SCN3A (NM_001081676) Human Untagged Clone

CAT#: SC316037

SCN3A (untagged)-Human sodium channel, voltage-gated, type III, alpha subunit (SCN3A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCN3A
• Synonyms: EIEE62; FFEVF4; NAC3; Nav1.3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001081676 edited
  AGAGAGAAAAAAAATTGTAACTAATTTGTAAACCTCTGTGGTCAAAAAAAAAAAAAAAAA
  AAAAAAGCTGAACAGCTGCAGAGGAAGACACGTTATACCCTAACCATCTTGGATGCTGGG
  CTTTGTTATGCTGTAATTCATAAGGCTCTGTTTTATCAGAGATTATGGAGCAAGAAAACT
  GAAGCCAAGCCACATCAAGGTTTGACAGGGATGAGATACCTGTCAAGGATTCATAGTAGA
  GTGGCTTACTGGGAAAGGAGCAAAGAATCTCTTCTAGGGATATTGTAAGAATAAATGAGA
  TAATTCACAGAAGGGACCTGGAGCTTTTCCGGAAAAAGGTGCTGTGACTATCTAAGGTAA
  TTCGTATGCAAGAAGCTACACGTAATTAAATGTGCAGGATGAAAAGATGGCACAGGCACT
  GTTGGTACCCCCAGGACCTGAAAGCTTCCGCCTTTTTACTAGAGAATCTCTTGCTGCTAT
  CGAAAAACGTGCTGCAGAAGAGAAAGCCAAGAAGCCCAAAAAGGAACAAGATAATGATGA
  TGAGAACAAACCAAAGCCAAATAGTGACTTGGAAGCTGGAAAGAACCTTCCATTTATTTA
  TGGAGACATTCCTCCAGAGATGGTGTCAGAGCCCCTGGAGGACCTGGATCCCTACTATAT
  CAATAAGAAAACTTTTATAGTAATGAATAAAGGAAAGGCAATTTTCCGATTCAGTGCCAC
  CTCTGCCTTGTATATTTTAACTCCACTAAACCCTGTTAGGAAAATTGCTATCAAGATTTT
  GGTACATTCTTTATTCAGCATGCTTATCATGTGCACTATTTTGACCAACTGTGTATTTAT
  GACCTTGAGCAACCCTCCTGACTGGACAAAGAATGTAGAGTACACATTCACTGGAATCTA
  TACCTTTGAGTCACTTATAAAAATCTTGGCAAGAGGGTTTTGCTTAGAAGATTTTACGTT
  TCTTCGTGATCCATGGAACTGGCTGGATTTCAGTGTCATTGTGATGGCATATGTGACAGA
  GTTTGTGGACCTGGGCAATGTCTCAGCGTTGAGAACATTCAGAGTTCTCCGAGCACTGAA
  AACAATTTCAGTCATTCCAGGTTTAAAGACCATTGTGGGGGCCCTGATCCAGTCGGTAAA
  GAAGCTTTCTGATGTGATGATCCTGACTGTGTTCTGTCTGAGCGTGTTTGCTCTCATTGG
  GCTGCAGCTGTTCATGGGCAATCTGAGGAATAAATGTTTGCAGTGGCCCCCAAGCGATTC
  TGCTTTTGAAACCAACACCACTTCCTACTTTAATGGCACAATGGATTCAAATGGGACATT
  TGTTAATGTAACAATGAGCACATTTAACTGGAAGGATTACATTGGAGATGACAGTCACTT
  TTATGTTTTGGATGGGCAAAAAGACCCTTTACTCTGTGGAAATGGCTCAGATGCAGGCCA
  GTGTCCAGAAGGATACATCTGTGTGAAGGCTGGTCGAAACCCCAACTATGGCTACACAAG
  CTTTGACACCTTTAGCTGGGCTTTCCTGTCTCTATTTCGACTCATGACTCAAGACTACTG
  GGAAAATCTTTACCAGTTGACATTACGTGCTGCTGGGAAAACATACATGATATTTTTTGT
  CCTGGTCATTTTCTTGGGCTCATTTTATTTGGTGAATTTGATCCTGGCTGTGGTGGCCAT
