MGAT3 (NM_001098270) Human Untagged Clone

CAT#: SC316241

MGAT3 (untagged)-Human mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase (MGAT3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT3
• Synonyms: GNT-III; GNT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001098270, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGATGAGACGCTACAAGCTCTTTCTCATGTTCTGTATGGCCGGCCTGTGCCTCATCTCCTTCCTGC
  ACTTCTTCAAGACCCTGTCCTATGTCACCTTCCCCCGAGAACTGGCCTCCCTCAGCCCTAACCTGGTGTC
  CAGCTTTTTCTGGAACAATGCCCCGGTCACGCCCCAGGCCAGCCCCGAGCCAGGAGGCCCTGACCTGCTG
  CGTACCCCACTCTACTCCCACTCGCCCCTGCTGCAGCCGCTGCCGCCCAGCAAGGCGGCCGAGGAGCTCC
  ACCGGGTGGACTTGGTGCTGCCCGAGGACACCACCGAGTATTTCGTGCGCACCAAGGCCGGCGGCGTCTG
  CTTCAAACCCGGCACCAAGATGCTGGAGAGGCCGCCCCCGGGACGGCCGGAGGAGAAGCCTGAGGGGGCC
  AACGGCTCCTCGGCCCGGCGGCCACCCCGGTACCTCCTGAGCGCCCGGGAGCGCACGGGGGGCCGAGGCG
  CCCGGCGCAAGTGGGTGGAGTGCGTGTGCCTGCCCGGCTGGCACGGACCCAGCTGCGGCGTGCCCACTGT
  GGTGCAGTACTCCAACCTGCCCACCAAGGAGCGGCTGGTGCCCAGGGAGGTGCCGCGCCGCGTCATCAAC
  GCCATCAACGTCAACCACGAGTTCGACCTGCTGGACGTGCGCTTCCACGAGCTGGGCGACGTGGTGGACG
  CCTTTGTGGTGTGCGAGTCCAACTTCACGGCTTATGGGGAGCCGCGGCCGCTCAAGTTCCGGGAGATGCT
  GACCAATGGCACCTTCGAGTACATCCGCCACAAGGTGCTCTATGTCTTCCTGGACCACTTCCCGCCCGGC
  GGCCGGCAGGACGGCTGGATCGCCGACGACTACCTGCGCACCTTCCTCACCCAGGACGGCGTCTCGCGGC
  TGCGCAACCTGCGGCCCGACGACGTCTTCATCATTGACGATGCGGACGAGATCCCGGCCCGTGACGGCGT
  CCTTTTCCTCAAGCTCTACGATGGCTGGACCGAGCCCTTCGCCTTCCACATGCGCAAGTCGCTCTACGGC
  TTCTTCTGGAAGCAGCCGGGCACCCTGGAGGTGGTGTCAGGCTGCACGGTGGACATGCTGCAGGCAGTGT
  ATGGGCTGGACGGCATCCGCCTGCGCCGCCGCCAGTACTACACCATGCCCAACTTCAGACAGTATGAGAA
  CCGCACCGGCCACATCCTGGTGCAGTGGTCGCTGGGCAGCCCCCTGCACTTCGCCGGCTGGCACTGCTCC
  TGGTGCTTCACGCCCGAGGGCATCTACTTCAAGCTCGTGTCCGCCCAGAATGGCGACTTCCCACGCTGGG
  GTGACTACGAGGACAAGCGGGACCTGAACTACATCCGCGGCCTGATCCGCACCGGGGGCTGGTTCGACGG
  CACGCAGCAGGAGTACCCGCCTGCAGACCCCAGCGAGCACATGTATGCGCCCAAGTACCTGCTGAAGAAC
  TACGACCGGTTCCACTACCTGCTGGACAACCCCTACCAGGAGCCCAGGAGCACGGCGGCGGGCGGGTGGC
  GCCACAGGGGTCCCGAGGGAAGGCCGCCCGCCCGGGGCAAACTGGACGAGGCGGAAGTCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098270
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098270.1, NP_001091740.1
• RefSeq Size: 4987 bp
• RefSeq ORF: 1602 bp
• Locus ID: 4248
• Cytogenetics: 22q13.1
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1, and is transcribed from the H204 promoter. Both variants 1 and 2 encode the same protein.