGTGCACGCCCGG
  CTGCAAGCCCTGGCCCAGGCACCTCCTGTCTACCTGGATGTCCTGGGCTAG
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_002529 unedited
  GGGGNCCCACTACGTTCGTGCCCCCACTNCCACCGNAGNNCATTGGGCACCTGGNACCTT
  CAGCGTGGGCACACCACAGCTGGCATTGGGCATGTGGGCCAGGGGCCCTTGCCCATGACA
  CTGCAGCTTCTGTTCAGGCACTCCGCCCAGTCCCTCCTCCTCCCAGCGCTGTAGCCAGCG
  CAGGGCACAAGAACAGTGCAGAGGGTTCCCCGACAGGACCAGTTCCTGTAAGGAGAGGCC
  CTGCACAGTTTTCCAGGAGAGAGACTCCAGAGCGTTGAAGGAGAGATTCAGGCGACTGAG
  CCGAGGAGTGAAATGGAAGGCATCTGGCGCCACGAAACGGAGACCACTCTTCACGATGGT
  GAGGTTTCTCAGCTCCCCCAGGCCCCTCAGATCACGGAGCTCCAGATGCTGCAGATGCTG
  CTGGTTCTCGATGTAGAGCTCAGTCAGGTTCTCTGCGCCGGGCAGGTGGTGGAGGCTATC
  CAGGGCCCCATCCCGGGTGCATCGCAGTCCCGAGGAGCCGTGGGGGCAGCAGGCATCGGG
  GCAGGGTGCGGCGCCCGCAGATGCCAGTATCAGCCAAGCCAGCAGGCTGCCCGGCCCCGC
  AGCCCAGCTGTGCCAGCCAAGCTGCCCGCGCCGTCCGCCTCGCAGCATGCGGCCGCCCTA
  TAGTGAGTCGTATTACANAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCT
  CCCACCGTACACGCCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTT
  GGGAAAGTCCCCGTGATTTTTGGTGCCAAAACAAACTNCCATTGGACGTCATGGGTTGGA
  GACTTGGAAATCCCCGTGAGTCAAACCGCTATCCAGCCCCATTGGTGTACTGCCCAAACC
  GATCAACCTGGTAATAGCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002529 unedited
  GGCAATGGGGAGGCAACTTCCAGGCCAGNATAGCACTGGGGAGGGTACAGGGATGCCCCC
  GGGACGTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGAGCGTCACGAGCTAGCCCAG
  GACATCCAGGTAGACAGGAGGTGCCTGGGCCAGGGCTTGCAGCCGGGCGTGCACATCCTT
  GATGCTGTGGCGTTGCTGGGGCTCCCGCTGCCAGCAGCCCCGCATGATGGCGTAGACCTC
  TGGTGGGCAGGCACGTGGCCGCTCCAACTCACGTCCCTGCGTGATGCAGTCGATTGCCTC
  CGTGTTGGAGAGCTGGTACCAGGGCTGCTTGCCGTAGGTGAAGATCTCCCAGAGCACCAC
  GCCGAAGCTCCACACGTCGCTCTCGGTGGTGAACTTACGGTACAGGATGCTCTCGGGCGG
  CATCCAGCGAATGGGCAGCATGGTGCGGCCTCCCACACGGTAATAGTCGGTGCTGTAGAT
  ATCCCTGCTCATGCCAAAATCACCAATCTTGACCCACCAGTCCCTGNCCCACTAGACAGT
  TTGCGTGTGGCCAGGTCCCGGTGCACANAATGCAGACCCGCCAGGTACACCATCCCCGCA
  GCGACCTGGCTAGCCANCGGGGGGCNAGCACCTGCCCAAACCCAGGGGGCCTGGACCCCC
  ATTCTCCCCACAACCAAAACTTGGAATTAGTCCATGGAATCNNNNNAAGAAAAAAAAACC
  CCTTTGTAGTCCCCTCTGGCCGCCATTTCTCTAAAAACTATAGCCGGGGGGGGCCCCTCT
  TGGGCCAACCCCCCAAAAACCCCCAAGTGTGTTGGTTTTCCCCCGTGGGAAAAACCCCCA
  CCCCCCCCCCGGAAAATCCTGGGAAAAACTTTTGGGACCCTCTTTTTTTTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_002529
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002529.3, NP_002520.2
• RefSeq Size: 2663 bp
• RefSeq ORF: 2391 bp
• Locus ID: 4914
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Apoptosis, Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Thyroid cancer
• Gene Summary: 'This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).