WIPF1 (NM_001077269) Human Untagged Clone

CAT#: SC315577

WIPF1 (untagged)-Human WAS/WASL interacting protein family, member 1 (WIPF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WIPF1
• Synonyms: PRPL-2; WAS2; WASPIP; WIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001077269, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGTCCCTCCCCCTCCAGCACCCCCGCCGCCCCCGACGTTTGCACTGGCCAATACAGAGAAGCCTA
  CCTTGAATAAGACAGAGCAGGCTGGGAGAAATGCTCTCCTTTCTGATATCAGCAAAGGGAAGAAACTAAA
  GAAGACGGTCACCAATGACAGAAGTGCACCAATACTGGACAAACCTAAAGGAGCTGGTGCTGGAGGCGGT
  GGTGGTGGCTTTGGTGGAGGCGGCGGATTTGGCGGAGGAGGTGGTGGCGGAGGCGGTGGAAGTTTTGGAG
  GGGGCGGACCTCCAGGTCTGGGAGGATTGTTCCAGGCTGGAATGCCGAAGCTGAGATCCACGGCCAACAG
  GGATAATGATTCTGGAGGAAGCCGACCACCATTGTTGCCACCGGGAGGAAGATCCACATCTGCGAAACCC
  TTTTCACCCCCAAGTGGCCCAGGGAGGTTTCCTGTGCCTTCTCCAGGCCACAGAAGTGGTCCCCCAGAGC
  CTCAGAGGAACCGAATGCCGCCCCCAAGGCCCGACGTGGGCTCAAAGCCTGATAGCATTCCTCCTCCAGT
  ACCTAGTACTCCAAGACCCATTCAATCAAGTCCGCACAACCGGGGGTCCCCACCAGTGCCCGGAGGCCCC
  AGGCAGCCCAGCCCCGGGCCCACTCCTCCCCCTTTCCCTGGAAACCGCGGCACTGCTTTGGGAGGAGGCT
  CAATACGTCAGTCCCCCTTGAGCTCCTCCTCGCCCTTCTCCAACCGGCCTCCCCTGCCGCCTACCCCCAG
  CAGGGCCTTGGATGACAAACCCCCTCCACCACCTCCTCCAGTGGGCAACAGGCCCTCCATCCACAGGGAA
  GCGGTTCCCCCTCCTCCTCCTCAGAACAACAAGCCTCCAGTGCCTTCCACTCCGCGGCCTTCGGCCTCCT
  CACAGGCCCCACCTCCGCCGCCACCTCCCAGCAGGCCCGGGCCGCCTCCTCTGCCTCCAAGTTCCAGCGG
  CAATGACGAAACCCCAAGACTCCCACAGCGGAATCTGTCCCTCAGTTCGTCCACGCCCCCGTTACCTTCG
  CCAGGACGTTCAGGTCCTCTTCCTCCCCCGCCCAGTGAGAGACCCCCACCTCCAGTGAGGGACCCGCCAG
  GCCGATCAGGCCCCCTCCCACCACCTCCTCCAGTAAGCAGAAACGGCAGCACATCTCGGGCCCTGCCTGC
  TACCCCTCAGTTGCCATCCAGGAGTGGAGTAGACAGTCCCAGGAGTGGACCCAGGCCTCCCCTTCCTCCT
  GATAGGCCCAGTGCTGGGGCACCTCCCCCACCTCCACCATCAACATCTATTAGAAATGGCTTCCAAGACT
  CTCCATGTGAAGATGAGTGGGAAAGCAGATTCTACTTCCATCCGATTTCCGATTTGCCACCTCCAGAGCC
  ATATGTACAAACGACCAAAAGTTATCCCAGCAAACTGGCAAGAAACGAAAGCCGGAGTGGATCCAACCGA
  AGAGAAAGGGGTGCTCCACCACTCCCTCCCATCCCGAGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077269
• Insert Size: 0
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077269.1, NP_001070737.1
• RefSeq Size: 4664 bp
• RefSeq ORF: 1512 bp
• Locus ID: 7456
• Cytogenetics: 2q31.1
• Gene Summary: 'This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.