TCF4 (NM_001083962) Human Untagged Clone

CAT#: SC316252

TCF4 (untagged)-Human transcription factor 4 (TCF4), transcript variant 1


  "NM_001083962" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TCF4
Synonyms bHLHb19; E2-2; FECD3; ITF-2; ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001083962 edited
ATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACC
GTGGAGTTGCCCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCT
GATCAACACAGGTCTGTTTCTGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCA
TTCGGACGTGTGAAAAGAAGCTTGCTTCACATTGCAGCAAATTGTGGATCGGTGGAATGC
TTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATCAAGATATTTCAGGCTGTACA
CCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATTATTAGAATAT
AGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTG
AATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAG
GATGCCATGGGGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTG
CAGTGCTTGCTAGACAGTGGTGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCA
TTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACAGCACAGATCCTACTATTACGAGGA
GCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGGATTTATGTGTACAGGGTGGA
TATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCAGACTATTATT
CAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTG
TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACA
ACAAATGGTCATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTT
TTAAGGATTGTGAGAATGTTTTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAAT
GGAATTGATATGGGCTACAATGGGAATAAAACTCCAAGAAGCCAGGTGTTCAAGCCTCTG
GAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAATAGCCACAGAATTGATGAAA
AACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGGCCAAGATCAA
GATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCC
ACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGAT
TGTCAGGATGTTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTAC
ATGTGCTGTTCTTGTCAGATGCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTC
TGCAAACATGATGAAGTTTTAAAATGCTTTGTTAATAGAAATCCCAAAATTATATTTGAC
CACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAAGATTCATGCATATCATAAAA
GCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTCAGGACAGCCA
GATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGAT
TCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAA
GGGATTGCTGTACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGG
TTTGATATTCTGTCCAATGAGATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCT
GATGGAACAACTTTTCAGCCTAATAGCAACTCTTATGTAAATCCTGATCACTTGAACTAT
TTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACCACAGGCAGCTGGTCAATATT
TACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTACCAAGATGTG
GCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGT
GATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAG
GTGCCTTTGAAACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTAC
GTCCAGCTTGTTACTGAACTTCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTT
TTACAGGGCTTTCATATGTTCATTCCACCCTCCCTCATACAGCTTTTTGATGAATATGAA
TTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGAGTGATTGGATAAAAAATACA
GAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTGGGAAGTTGTA
GAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGG
GTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATC
GCTGCTGTGCCATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTC
AAGTTACCTGAATACCCAAGTAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACAT
TGTGGCAGCTATGGTTACACAATGGCAACGCGTACGCGGCCGCTCGAGCAGAAACTCATC
TCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTT
TAAAC
Restriction Sites Please inquire     
ACCN NM_001083962
Insert Size 2200 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation ORF sequence matches with the reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001083962.1, NP_001077431.1
RefSeq Size 8332 bp
RefSeq ORF 2016 bp
Locus ID 6925
Cytogenetics 18q21.2
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Gene Summary 'This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016]'
Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (a, also known as TCF4-B+) is shorter at the N-terminus compared to isoform c. Sequence Note: This gene is distinct from TCF7L2 (alias TCF-4).

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