Miz1 (ZBTB17) (NM_003443) Human Untagged Clone

CAT#: SC316264

ZBTB17 (untagged)-Human zinc finger and BTB domain containing 17 (ZBTB17)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZBTB17
• Synonyms: MIZ-1; pHZ-67; ZNF60; ZNF151
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC316264 sequence for NM_003443 edited (data generated by NextGen Sequencing)
  ATGGACTTTCCCCAGCACAGCCAGCATGTCTTGGAACAGCTGAACCAGCAGCGGCAGCTG
  GGGCTTCTCTGTGACTGCACCTTTGTGGTGGACGGTGTTCACTTTAAGGCTCATAAAGCA
  GTGCTGGCGGCCTGCAGCGAGTACTTCAAGATGCTCTTCGTGGACCAGAAGGACGTGGTG
  CACCTGGACATCAGTAACGCGGCAGGCCTGGGGCAGGTGCTGGAGTTTATGTACACGGCC
  AAGCTGAGCCTGAGCCCTGAGAACGTGGATGATGTGCTGGCCGTGGCCACTTTCCTCCAA
  ATGCAGGACATCATCACGGCCTGCCATGCCCTCAAGTCACTTGCTGAGCCGGCTACCAGC
  CCTGGGGGAAATGCGGAGGCCTTGGCCACAGAAGGAGGGGACAAGAGAGCCAAAGAGGAG
  AAGGTGGCCACCAGCACGCTGAGCAGGCTGGAGCAGGCAGGACGCAGCACACCCATAGGC
  CCCAGCAGGGACCTCAAGGAGGAGCGCGGCGGTCAGGCCCAGAGTGCGGCCAGCGGTGCA
  GAGCAGACAGAGAAAGCCGATGCGCCCCGGGAGCCGCCGCCTGTGGAGCTCAAGCCAGAC
  CCCACGAGTGGCATGGCTGCTGCAGAAGCTGAGGCCGCTTTGTCCGAGAGCTCGGAGCAA
  GAAATGGAGGTGGAGCCCGCCCGGAAAGGGGAAGAGGAGCAAAAGGAGCAAGAGGAGCAA
  GAGGAGGAGGGCGCAGGGCCAGCTGAGGTCAAGGAGGAGGGTTCCCAGCTGGAGAACGGA
  GAGGCCCCCGAGGAGAACGAGAATGAGGAGTCAGCGGGCACAGACTCGGGGCAGGAGCTC
  GGCTCCGAGGCCCGGGGCCTGCGCTCAGGCACCTACGGCGACCGCACGGAGTCCAAGGCC
  TACGGCTCCGTCATCCACAAGTGCGAGGACTGTGGGAAGGAGTTCACGCACACGGGGAAC
  TTCAAGCGGCACATCCGCATCCACACGGGGGAGAAGCCCTTCTCGTGCCGGGAGTGCAGC
  AAGGCCTTTTCCGACCCGGCCGCGTGCAAGGCCCATGAGAAGACGCACAGCCCTCTGAAG
  CCCTACGGCTGCGAGGAGTGCGGGAAGAGCTACCGCCTCATCAGCCTGCTGAACCTGCAC
  AAGAAGCGGCACTCGGGCGAGGCGCGCTACCGCTGCGAGGACTGCGGCAAGCTCTTCACC
  ACCTCGGGCAACCTCAAGCGCCACCAGCTGGTGCACAGCGGCGAGAAGCCCTACCAGTGC
  GACTACTGCGGCCGCTCCTTCTCCGACCCCACTTCCAAGATGCGCCACCTGGAGACCCAC
  GACACGGACAAGGAGCACAAGTGCCCACACTGCGACAAGAAGTTCAACCAGGTAGGGAAC
  CTGAAGGCCCACCTGAAGATCCACATCGCTGACGGGCCCCTCAAGTGCCGAGAGTGTGGG
  AAGCAGTTCACCACCTCAGGGAACCTGAAGCGGCACCTTCGGATCCACAGCGGGGAGAAG
  CCCTACGTGTGCATCCACTGCCAGCGACAGTTTGCAGACCCCGGCGCTCTGCAGCGGCAC
  GTCCGCATTCACACAGGTGAGAAGCCATGCCAGTGTGTGATGTGCGGTAAGGCCTTCACC
  CAGGCCAGCTCCCTCATCGCCCACGTGCGCCAGCACACCGGGGAGAAGCCCTACGTCTGC
  GAGCGCTGCGGCAAGAGATTCGTCCAGTCCAGCCAGTTGGCCAATCATATTCGCCACCAC
  GACAACATCCGCCCACACAAGTGCAGCGTGTGCAGCAAGGCCTTCGTGAACGTGGGGGAC
  CTGTCCAAGCACATCATCATTCACACTGGAGAGAAGCCTTACCTGTGTGATAAGTGTGGG
  CGTGGCTTCAACCGGGTAGACAACCTGCGCTCCCACGTGAAGACCGTGCACCAGGGCAAG
  GCAGGCATCAAGATCCTGGAGCCCGAGGAGGGCAGTGAGGTCAGCGTGGTCACTGTGGAT
  GACATGGTCACGCTGGCTACCGAGGCACTGGCAGCGACAGCCGTCACTCAGCTCACAGTG
  GTGCCGGTGGGAGCTGCAGTGACAGCCGATGAGACGGAAGTCCTGAAGGCCGAGATCAGC
  AAAGCTGTGAAGCAAGTGCAGGAAGAAGACCCCAACACTCACATCCTCTACGCCTGTGAC
  TCCTGTGGGGACAAGTTTCTGGATGCCAACAGCCTGGCTCAGCATGTGCGAATCCACACA
  GCCCAGGCACTGGTCATGTTCCAGACAGACGCGGACTTCTATCAGCAGTATGGGCCAGGT
  GGCACGTGGCCTGCCGGGCAGGTGCTGCAGGCTGGGGAGCTGGTCTTCCGCCCTCGCGAC
  GGGGCTGAGGGCCAGCCCGCACTGGCAGAGACCTCCCCTACAGCTCCTGAATGTCCCCCG
  CCTGCCGAGTGA
  
