Nephrin (NPHS1) (NM_004646) Human Untagged Clone

CAT#: SC315460

NPHS1 (untagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPHS1
• Synonyms: CNF; nephrin; NPHN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004646 edited
  GCCACCATGGCCCTGGGGACGACGCTCAGGGCTTCTCTCCTGCTCCTGGGGCTGCTGACT
  GAAGGCCTGGCGCAGTTGGCGATTCCTGCCTCCGTTCCCCGGGGCTTCTGGGCCCTGCCT
  GAAAACCTGACGGTGGTGGAGGGGGCCTCAGTGGAGCTGCGTTGTGGGGTCAGCACCCCT
  GGCAGTGCGGTGCAATGGGCCAAAGATGGGCTGCTCCTGGGCCCCGACCCCAGGATCCCA
  GGCTTCCCGAGGTACCGCCTGGAAGGGGACCCTGCTAGAGGTGAATTCCACCTGCACATC
  GAGGCCTGTGACCTCAGCGATGACGCGGAGTATGAGTGCCAGGTCGGCCGCTCTGAGATG
  GGGCCCGAGCTCGTGTCTCCCAGAGTGATCCTCTCCATCCTGGTTCCTCCCAAGCTGCTC
  CTGCTGACCCCAGAGGCAGGCACCATGGTCACCTGGGTAGCTGGGCAGGAGTACGTGGTC
  AACTGTGTGTCTGGGGACGCGAAGCCAGCACCTGACATCACCATTCTCCTGAGTGGACAG
  ACAATATCTGACATCTCTGCAAACGTGAACGAGGGCTCCCAGCAGAAACTCTTCACTGTG
  GAGGCCACAGCCAGGGTGACACCCCGGAGCTCAGATAATAGGCAGTTGCTGGTCTGTGAG
  GCGTCTAGCCCAGCACTGGAGGCCCCCATCAAGGCCTCATTCACCGTGAATGTTCTGTTC
  CCTCCAGGACCCCCTGTCATCGAGTGGCCAGGCCTGGATGAGGGGCACGTGCGGGCAGGA
  CAGAGCTTGGAGCTGCCGTGCGTGGCCCGAGGGGGTAATCCCTTAGCCACACTGCAGTGG
  CTGAAGAATGGCCAGCCGGTGTCCACAGCGTGGGGCACAGAGCACACCCAGGCGGTGGCC
  CGCAGTGTGCTGGTGATGACCGTGAGGCCAGAAGACCATGGAGCGCAGCTCAGCTGCGAG
  GCCCACAACAGCGTGTCTGCAGGGACCCAGGAGCACGGCATCACACTGCAGGTCACCTTT
  CCCCCTAGTGCCATTATTATCTTGGGATCTGCATCCCAGACTGAGAACAAGAACGTGACA
  CTCTCCTGTGTCAGCAAGTCCAGTCGCCCGCGGGTTCTGCTACGATGGTGGCTGGGCTGG
  CGGCAGCTGCTGCCCATGGAGGAGACAGTCATGGATGGACTGCATGGCGGTCACATCTCC
  ATGTCCAACCTGACATTCCTGGCGCGGCGGGAGGACAACGGTCTGACCCTCACATGTGAG
  GCCTTCAGTGAAGCCTTCACCAAGGAGACCTTCAAGAAGTCGCTCATCCTGAACGTAAAA
  TATCCCGCCCAGAAACTGTGGATTGAGGGTCCCCCAGAGGGCCAGAAGCTCCGGGCTGGG
  ACCCGGGTGAGGCTGGTGTGTTTGGCTATCGGGGGCAACCCAGAGCCCTCCCTCATGTGG
  TACAAGGACTCGCGCACCGTGACCGAGTCGCGGCTGCCGCAGGAGTCGCGGCGCGTGCAT
  CTCGGCAGCGTGGAGAAATCTGGGAGCACCTTCTCCCGAGAGCTGGTGCTGGTCACAGGG
  CCGTCGGACAACCAGGCCAAGTTCACGTGCAAGGCTGGACAGCTCAGCGCGTCCACGCAG
  CTGGCGGTGCAGTTTCCCCCAACTAACGTGACGATCCTGGCCAACGCATCCGCACTGCGC
  CCGGGAGACGCCTTAAACTTGACATGCGTCAGCGTCAGCAGCAATCCGCCGGTCAACTTG
  TCCTGGGACAAGGAAGGGGAGAGGCTGGAGGGCGTGGCCGCCCCACCCCGGAGAGCCCCA
  TTCAAAGGCTCCGCCGCCGCCAGGAGCGTCCTTCTGCAAGTGTCATCCCGCGATCATGGC
