GCNT1 (NM_001097633) Human Untagged Clone

CAT#: SC316213

GCNT1 (untagged)-Human glucosaminyl (N-acetyl) transferase 1, core 2 (GCNT1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCNT1
• Synonyms: C2GNT; C2GNT-L; C2GNT1; G6NT; NACGT2; NAGCT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC316213 ORF sequence for NM_001097633, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGAGGACGTTGCTGCGAAGGAGACTTTTTTCTTATCCCACCAAATACTACTTTATGGTTCTTGTTT
  TATCCCTAATCACCTTCTCCGTTTTAAGGATTCATCAAAAGCCTGAATTTGTAAGTGTCAGACACTTGGA
  GCTTGCTGGGGAGAATCCTAGTAGTGATATTAATTGCACCAAAGTTTTACAGGGTGATGTAAATGAAATC
  CAAAAGGTAAAGCTTGAGATCCTAACAGTGAAATTTAAAAAGCGCCCTCGGTGGACACCTGACGACTATA
  TAAACATGACCAGTGACTGTTCTTCTTTCATCAAGAGACGCAAATATATTGTAGAACCCCTTAGTAAAGA
  AGAGGCGGAGTTTCCAATAGCATATTCTATAGTGGTTCATCACAAGATTGAAATGCTTGACAGGCTGCTG
  AGGGCCATCTATATGCCTCAGAATTTCTATTGCATTCATGTGGACACAAAATCCGAGGATTCCTATTTAG
  CTGCAGTGATGGGCATCGCTTCCTGTTTTAGTAATGTCTTTGTGGCCAGCCGATTGGAGAGTGTGGTTTA
  TGCATCGTGGAGCCGGGTTCAGGCTGACCTCAACTGCATGAAGGATCTCTATGCAATGAGTGCAAACTGG
  AAGTACTTGATAAATCTTTGTGGTATGGATTTTCCCATTAAAACCAACCTAGAAATTGTCAGGAAGCTCA
  AGTTGTTAATGGGAGAAAACAACCTGGAAACGGAGAGGATGCCATCCCATAAAGAAGAAAGGTGGAAGAA
  GCGGTATGAGGTCGTTAATGGAAAGCTGACAAACACAGGGACTGTCAAAATGCTTCCTCCACTCGAAACA
  CCTCTCTTTTCTGGCAGTGCCTACTTCGTGGTCAGTAGGGAGTATGTGGGGTATGTACTACAGAATGAAA
  AAATCCAAAAGTTGATGGAGTGGGCACAAGACACATACAGCCCTGATGAGTATCTCTGGGCCACCATCCA
  AAGGATTCCTGAAGTCCCGGGCTCACTCCCTGCCAGCCATAAGTATGATCTGTCTGACATGCAAGCAGTT
  GCCAGGTTTGTCAAGTGGCAGTACTTTGAGGGTGATGTTTCCAAGGGTGCTCCCTACCCGCCCTGCGATG
  GAGTCCATGTGCGCTCAGTGTGCATTTTCGGAGCTGGTGACTTGAACTGGATGCTGCGCAAACACCACTT
  GTTTGCCAATAAGTTTGACGTGGATGTTGACCTCTTTGCCATCCAGTGTTTGGATGAGCATTTGAGACAC
  AAAGCTTTGGAGACATTAAAACACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001097633
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001097633.1, NP_001091102.1
• RefSeq Size: 5488 bp
• RefSeq ORF: 1287 bp
• Locus ID: 2650
• Cytogenetics: 9q21.13
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: 'This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1, and uses the P2 promoter.