  GGCCTATGAGGAGCAGAATCAGGCCACCTTGGAAGAAGCAGAACAAAAAGAGGCCGAATT
  TCAGCAGATGCTCGAACAGCTTAAAAAGCAACAGGAAGAAGCTCAGGCAGTTGCGGCAGC
  ATCAGCTGCTTCAAGAGATTTCAGTGGAATAGGTGGGTTAGGAGAGCTGTTGGAAAGTTC
  TTCAGAAGCATCAAAGTTGAGTTCCAAAAGTGCTAAAGAATGGAGGAACCGAAGGAAGAA
  AAGAAGACAGAGAGAGCACCTTGAAGGAAACAACAAAGGAGAGAGAGACAGCTTTCCCAA
  ATCCGAATCTGAAGACAGCGTCAAAAGAAGCAGCTTCCTTTTCTCCATGGATGGAAACAG
  ACTGACCAGTGACAAAAAATTCTGCTCCCCTCATCAGTCTCTCTTGAGTATCCGTGGCTC
  CCTGTTTTCCCCAAGACGCAATAGCAAAACAAGCATTTTCAGTTTCAGAGGTCGGGCAAA
  GGATGTTGGATCTGAAAATGACTTTGCTGATGATGAACACAGCACATTTGAAGACAGCGA
  AAGCAGGAGAGACTCACTGTTTGTGCCGCACAGACATGGAGAGCGACGCAACAGTAACGG
  CACCACCACAGAAACGGAAGTCAGAAAGAGAAGGTTAAGCTCTTACCAGATTTCAATGGA
  GATGCTGGAGGATTCCTCTGGAAGGCAAAGAGCCGTGAGCATAGCCAGCATTCTGACCAA
  CACAATGGAAGAACTTGAAGAATCTAGACAGAAATGTCCGCCATGCTGGTATAGATTTGC
  CAATGTGTTCTTGATCTGGGACTGCTGTGATGCATGGTTAAAAGTAAAACATCTTGTGAA
  TTTAATTGTTATGGATCCATTTGTTGATCTTGCCATCACTATTTGCATTGTCTTAAATAC
  CCTCTTTATGGCCATGGAGCACTACCCCATGACTGAGCAATTCAGTAGTGTGTTGACTGT
  AGGAAACCTGGTCTTTACTGGGATTTTCACAGCAGAAATGGTTCTCAAGATCATTGCCAT
  GGATCCTTATTACTATTTCCAAGAAGGCTGGAATATCTTTGATGGAATTATTGTCAGCCT
  CAGTTTAATGGAGCTTGGTCTGTCAAATGTGGAGGGATTGTCTGTACTGCGATCATTCAG
  ACTGCTTAGAGTTTTCAAGTTGGCAAAATCCTGGCCCACACTAAATATGCTAATTAAGAT
  CATTGGCAATTCTGTGGGGGCTCTAGGAAACCTCACCTTGGTGTTGGCCATCATCGTCTT
  CATTTTTGCTGTGGTCGGCATGCAGCTCTTTGGTAAGAGCTACAAAGAATGTGTCTGCAA
  GATCAATGATGACTGTACGCTCCCACGGTGGCACATGAACGACTTCTTCCACTCCTTCCT
  GATTGTGTTCCGCGTGCTGTGTGGAGAGTGGATAGAGACCATGTGGGACTGTATGGAGGT
  CGCTGGCCAAACCATGTGCCTTATTGTTTTCATGTTGGTCATGGTCATTGGAAACCTTGT
  GGTTCTGAACCTCTTTCTGGCCTTATTGTTGAGTTCATTTAGCTCAGACAACCTTGCTGC
  TACTGATGATGACAATGAAATGAATAATCTGCAGATTGCAGTAGGAAGAATGCAAAAGGG
  AATTGATTATGTGAAAAATAAGATGCGGGAGTGTTTCCAAAAAGCCTTTTTTAGAAAGCC
  AAAAGTTATAGAAATCCATGAAGGCAATAAGATAGACAGCTGCATGTCCAATAATACTGG
  AATTGAAATAAGCAAAGAGCTTAATTATCTTAGAGATGGGAATGGAACCACCAGTGGTGT
  AGGTACTGGAAGCAGTGTTGAAAAATACGTAATCGATGAAAATGATTATATGTCATTCAT
  AAACAACCCCAGCCTCACCGTCACAGTGCCAATTGCTGTTGGAGAGTCTGACTTTGAAAA
