Nephrin (NPHS1) (NM_004646) Human Untagged Clone

CAT#: SC315460

NPHS1 (untagged)-Human nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)


  "NM_004646" in other vectors (6)

Reconstitution Protocol

USD 2,520.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NPHS1
Synonyms CNF; nephrin; NPHN
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_004646 edited
GCCACCATGGCCCTGGGGACGACGCTCAGGGCTTCTCTCCTGCTCCTGGGGCTGCTGACT
GAAGGCCTGGCGCAGTTGGCGATTCCTGCCTCCGTTCCCCGGGGCTTCTGGGCCCTGCCT
GAAAACCTGACGGTGGTGGAGGGGGCCTCAGTGGAGCTGCGTTGTGGGGTCAGCACCCCT
GGCAGTGCGGTGCAATGGGCCAAAGATGGGCTGCTCCTGGGCCCCGACCCCAGGATCCCA
GGCTTCCCGAGGTACCGCCTGGAAGGGGACCCTGCTAGAGGTGAATTCCACCTGCACATC
GAGGCCTGTGACCTCAGCGATGACGCGGAGTATGAGTGCCAGGTCGGCCGCTCTGAGATG
GGGCCCGAGCTCGTGTCTCCCAGAGTGATCCTCTCCATCCTGGTTCCTCCCAAGCTGCTC
CTGCTGACCCCAGAGGCAGGCACCATGGTCACCTGGGTAGCTGGGCAGGAGTACGTGGTC
AACTGTGTGTCTGGGGACGCGAAGCCAGCACCTGACATCACCATTCTCCTGAGTGGACAG
ACAATATCTGACATCTCTGCAAACGTGAACGAGGGCTCCCAGCAGAAACTCTTCACTGTG
GAGGCCACAGCCAGGGTGACACCCCGGAGCTCAGATAATAGGCAGTTGCTGGTCTGTGAG
GCGTCTAGCCCAGCACTGGAGGCCCCCATCAAGGCCTCATTCACCGTGAATGTTCTGTTC
CCTCCAGGACCCCCTGTCATCGAGTGGCCAGGCCTGGATGAGGGGCACGTGCGGGCAGGA
CAGAGCTTGGAGCTGCCGTGCGTGGCCCGAGGGGGTAATCCCTTAGCCACACTGCAGTGG
CTGAAGAATGGCCAGCCGGTGTCCACAGCGTGGGGCACAGAGCACACCCAGGCGGTGGCC
CGCAGTGTGCTGGTGATGACCGTGAGGCCAGAAGACCATGGAGCGCAGCTCAGCTGCGAG
GCCCACAACAGCGTGTCTGCAGGGACCCAGGAGCACGGCATCACACTGCAGGTCACCTTT
CCCCCTAGTGCCATTATTATCTTGGGATCTGCATCCCAGACTGAGAACAAGAACGTGACA
CTCTCCTGTGTCAGCAAGTCCAGTCGCCCGCGGGTTCTGCTACGATGGTGGCTGGGCTGG
CGGCAGCTGCTGCCCATGGAGGAGACAGTCATGGATGGACTGCATGGCGGTCACATCTCC
ATGTCCAACCTGACATTCCTGGCGCGGCGGGAGGACAACGGTCTGACCCTCACATGTGAG
GCCTTCAGTGAAGCCTTCACCAAGGAGACCTTCAAGAAGTCGCTCATCCTGAACGTAAAA
TATCCCGCCCAGAAACTGTGGATTGAGGGTCCCCCAGAGGGCCAGAAGCTCCGGGCTGGG
ACCCGGGTGAGGCTGGTGTGTTTGGCTATCGGGGGCAACCCAGAGCCCTCCCTCATGTGG
TACAAGGACTCGCGCACCGTGACCGAGTCGCGGCTGCCGCAGGAGTCGCGGCGCGTGCAT
CTCGGCAGCGTGGAGAAATCTGGGAGCACCTTCTCCCGAGAGCTGGTGCTGGTCACAGGG
CCGTCGGACAACCAGGCCAAGTTCACGTGCAAGGCTGGACAGCTCAGCGCGTCCACGCAG
CTGGCGGTGCAGTTTCCCCCAACTAACGTGACGATCCTGGCCAACGCATCCGCACTGCGC
CCGGGAGACGCCTTAAACTTGACATGCGTCAGCGTCAGCAGCAATCCGCCGGTCAACTTG
TCCTGGGACAAGGAAGGGGAGAGGCTGGAGGGCGTGGCCGCCCCACCCCGGAGAGCCCCA
TTCAAAGGCTCCGCCGCCGCCAGGAGCGTCCTTCTGCAAGTGTCATCCCGCGATCATGGC
