ERG (NM_004449) Human Untagged Clone

CAT#: SC316039

ERG (untagged)-Human v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERG
• Synonyms: erg-3; p55
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004449 edited
  ATGATTCAGACTGTCCCGGACCCAGCAGCTCATATCAAGGAAGCCTTATCAGTTGTGAGT
  GAGGACCAGTCGTTGTTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGACAGAGATG
  ACCGCGTCCTCCTCCAGCGACTATGGACAGACTTCCAAGATGAGCCCACGCGTCCCTCAG
  CAGGATTGGCTGTCTCAACCCCCAGCCAGGGTCACCATCAAAATGGAATGTAACCCTAGC
  CAGGTGAATGGCTCAAGGAACTCTCCTGATGAATGCAGTGTGGCCAAAGGCGGGAAGATG
  GTGGGCAGCCCAGACACCGTTGGGATGAACTACGGCAGCTACATGGAGGAGAAGCACATG
  CCACCCCCAAACATGACCACGAACGAGCGCAGAGTTATCGTGCCAGCAGATCCTACGCTA
  TGGAGTACAGACCATGTGCGGCAGTGGCTGGAGTGGGCGGTGAAAGAATATGGCCTTCCA
  GACGTCAACATCTTGTTATTCCAGAACATCGATGGGAAGGAACTGTGCAAGATGACCAAG
  GACGACTTCCAGAGGCTCACCCCCAGCTACAACGCCGACATCCTTCTCTCACATCTCCAC
  TACCTCAGAGAGACTCCTCTTCCACATTTGACTTCAGATGATGTTGATAAAGCCTTACAA
  AACTCTCCACGGTTAATGCATGCTAGAAACACAGATTTACCATATGAGCCCCCCAGGAGA
  TCAGCCTGGACCGGTCACGGCCACCCCACGCCCCAGTCGAAAGCTGCTCAACCATCTCCT
  TCCACAGTGCCCAAAACTGAAGACCAGCGTCCTCAGTTAGATCCTTATCAGATTCTTGGA
  CCAACAAGTAGCCGCCTTGCAAATCCAGGCAGTGGCCAGATCCAGCTTTGGCAGTTCCTC
  CTGGAGCTCCTGTCGGACAGCTCCAACTCCAGCTGCATCACCTGGGAAGGCACCAACGGG
  GAGTTCAAGATGACGGATCCCGACGAGGTGGCCCGGCGCTGGGGAGAGCGGAAGAGCAAA
  CCCAACATGAACTACGATAAGCTCAGCCGCGCCCTCCGTTACTACTATGACAAGAACATC
  ATGACCAAGGTCCATGGGAAGCGCTACGCCTACAAGTTCGACTTCCACGGGATCGCCCAG
  GCCCTCCAGCCCCACCCCCCGGAGTCATCTCTGTACAAGTACCCCTCAGACCTCCCGTAC
  ATGGGCTCCTATCACGCCCACCCACAGAAGATGAACTTTGTGGCGCCCCACCCTCCAGCC
  CTCCCCGTGACATCTTCCAGTTTTTTTGCTGCCCCAAACCCATACTGGAATTCACCAACT
  GGGGGTATATACCCCAACACTAGGCTCCCCACCAGCCATATGCCTTCTCATCTGGGCACT
  TACTACTAAAGACCTGGCGGAGGCTTTTCCCATCAGCGTGCATTCACCAGCCCATCGCCA
  CAAACTCTATCGGAGAACATGAATCAAAAGTGCCTCAAGAGGAATGAAAAAAGCTTTACT
  GGGGCTGGGGAAGGAAGCCGGGGAAGAGATCCAAAGACTCTTGGGAGGGAGTTACTGAAG
  TCTTACTACAGAAATGAGGAGGATGCTAAAAATGTCACGAATATGGACATATCATCTGTG
  GACTGACCTTGTAAAAGACAGTGTATGTAGAAGCATGAAGTCTTAAGGACAAAGTGCCAA
  AGAAAGTGGTCTTAAGAAATGTATAAACTTTAGAGTAGAGTTTGGAATCCCACTAATGCA
  AACTGGGATGAAACTAAAGCAATAGAAACAACACAGTTTTGACCTAACATACCGTTTATA
  ATGCCATTTTAAGGAAAACTACCTGTATTTAAAAATAGAAACATATCAAAAACAAGAGAA
  AAGACACGAGAGAGACTGTGGCCCATCAACAGACGTTGATATGCAACTGCATGGCATGTG
  CTGTTTTGGTTGAAATCAAATACATTCCGTTTGATGGACAGCTGTCAGCTTTCTCAAACT
  GTGAAGATGACCCAAAGTTTCCAACTCCTTTACAGTATTACCGGGACTATGAACTAAAAG
  GTGGGACTGAGGATGTGTATAGAGTGAGCGTGTGATTGTAGACAGAGGGGTGAAGAAGGA
  GGAGGAAGAGGCAGAGAAGGAGGAGACCAGGCTGGGAAAGAAACTTCTCAAGCAATGAAG
  ACTGGACTCAGGACATTTGGGGACTGTGTACAATGAGTTATGGAGACTCGAGGGTTCATG
  CAGTCAGTGTTATACCAAACCCAGTGTTAGGAGAAAGGACACAGCGTAATGGAGAAAGGG
  AAGTAGTAGAATTCAGAAACAAAAATGCGCATCTCTTTCTTTGTTTGTCAAATGAAAATT
  TTAACTGGAATTGTCTGATATTTAAGAGAAACATTCAGGACCTCATCATTATGTGGGGGC
  TTTGTTCTCCACAGGGTCAGGTAAGAGATGGCCTTCTTGGCTGCCACAATCAGAAATCAC