  CTTAAATACTGAAGAGTTCAGCAGTGAGTCAGAACTAGAAGAAAGCAAAGAGAAATTAAA
  TGCAACCAGCTCATCTGAAGGAAGCACAGTTGATGTTGTTCTACCCCGAGAAGGTGAACA
  AGCTGAAACTGAACCCGAAGAAGACCTTAAACCGGAAGCTTGTTTTACTGAAGGATGTAT
  TAAAAAGTTTCCATTCTGTCAAGTAAGTACAGAAGAAGGCAAAGGGAAGATCTGGTGGAA
  TCTTCGAAAAACCTGCTACAGTATTGTTGAGCACAACTGGTTTGAGACTTTCATTGTGTT
  CATGATCCTTCTCAGTAGTGGTGCATTGGCCTTTGAAGATATATACATTGAACAGCGAAA
  GACTATCAAAACCATGCTAGAATATGCTGACAAAGTCTTTACCTATATATTCATTCTGGA
  AATGCTTCTCAAATGGGTTGCTTATGGATTTCAAACATATTTCACTAATGCCTGGTGCTG
  GCTAGATTTCTTGATCGTTGATGTTTCTTTGGTTAGCCTGGTAGCCAATGCTCTTGGCTA
  CTCAGAACTCGGTGCCATCAAATCATTACGGACATTAAGAGCTTTAAGACCTCTAAGAGC
  CTTATCCCGGTTTGAAGGCATGAGGGTGGTTGTGAATGCTCTTGTTGGAGCAATTCCCTC
  TATCATGAATGTGCTGTTGGTCTGTCTCATCTTCTGGTTGATCTTTAGCATCATGGGTGT
  GAATTTGTTTGCTGGCAAGTTCTACCACTGTGTTAACATGACAACGGGTAACATGTTTGA
  CATTAGTGATGTTAACAATTTGAGTGACTGTCAGGCTCTTGGCAAGCAAGCTCGGTGGAA
  AAACGTGAAAGTAAACTTTGATAATGTTGGCGCTGGCTATCTTGCACTGCTTCAAGTGGC
  CACATTTAAAGGCTGGATGGATATTATGTATGCAGCTGTTGATTCACGAGATGTTAAACT
  TCAGCCTGTATATGAAGAAAATCTGTACATGTATTTATACTTTGTCATCTTTATCATCTT
  TGGGTCATTCTTCACTCTGAATCTATTCATTGGTGTCATCATAGATAACTTCAACCAGCA
  GAAAAAGAAGTTTGGAGGTCAAGACATCTTTATGACAGAGGAACAGAAAAAATATTACAA
  TGCAATGAAGAAACTTGGATCCAAGAAACCTCAGAAACCCATACCTCGCCCAGCAAACAA
  ATTCCAAGGAATGGTCTTTGATTTTGTAACCAGACAAGTCTTTGATATCAGCATCATGAT
  CCTCATCTGCCTCAACATGGTCACCATGATGGTGGAAACGGATGACCAGGGCAAATACAT
  GACCCTAGTTTTGTCCCGGATCAACCTAGTGTTCATTGTTCTGTTCACTGGAGAATTTGT
  GCTGAAGCTCGTCTCCCTCAGACACTACTACTTCACTATAGGCTGGAACATCTTTGACTT
  TGTGGTGGTGATTCTCTCCATTGTAGGTATGTTTCTGGCTGAGATGATAGAAAAGTATTT
  TGTGTCCCCTACCTTGTTCCGAGTGATCCGTCTTGCCAGGATTGGCCGAATCCTACGTCT
  GATCAAAGGAGCAAAGGGGATCCGCACGCTGCTCTTTGCTTTGATGATGTCCCTTCCTGC
  GTTGTTTAACATCGGCCTCCTGCTCTTCCTGGTCATGTTTATCTATGCCATCTTTGGGAT
  GTCCAACTTTGCCTATGTTAAAAAGGAAGCTGGAATTGATGACATGTTCAACTTTGAGAC
  CTTTGGCAACAGCATGATCTGCTTGTTCCAAATTACAACCTCTGCTGGCTGGGATGGATT
  GCTAGCACCTATTCTTAATAGTGCACCACCCGACTGTGACCCTGACACAATTCACCCTGG
  CAGCTCAGTTAAGGGAGACTGTGGGAACCCATCTGTTGGGATTTTCTTTTTTGTCAGTTA
  CATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATCGCGGTCATCCTGGAGAACTT
