SEPP1 (SELENOP) (NM_001093726) Human Untagged Clone

CAT#: SC316200

SEPP1 (untagged)-Human selenoprotein P, plasma, 1 (SEPP1), transcript variant 3 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: SELENOP
• Synonyms: SELP; SeP; SEPP; SEPP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001093726 edited
  CAAAGAGAAAGGAAGCAGGCCCGTTGGAAGTGGTTGTCTACTTGCAATATGTGCTCTGGG
  CTGGACGCGGTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCGGATC
  ACGAGGACAACCCCAGCAATGTGGAGAAGCCTGGGGCTTGCCCTGGCTCTCTGTCTCCTC
  CCATCGGGAGGAACAGAGAGCCAGGACCAAAGCTCCTTATGTAAGCAACCCCCAGCCTGG
  AGCATAAGAGATCAAGATCCAATGCTAAACTCCAATGGTTCAGTGACTGTGGTTGCTCTT
  CTTCAAGCCAGCTGATACCTGTGCATACTGCAGGCATCTAAATTAGAAGACCTGCGAGTA
  AAACTGAAGAAAGAAGGATATTCTAATATTTCTTATATTGTTGTTAATCATCAAGGAATC
  TCTTCTCGATTAAAATACACACATCTTAAGAATAAGGTTTCAGAGCATATTCCTGTTTAT
  CAACAAGAAGAAAACCAAACAGATGTCTGGACTCTTTTAAATGGAAGCAAAGATGACTTC
  CTCATATATGATAGATGTGGCCGTCTTGTATATCATCTTGGTTTGCCTTTTTCCTTCCTA
  ACTTTCCCATATGTAGAAGAAGCCATTAAGATTGCTTACTGTGAAAAGAAATGTGGAAAC
  TGCTCTCTCACGACTCTCAAAGATGAAGACTTTTGTAAACGTGTATCTTTGGCTACTGTG
  GATAAAACAGTTGAAACTCCATCGCCTCATTACCATCATGAGCATCATCACAATCATGGA
  CATCAGCACCTTGGCAGCAGTGAGCTTTCAGAGAATCAGCAACCAGGAGCACCAAATGCT
  CCTACTCATCCTGCTCCTCCAGGCCTTCATCACCACCATAAGCACAAGGGTCAGCATAGG
  CAGGGTCACCCAGAGAACCGAGATATGCCAGCAAGTGAAGATTTACAAGATTTACAAAAG
  AAGCTCTGTCGAAAGAGATGTATAAATCAATTACTCTGTAAATTGCCCACAGATTCAGAG
  TTGGCTCCTAGGAGCTGATGCTGCCATTGTCGACATCTGATATTTGAAAAAACAGGGTCT
  GCAATCACCTGACAGTGTAAAGAAAACCTCCCATCTTTATGTAGCTGACAGGGACTTCGG
  GCAGAGGAGAACATAACTGAATCTTGTCAGTGACGTTTGCCTCCAGCTGCCTGACAAATA
  AGTCAGCAGCTTATACCCACAGAAGCCAGTGCCAGTTGACGCTGAAAGAATCAGGCAAAA
  AAGTGAGAATGACCTTCAAACTAAATATTTAAAATAGAACATACTCCCCAATTTAGTCTA
  GACACAATTTCATTTCCAGCATTTTTATAAACTACCAAATTAGTGAACCAAAAATAGAAA
  TTAGATTTGTGCAAACATGGAGAAATCTACTGAATTGGCTTCCAGATTTTAAATTTTATG
  TCATAGAAATATTGACTCAAACCATATTTTTTATGATGGAGCAACTGAAAGGTGATTGCA
  GCTTTTGGTTAATATGTCTTTTTTTTTCTTTTTCCAGTGTTCTATTTGCTTTAATGAGAA
  TAGAAACGTAAACTATGACCTAGGGGTTTCTGTTGGATAATTAGCAGTTTAGAATGGAGG
  AAGAACAACAAAGACATGCTTTCCATTTTTTTCTTTACTTATCTCTCAAAACAATATTAC
  TTTGTCTTTTCAATCTTCTACTTTTAACTAATAAAATAAGTGGATTTTGTATTTTAAGAT
  CCAGAAATACTTAACACGTGAATATTTTGCTAAAAAAGCATATATAACTATTTTAAATAT
  CCATTTATCTTTTGTATATCTAAGACTCATCCTGATTTTTACTATCACACATGAATAAAG
  CCTTTGTATCTTTCTTTCTCTAATGTTGTATCATACTCTTCTAAAACTTGAGTGGCTGTC
  TTAAAAGATATAAGGGGAAAGATAATATTGTCTGTCTCTATATTGCTTAGTAAGTATTTC
  CATAGTCAATGATGGTTTAATAGGTAAACCAAACCCTATAAACCTGACCTCCTTTATGGT
  TAATACTATTTAAGCAAGAATGCAGTACAGAATTGGATACAGTACGGATTTGTCCAAATA
  AATTCAATAAAAACCTTAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001093726
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001093726.1, NP_001087195.1
• RefSeq Size: 2252 bp
• Locus ID: 6414
• Cytogenetics: 5p12
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in human), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. The use of alternative polyadenylation sites, one located in between the two SECIS elements, results in two populations of mRNAs containing either both (predominant) or just the upstream SECIS element (PMID:27881738). Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Oct 2018]'
  Transcript Variant: This variant (3, also known as Sepp1b) contains an additional 5' coding exon compared to variant 1. It initiates translation from an in-frame, upstream start codon, which results in an isoform (2) with a longer and distinct N-terminus compared to isoform 1.