HIC1 (NM_001098202) Human Untagged Clone

CAT#: SC316257

HIC1 (untagged)-Human hypermethylated in cancer 1 (HIC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIC1
• Synonyms: hic-1; ZBTB29; ZNF901
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001098202, the custom clone sequence may differ by one or more nucleotides
  ATGACTTTTCCTGAAGCGGACATTTTACTTAAATCGGGAGAGTGTGCTGGGCAGACGATG
  CTGGACACGATGGAGGCGCCCGGCCACTCCAGGCAGCTGCTGCTGCAGCTCAACAACCAG
  CGCACCAAGGGCTTCTTGTGCGACGTGATCATCGTGGTGCAGAACGCCCTCTTCCGCGCG
  CACAAGAACGTGCTGGCGGCCAGCAGCGCCTACCTCAAGTCCCTGGTGGTGCATGACAAC
  CTGCTCAACCTGGACCATGACATGGTGAGCCCGGCCGTGTTCCGCCTGGTGCTGGACTTC
  ATCTACACCGGCCGCCTGGCTGACGGCGCAGAGGCGGCTGCGGCCGCGGCCGTGGCCCCG
  GGGGCTGAGCCGAGCCTGGGCGCCGTGCTGGCCGCCGCCAGCTACCTGCAGATCCCCGAC
  CTCGTGGCGCTGTGCAAGAAACGCCTCAAGCGCCACGGCAAGTACTGCCACCTGCGGGGC
  GGCGGCGGCGGCGGCGGCGGCTACGCGCCCTATGGTCGGCCGGGCCGGGGCCTGCGGGCC
  GCCACGCCGGTCATCCAGGCCTGCTACCCGTCCCCAGTCGGGCCTCCGCCGCCGCCTGCC
  GCGGAGCCGCCCTCGGGCCCAGAGGCCGCGGTCAACACGCACTGCGCCGAGCTGTACGCG
  TCGGGACCCGGCCCGGCCGCCGCACTCTGTGCCTCGGAGCGCCGCTGCTCCCCTCTTTGT
  GGCCTGGACCTGTCCAAGAAGAGCCCGCCGGGCTCCGCGGCGCCAGAGCGGCCGCTGGCT
  GAGCGCGAGCTGCCCCCGCGCCCGGACAGCCCTCCCAGCGCCGGCCCCGCCGCCTACAAG
  GAGCCGCCTCTCGCCCTGCCGTCGCTGCCGCCGCTGCCCTTCCAGAAGCTGGAGGAGGCC
  GCACCGCCTTCCGACCCATTTCGCGGCGGCAGCGGCAGCCCGGGACCCGAGCCCCCCGGC
  CGCCCCGACGGGCCTAGTCTCCTCTATCGCTGGATGAAGCACGAGCCGGGCCTGGGTAGC
  TATGGCGACGAGCTGGGCCGGGAGCGCGGCTCCCCCAGCGAGCGCTGCGAAGAGCGTGGT
  GGGGACGCGGCCGTCTCGCCCGGGGGGCCCCCGCTCGGCCTGGCGCCGCCGCCGCGCTAC
  CCTGGCAGCCTGGACGGGCCCGGCGCGGGCGGCGACGGCGACGACTACAAGAGCAGCAGC
  GAGGAGACCGGTAGCAGCGAGGACCCCAGCCCGCCTGGCGGCCACCTCGAGGGCTACCCA
  TGCCCGCACCTGGCCTATGGCGAGCCCGAGAGCTTCGGTGACAACCTGTACGTGTGCATT
  CCGTGCGGCAAGGGCTTCCCCAGCTCTGAGCAGCTGAACGCGCACGTGGAGGCTCACGTG
  GAGGAGGAGGAAGCGCTGTACGGCAGGGCCGAGGCGGCCGAAGTGGCCGCTGGGGCCGCC
  GGCCTAGGGCCCCCTTTTGGAGGCGGCGGGGACAAGGTCGCCGGGGCTCCGGGTGGCCTG
  GGAGAGCTGCTGCGGCCCTACCGCTGCGCGTCGTGCGACAAGAGCTACAAGGACCCGGCC
  ACGCTGCGGCAGCACGAGAAGACGCACTGGCTGACCCGGCCCTACCCATGCACCATCTGC
  GGGAAGAAGTTCACGCAGCGTGGGACCATGACGCGCCACATGCGCAGCCACCTGGGCCTC
  AAGCCCTTCGCGTGCGACGCGTGCGGCATGCGGTTCACGCGCCAGTACCGCCTCACGGAG
  CACATGCGCATCCACTCGGGCGAGAAGCCCTACGAGTGCCAGGTGTGCGGCGGCAAGTTC
  GCACAGCAACGCAACCTCATCAGCCACATGAAGATGCACGCCGTGGGGGGCGCGGCCGGC
  GCGGCCGGGGCGCTGGCGGGCTTGGGGGGGCTCCCCGGCGTCCCCGGCCCCGACGGCAAG
  GGCAAGCTCGACTTCCCCGAGGGCGTCTTTGCTGTGGCTCGCCTCACGGCCGAGCAGCTG
  AGCCTGAAGCAGCAGGACAAGGCGGCCGCGGCCGAGCTGCTGGCGCAGACCACGCACTTC
  CTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACCCGCTGGCCAAGTTCACGGCCGAG
  CTGGGCCTCAGCCCCGACAAGGCGGCCGAGGTGCTGAGCCAGGGCGCTCACCTGGCGGCC
  GGGCCCGACGGCCGGACCATCGACCGTTTCTCTCCCACC
  
• Restriction Sites: Please inquire
• ACCN: NM_001098202
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098202.1, NP_001091672.1
• RefSeq Size: 3071 bp
• RefSeq ORF: 2202 bp
• Locus ID: 3090
• Cytogenetics: 17p13.3
• Gene Summary: 'This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]'
  Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region and uses a downstream start codon, compared to variant 1. Isoform 2 has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.