CAGCGCGTGACCTGCCGCGCCCACAGCGCCGAGCTCCGCGAAACCGTGAGCTCCTTCTAT
CGCCTCAACGTACTGTACCGTCCAGAGTTCCTGGGGGAGCAGGTGCTGGTGGTGACCGCG
GTGGAGCAGGGCGAGGCGTTGCTGCCCGTGTCCGTGTCCGCTAACCCCGCCCCCGAGGCC
TTCAACTGGACCTTCCGCGGCTATCGCCTCAGTCCAGCGGGCGGCCCCCGGCATCGCATC
CTGTCCAGCGGGGCTCTGCATCTGTGGAATGTGACCCGCGCGGACGACGGCCTCTATCAG
CTGCACTGCCAGAACTCTGAGGGCACCGCGGAAGCGCGGCTGCGGCTGGACGTGCACTAT
GCTCCCACCATCCGTGCCCTCCAGGACCCCACTGAGGTGAACGTCGGGGGTTCTGTGGAC
ATAGTCTGCACTGTCGATGCCAATCCCATCCTCCCGGGCATGTTCAACTGGGAGAGACTG
GGAGAAGATGAGGAGGACCAGAGCCTGGATGACATGGAGAAGATATCCAGGGGACCAACG
GGGCGCCTGCGGATTCACCATGCCAAACTGGCCCAGGCTGGCGCTTACCAGTGCATTGTG
GACAATGGGGTGGCGCCTCCAGCACGACGGCTGCTCCGTCTTGTTGTCAGATTTGCCCCC
CAGGTGGAGCACCCCACTCCCCTAACTAAGGTGGCTGCAGCTGGAGACAGCACCAGTTCT
GCCACCCTCCACTGCCGTGCCCGAGGTGTCCCCAACATCGTTTTCACTTGGACAAAAAAC
GGGGTCCCTCTGGATCTCCAAGATCCCAGGTACACGGAGCACACATACCACCAGGGTGGT
GTCCACAGCAGCCTCCTGACCATTGCCAACGTGTCTGCCGCCCAGGATTACGCCCTCTTC
ACATGTACAGCCACCAACGCCCTTGGCTCGGACCAAACCAACATTCAACTTGTCAGCATC
AGCCGCCCTGACCCTCCATCAGGATTAAAGGTTGTGAGTCTGACCCCACACTCCGTGGGG
CTGGAGTGGAAGCCTGGCTTTGATGGGGGCCTGCCACAGAGGTTCTGCATCAGGTATGAG
GCCCTGGGGACTCCAGGGTTCCACTATGTGGATGTCGTACCACCCCAGGCCACCACCTTC
ACGCTGACTGGTCTACAGCCTTCTACAAGATACAGGGTCTGGCTGCTGGCCAGTAATGCC
TTGGGGGACAGTGGACTGGCTGACAAAGGGACCCAGCTTCCCATCACTACCCCAGGTCTC
CACCAGCCTTCTGGAGAACCTGAAGACCAGCTGCCCACAGAGCCACCTTCAGGACCCTCG
GGGCTGCCCCTGCTGCCTGTGCTGTTCGCTCTTGGGGGGCTTCTGCTCCTCTCCAATGCC
TCCTGTGTCGGGGGGGTCCTCTGGCAGCGGAGACTCAGGCGTCTTGCTGAGGGCATCTCA
GAGAAGACAGAGGCAGGGTCGGAAGAGGACCGAGTCAGGAACGAATATGAGGAGAGCCAG
TGGACAGGAGAGCGGGACACTCAGAGCTCCACGGTCAGCACAACAGAGGCAGAGCCGTAT
TACCGCTCCCTGAGGGACTTCAGCCCCCAGCTGCCCCCGACGCAGGAGGAGGTGTCTTAT
TCCCGAGGTTTCACAGGTGAAGATGAGGATATGGCCTTCCCTGGGCACTTGTATGATGAG
GTAGAAAGAACGTACCCCCCGTCTGGAGCCTGGGGACCCCTCTACGATGAAGTGCAGATG
GGACCCTGGGACCTCCACTGGCCTGAAGACACATATCAGGATCCAAGAGGAATCTATGAC
CAGGTGGCCGGAGACTTGGACACTCTGGAACCCGATTCTCTGCCCTTCGAGCTGAGGGGA
CATCTGGTGTAA
Restriction Sites Please inquire     
ACCN NM_004646
Insert Size 3800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004646.1, NP_004637.1
RefSeq Size 4285 bp
RefSeq ORF 3726 bp
Locus ID 4868
Cytogenetics 19q13.12
Protein Families Druggable Genome, Transmembrane
Gene Summary 'This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]'

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