  CAGTGTTGCTACTGAAGAAAGTGCAGAGCCCCTGAGTGAGGATGACTTTGAGATGTTCTA
  TGAGGTTTGGGAAAAGTTTGATCCCGATGCGACCCAGTTTATAGAGTTCTCTAAACTCTC
  TGATTTTGCAGCTGCCCTGGATCCTCCTCTTCTCATAGCAAAACCCAACAAAGTCCAGCT
  TATTGCCATGGATCTGCCCATGGTCAGTGGTGACCGGATCCACTGTCTTGATATTTTATT
  TGCCTTTACAAAGCGTGTTTTGGGTGAGAGTGGAGAGATGGATGCCCTTCGAATACAGAT
  GGAAGACAGGTTTATGGCATCAAACCCCTCCAAAGTCTCTTATGAGCCTATTACAACCAC
  TTTGAAACGTAAACAAGAGGAGGTGTCTGCCGCTATCATTCAGCGTAATTTCAGATGTTA
  TCTTTTAAAGCAAAGGTTAAAAAATATATCAAGTAACTATAACAAAGAGGCAATTAAAGG
  GAGGATTGACTTACCTATAAAACAAGACATGATTATTGACAAACTAAATGGGAACTCCAC
  TCCAGAAAAAACAGATGGGAGTTCCTCTACCACCTCTCCTCCTTCCTATGATAGTGTAAC
  AAAACCAGACAAGGAAAAGTTTGAGAAAGACAAACCAGAAAAAGAAAGCAAAGGAAAAGA
  GGTCAGAGAAAATCAAAAGTAAAAAGAAACAAAGAATTATCTTTGTGATCAATTGTTTAC
  AGCCTATGAAGGTAAAGTATATGTGTCAACTGGACTTCAAGAGGAGGTCCATGCCAAACT
  GACTGTTTTAACAAATACTCATAGTCAGTGCCTATACAAGACAGTGAAGTGACCTCTCTG
  TCACTGCAACTCTGTGAAGCAGGGTATCAACATTGACAAGAGGTTGCTGTTTTTATTACC
  AGCTGACACTGCTGAGGAGAAACCCAATGGCTACCTAGACTATAGGGATAGTTGTGCAAA
  GTGAACATTGTAACTACACCAAACACCTTTAGTACAGTCCTTGCATCCATTCTATTTTTA
  ACTTCCATATCTGCCATATTTTTACAAAATTTGTTCTAGTGCATTTCCATGGTCCCCAAT
  TCATAGTTTATTCATAATGCTATGTCACTATTTTTGTAAATGAGGTTTACGTTGAAGAAA
  CAGTATACAAGAACCCTGTCTCTCAAATGATCAGACAAAGGTGTTTTGCCAGAGAGATAA
  AATTTTTGCTCAAAACCAGAAAAAGAATTGTAATGGCTACAGTTTCAGTTACTTCCATTT
  TCTAGATGGCTTTAATTTTGAAAGTATTTTAGTCTGTTATGTTTGTTTCTATCTGAACAG
  TTATGTGCCTGTAAAGTCTCCTCTAATATTTAAAGGATTATTTTTATGCAAAGTATTCTG
  TTTCAGCAAGTGCAAATTTTATTCTAAGTTTCAGAGCTCTATATTTAATTTAGGTCAAAT
  GCTTTCCAAAAAGTAATCTAATAAATCCATTCTAGAAAAATATATCTAAAGTATTGCTTT
  AGAATAGTTGTTCCACTTTCTGCTGCAGTATTGCTTTGCCATCTTCTGCTCTCAGCAAAG
  CTGATAGTCTATGTCAATTAAATACCCTATGTTATGTAAATAGTTATTTTATCCTGTGGT
  GCATGTTTGGGCAAATATATATATAGCCTGATAAACAACTTCTATTAAATCAAATATGTA
  CCACAGTGTATGTGTCTTTTGCAAGCTTCCAACAGGGATGTATCCTGTATCATTCATTAA
  ACATAGTTTAAAGGCTATCACTAATGCATGTTAATATTGCCTATGCTGCTCTATTTTACT
  CAATCCATTCTTCACAAGTCTTGGTTAAAGAATGTCACATATTGGTGATAGAATGAATTC
  AACCTGCTCTGTCCATTATGTCAAGCAGAATAATTTGAAGCTATTTACAAACACCTTTAC
  TTTTGCACTTTTAATTCAACATGAGTATCATATGGTATCTCTCTAGATTTCAAGGAAACA