  CAGCGCGTGACCTGCCGCGCCCACAGCGCCGAGCTCCGCGAAACCGTGAGCTCCTTCTAT
  CGCCTCAACGTACTGTACCGTCCAGAGTTCCTGGGGGAGCAGGTGCTGGTGGTGACCGCG
  GTGGAGCAGGGCGAGGCGTTGCTGCCCGTGTCCGTGTCCGCTAACCCCGCCCCCGAGGCC
  TTCAACTGGACCTTCCGCGGCTATCGCCTCAGTCCAGCGGGCGGCCCCCGGCATCGCATC
  CTGTCCAGCGGGGCTCTGCATCTGTGGAATGTGACCCGCGCGGACGACGGCCTCTATCAG
  CTGCACTGCCAGAACTCTGAGGGCACCGCGGAAGCGCGGCTGCGGCTGGACGTGCACTAT
  GCTCCCACCATCCGTGCCCTCCAGGACCCCACTGAGGTGAACGTCGGGGGTTCTGTGGAC
  ATAGTCTGCACTGTCGATGCCAATCCCATCCTCCCGGGCATGTTCAACTGGGAGAGACTG
  GGAGAAGATGAGGAGGACCAGAGCCTGGATGACATGGAGAAGATATCCAGGGGACCAACG
  GGGCGCCTGCGGATTCACCATGCCAAACTGGCCCAGGCTGGCGCTTACCAGTGCATTGTG
  GACAATGGGGTGGCGCCTCCAGCACGACGGCTGCTCCGTCTTGTTGTCAGATTTGCCCCC
  CAGGTGGAGCACCCCACTCCCCTAACTAAGGTGGCTGCAGCTGGAGACAGCACCAGTTCT
  GCCACCCTCCACTGCCGTGCCCGAGGTGTCCCCAACATCGTTTTCACTTGGACAAAAAAC
  GGGGTCCCTCTGGATCTCCAAGATCCCAGGTACACGGAGCACACATACCACCAGGGTGGT
  GTCCACAGCAGCCTCCTGACCATTGCCAACGTGTCTGCCGCCCAGGATTACGCCCTCTTC
  ACATGTACAGCCACCAACGCCCTTGGCTCGGACCAAACCAACATTCAACTTGTCAGCATC
  AGCCGCCCTGACCCTCCATCAGGATTAAAGGTTGTGAGTCTGACCCCACACTCCGTGGGG
  CTGGAGTGGAAGCCTGGCTTTGATGGGGGCCTGCCACAGAGGTTCTGCATCAGGTATGAG
  GCCCTGGGGACTCCAGGGTTCCACTATGTGGATGTCGTACCACCCCAGGCCACCACCTTC
  ACGCTGACTGGTCTACAGCCTTCTACAAGATACAGGGTCTGGCTGCTGGCCAGTAATGCC
  TTGGGGGACAGTGGACTGGCTGACAAAGGGACCCAGCTTCCCATCACTACCCCAGGTCTC
  CACCAGCCTTCTGGAGAACCTGAAGACCAGCTGCCCACAGAGCCACCTTCAGGACCCTCG
  GGGCTGCCCCTGCTGCCTGTGCTGTTCGCTCTTGGGGGGCTTCTGCTCCTCTCCAATGCC
  TCCTGTGTCGGGGGGGTCCTCTGGCAGCGGAGACTCAGGCGTCTTGCTGAGGGCATCTCA
  GAGAAGACAGAGGCAGGGTCGGAAGAGGACCGAGTCAGGAACGAATATGAGGAGAGCCAG
  TGGACAGGAGAGCGGGACACTCAGAGCTCCACGGTCAGCACAACAGAGGCAGAGCCGTAT
  TACCGCTCCCTGAGGGACTTCAGCCCCCAGCTGCCCCCGACGCAGGAGGAGGTGTCTTAT
  TCCCGAGGTTTCACAGGTGAAGATGAGGATATGGCCTTCCCTGGGCACTTGTATGATGAG
  GTAGAAAGAACGTACCCCCCGTCTGGAGCCTGGGGACCCCTCTACGATGAAGTGCAGATG
  GGACCCTGGGACCTCCACTGGCCTGAAGACACATATCAGGATCCAAGAGGAATCTATGAC
  CAGGTGGCCGGAGACTTGGACACTCTGGAACCCGATTCTCTGCCCTTCGAGCTGAGGGGA
  CATCTGGTGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004646
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004646.1, NP_004637.1
• RefSeq Size: 4285 bp
• RefSeq ORF: 3726 bp
• Locus ID: 4868
• Cytogenetics: 19q13.12
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]'