  GCAGGCATTTTGGGTAGGCGGCCTCCAGTTTTCCTTTGAGTCGCGAACGCTGTGCGTTTG
  TCAGAATGAAGTATACAAGTCAATGTTTTTCCCCCTTTTTATATAATAATTATATAACTT
  ATGCATTTATACACTACGAGTTGATCTCGGCCAGCCAAAGACACACGACAAAAGAGACAA
  TCGATATAATGTGGCCTTGAATTTTAACTCTGTATGCTTAATGTTTACAATATGAAGTTA
  TTAGTTCTTAGAATGCAGAATGTATGTAATAAAATAAGCTTGGCCTAGCATGGCAAATCA
  GATTTAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004449 unedited
  GGACACCTGTGTATACGACTCCTATAGGGCGGCTGCGTATCTTCGGCACGAGGTACTGAT
  TCAGACTGTCCCGGACCCAGCAGCTCATATCAAGGAAGCCTTATCAGTTGTGAGTGAGGA
  CCAGTCGTTGTTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGACAGAGATGACCGC
  GTCCTCCTCCAGCGACTATGGACAGACTTCCAAGATGAGCCCACGCGTCCCTCAGCAGGA
  TTGGCTGTCTCAACCCCCAGCCAGGGTCACCATCAAAATGGAATGTAACCCTAGCCAGGT
  GAATGGCTCAAGGAACTCTCCTGATGAATGCAGTGTGGCCAAAGGCGGGAAGATGGTGGG
  CAGCCCAGACACCGTTGGGATGAACTACGGCAGCTACATGGAGGAGAAGCACATGCCACC
  CCCAAACATGACCACGAACGAGCGCAGAGTTATCGTGCCAGCAGATCCTACGCTATGGAG
  TACAGACCATGTGCGGCAGTGGCTGGAGTGGGCGGTGAAAGAATATGGCCTTCCAGACGT
  CAACATCTTGTTATTCCAGAACATCGATGGGAAGGAACTGTGCAAGATGACCAAGGACGA
  CTTCCAGAGGCTCACCCCCAGCTACAACGCCGACATCCTTCTCTCACATCTCCACTACCT
  CAGAGAGACTCCTCTTCCACATTTGACTTCAGATGATGTTGATAAAGCCTTACAAAACTC
  TCCACGGTTAATGCATGCTAGAAACACAGATTTACCATATGAGCCCCCCAGGAGATCAGC
  CTGGACCGGTCACGGCACCCCCACGCCCCAGTCGAAAGCTGCTCAACCATCTCTCACAGT
  GCCCAAAAACTGAAGACCAGCGTCCTCAGTAGATCCTTATCAGATTCTTGGACCAACCAA
  GTAGGCCGCCTTGCAAATCCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_004449 unedited
  CGGAGAAGTCACTTCGGGCCGGATGGCACTTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTAAATCTGATTTGCCATGCTAGGCCGAGCTTATTTTATTACATACATTCTGCATTC
  TAAGAACTAATAACTTCATATTGTAAACATTAAGCATACAGAGTTAAAATTCAAGGCCAC
  ATTATATCGATTGTCTCTTTTGTCGTGTGTCTTTGGCTGGCCGAGATCAACTCGTAGTGT
  ATAAATGCATAAGTTATATAATTATTATATAAAAAGGGGGAAAAACATTGACTTGTATAC
  TTCATTCTGACAAACGCACAGCGTTCGCGACTCAAAGGAAAACTGGAGGCCGCCTACCCA
  AAATGCCTGCGTGATTTCTGATTGTGGCAGCCAAGAAGGCCATCTCTTACCTGACCCTGT
  GGAGAACAAAGCCCCCACATAATGATGAGGTCCTGAATGTTTCTCTTAAATATCACACAA
  TTCCAGTTAAAATTTTCATTTGACAAACAAAGAAAGAGATGCGCATTTTTGTTTCTGAAT
  TCTACTACTTCCCCTTTCTCCATTACGCTGTGTCCTTTCTCCTAACACTGGGTTTGGTAT
  AACACTGACTGCATGAACCCTCGAGTCTCCATAACTCATTGTACACAGTCCCCAAATGTC
  CTGAGTCCAGTCTTCATTGCTTGAGAAGTTTCTTTAGCAGACCCTGGTCTGCTCCTTCTC
  TGCCTGTTGCCTCCTCCTTCTTCACCCCTCTGTCTACAATCACACGCTCACTCTATACAC
  ATCTCAGATCCCACCTTTTAGTTCATAGTGCCCGTCATACTGTAAAGCAGTTCGAAACTT
  TCGGGTCATCTTTCACCAGTTTGAGCAAGC
  
• Restriction Sites: Please inquire
• ACCN: NM_004449
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004449.3, NP_004440.1
• RefSeq Size: 3097 bp
• RefSeq ORF: 1389 bp
• Locus ID: 2078
• Cytogenetics: 21q22.2
• Domains: ETS, SAM_PNT
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 3. The resulting protein (isoform 2) is shorter when it is compared to isoform 3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.