  CACTGGATACTGCCTACTGACAAAACCTATTCTTCATATTTTGCTAAAAATATGTCTAAA
  ACTTGTTTAAATATAAATAATGTAAAAATATAATCAACTTTATTTGTCAGCATTTTGTAC
  ATAAGAAAATTATTTTCAGGTTGATGACATCACAATTTATTTTACTTTATGCTTTTGCTT
  TTGATTTTTAATCACAATTCCAAACTTTTGAATCCATAAGATTTTTCAATGGATAATTTC
  CTAAAATAAAAGTTAGATAATGGGTTTTATGGATTTCTTTGTTATAATATATTTTCTACC
  ATTCCAATAGGAGATACATTGGTCAAACACTCAAACCTAGATCATTTTCTACCAACTATG
  GTTGCCTCAATATAACCTTTTATTCATAGATGTTTTTTTTTATTCAACTTTTGTAGTATT
  TACGTATGCAGACTAGTCTTATTTTTTTAATTCCTGCTGCACTAAAGCTATTACAAATAT
  AACATGGACTTTGTTCTTTTTAGCCATGAACAAAGTGGCAAAGTTGTGCAATTACCTAAC
  ATGATATAAATTTTTGTTTTTTGCACAAACCAAAAGTTTAATGTTAATTCTTTTTACAAA
  ACTATTTACTGTAGTGTATTGAAGAACTGCATGCAGGGAATTGCTATTGCTAAAAAGAAT
  GGTGAGCTACGTCATTATTGAGCCAAAAGAATAAATTTCATTTTTTATTGCATTTCACTT
  ATTGGCCTCTGGGGTTTTTTGTTTTTGTTTTTTGCTGTTGGCAGTTTAAAATATATATAA
  TTAATAAAACCTGTGCTTGATCTGACATTTGTATACATAAAAGTTTACATGAATTTTACA
  ACAAACTAGTGCATGATTCACCAAGCAGTACTACAGAACAAAGGCAAATTAAAAGCAGCT
  TTGTGAACTTTTATGTGTGCAAAGGATCAAGTTCACATGTTCCAACTTTCAGGTTTGATA
  ATAATAGTAGTAACCACCTACAATAGCTTTCAATTTCAATTAACTCCCTTGGCTATAAGC
  ATCTAAACTCATCTTCTTTCAATATAATTGATGCTATCTCCTAATTACTTGGTGGCTAAT
  AAATGTTACATTCTTTGTTACTTAAATGCATTATATAAACTCCTATGTATACATAAGGTA
  TTAATGATATAGTTATTGAGAATTTATATTAACTTTTTTTTCAAGAACCCTTGGATTTAT
  GTGAGGTCAAAACCAAACTCTTATTCTCAGTGGAAAACTCCAGTTGTAATGCATATTTTT
  AAAGACAATTTGGATCTAAATATGTATTTCATAATTCTCCCATAATAAATTATATAAGGT
  GGCCAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001081676
• Insert Size: 8900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081676.1, NP_001075145.1
• RefSeq Size: 8994 bp
• RefSeq ORF: 5856 bp
• Locus ID: 6328
• Cytogenetics: 2q24.3
• Protein Families: Druggable Genome, Ion Channels: Sodium, Transmembrane
• Gene Summary: 'Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family, and is found in a cluster of five alpha subunit genes on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2), also known as 12v1, SCN3A-s, or the adult form, uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a shorter protein (isoform 2).