ketohexokinase (KHK) (NM_000221) Human Untagged Clone

CAT#: SC315989

KHK (untagged)-Human ketohexokinase (fructokinase) (KHK), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KHK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000221 edited
  TCGAGATCGCTTAGCCGCGCTTTAAAAAGGTTTGCATCAGCTGTGAGTCCATCTGACAAG
  CGAGGAAACTAAGGCTGAGAAGTGGGAGGCGTTGCCATCTGCAGGCCCAGGCAACCTGCT
  ACGGGAAGACCGGGGACCAAGACCTCTGGGTTGGCTTTCCTAGACCCGCTCGGGTCTTCG
  GGTGTCGCGAGGAAGGGCCCTGCTCCTTTCGTTCCCTGCACCCCTGGCCGCTGCAGGTGG
  CTCCCTGGAGGAGGAGCTCCCACGCGGAGGAGGAGCCAGGGCAGCTGGGAGCGGGGACAC
  CATCCTCCTGGATAAGAGGCAGAGGCCGGGAGGAACCCCGTCAGCCGGGCGGGCAGGAAG
  CTCTGGGAGTAGCCTCATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTAGTGGTGCTGGA
  CGTCATCAGCCTGGTGGACAAGTACCCTAAGGAGGACTCGGAGATAAGGTGTTTGTCCCA
  GAGATGGCAGCGCGGAGGCAACGCGTCCAACTCCTGCACCATTCTCTCCCTGCTCGGAGC
  CCCCTGTGCCTTCATGGGCTCAATGGCTCCTGGCCATGTTGCTGATTTTGTCCTGGATGA
  CCTCCGCCGTTATTCTGTGGACCTACGCTACACAGTCTTTCAGACCACAGGCTCCGTCCC
  CATCGCCACGGTCATCATCAACGAGGCCAGTGGTAGCCGCACCATCCTATACTATGACAG
  GAGCCTGCCAGATGTGTCTGCTACAGACTTTGAGAAGGTTGATCTGACCCAGTTCAAGTG
  GATCCACATTGAGGGCCGGAACGCATCGGAGCAGGTGAAGATGCTGCAGCGGATAGACGC
  ACACAACACCAGGCAGCCTCCAGAGCAGAAGATCCGGGTGTCCGTGGAGGTGGAGAAGCC
  ACGAGAGGAGCTCTTCCAGCTGTTTGGCTACGGAGACGTGGTGTTTGTCAGCAAAGATGT
  GGCCAAGCACTTGGGGTTCCAGTCAGCAGAGGAAGCCTTGAGGGGCTTGTATGGTCGTGT
  GAGGAAAGGGGCTGTGCTTGTCTGTGCCTGGGCTGAGGAGGGCGCCGACGCCCTGGGCCC
  TGATGGCAAATTGCTCCACTCGGATGCTTTCCCGCCACCCCGCGTGGTGGATACACTGGG
  AGCTGGAGACACCTTCAATGCCTCCGTCATCTTCAGCCTCTCCCAGGGGAGGAGCGTGCA
  GGAAGCACTGAGATTCGGGTGCCAGGTGGCCGGCAAGAAGTGTGGCCTGCAGGGCTTTGA
  TGGCATCGTGTGAGAGCAGGTGCCGGCTCCTCACACACCATGGAGACTACCATTGCGGCT
  GCATCGCCTTCTCCCCTCCATCCAGCCTGGCGTCCAGGTTGCCCTGTTCAGGGGACAGAT
  GCAAGCTGTGGGGAGGACTCTGCCTGTGTCCTGTGTTCCCCACAGGGAGAGGCTCTGGGG
  GGATGGCTGGGGGATGCAGAGCCTCAGAGCAAATAAATCTTCCTCAGAGCCAAAAAAAAA
  AAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000221 unedited
  GAGACAGATGTATACGACTCCTATAGGGCGGCCGCGAACTCTCAGATCTGGTACCGATAT
  CAAGCTTGTCGAGATTCGCTTAGCCGCGCTTTAAAAAGGTTTGCATCAGCTGTGAGTCCA
  TCTGACAAGCGAGGAAACTAAGGCTGAGAAGTGGGAGGCGTTGCCATCTGCAGGCCCAGG
  CAACCTGCTACGGGAAGACCGGGGACCAAGACCTCTGGGTTGGCTTTCCTAGACCCGCTC
  GGGTCTTCGGGTGTCGCGAGGAAGGGCCCTGCTCCTTTCGTTCCCTGCACCCCTGGCCGC
  TGCAGGTGGCTCCCTGGAGGAGGAGCTCCCACGCGGAGGAGGAGCCAGGGCAGCTGGGAG
  CGGGGACACCATCCTCCTGGATAAGAGGCAGAGGCCGGGAGGAACCCCGTCAGCCGGGCG
  GGCAGGAAGCTCTGGGAGTAGCCTCATGGAAGAGAAGCAGATCCTGTGCGTGGGGCTAGT
  GGTGCTGGACGTCATCAGCCTGGTGGACAAGTACCCTAAGGAGGACTCGGAGATAAGGTG
  TTTGTCCCAGAGATGGCAGCGCGGAGGCAACGCGTCCAACTCCTGCACCATTCTCTCCCT
  GCTCGGAGCCCCCTGTGCCTTCATGGGCTCAATGGCTCCTGGCCATGTTGCTGATTTTGT
  CCTGGATGACCTCCGCCGTTATTCTGTGGACCTACGCTACACAGTCTTTCAGACCACAGG
  CTCCGTCCCCATCGCCACGGTCATCATCAACGAGGCCAGTGGTAGCCGCACCATCCTATA
  CTATGACAGGAGCCTGCCAGATGTGTCTGCTACAGACTTTGAGATGTTGATCTGACCCAG
  TTCAAGTGGATCCACATTGAGGGCCGGACGCATCGGAGCAGTGAAGATGCTGCAGCGATA
  GACGCACACACACCAGCAGCCTCCAGAGCAGAGATCCGGTGTCCGTGGGAGGTGGAGAAG
  CCACGGAGAGGAGCTCTTTCAG
  
• Restriction Sites: Please inquire
• ACCN: NM_000221
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a good match to NM_000221.2 except for one SNP.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000221.1, NP_000212.1
• RefSeq Size: 1899 bp
• RefSeq ORF: 897 bp
• Locus ID: 3795
• Cytogenetics: 2p23.3
• Domains: pfkB
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant utilizes the alternate exon 3a and encodes isoform a, which exhibits lower enzymatic activity than isoform b.