  Clone variation with respect to NM_003443.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003443 unedited
  CGGCCGGCGATTCGGCACGAGGGAGCCGGACGAGGGGAGCCAAGCTGGAGTTTACACAGG
  CAAACTGTCAGAAAAGAGTAGCCTGGGCTGTCTGGAAATCTGAGCCATGGACTTTCCCCA
  GCACAGCCAGCATGTCTTGGAACAGCTGAACCAGCAGCGGCAGCTGGGGCTTCTCTGTGA
  CTGCACCTTTGTGGTGGACGGTGTTCACTTTAAGGCTCATAAAGCAGTGCTGGCGGCCTG
  CAGCGAGTACTTCAAGATGCTCTTCGTGGACCAGAAGGACGTGGTGCACCTGGACATCAG
  TAACGCGGCAGGCCTGGGGCAGGTGCTGGAGTTTATGTACACGGCCAAGCTGAGCCTGAG
  CCCTGAGAACGTGGATGATGTGCTGGCCGTGGCCACTTTCCTCCAAATGCAGGACATCAT
  CACGGCCTGCCATGCCCTCAAGTCACTTGCTGAGCCGGCTACCAGCCCTGGGGGAAATGC
  GGAGGCCTTGGCCACAGAAGGAGGGGACAAGAGAGCCAAAGAGGAGAAGGTGGCCACCAG
  CACGCTGAGCAGGCTGGAGCAGGCAGGACGCAGCACACCCATAGGCCCCAGCAGGGACCT
  CAAGGAGGAGCGCGGCGGTCAGGCCCAGAGTGCGGCCAGCGGTGCAGAGCAGACAGAGAA
  AGCCGATGNCGCCCCGGAGCCGCCGCCTGTGGAGCTCAGCCAGACCCCACGAGTGGCATG
  GCTGCTGCAGAGCTGAGGCCGCTTTGTCCGAGAGCCTCGAGCAGAAATGGNAGTGGAGCC
  CGCCCGGAAGGGGNAGAGGAGCAAAGGAGCCAGAGAGCAGAGGAGGAGGCGCAGGCCANC
  TGAGTAAGNNAGGAGGGTTCCANCTGGAAANCGGAAAGCN
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_003443 unedited
  TGGACGAATAGCCTTACCTGTGTGATAAGTGTGGGCGCTGGCTTCAACCGGGTAGACAAC
  CTGCGCTCCCACGTGAAGACCGTGCACCAGGGCAAGGCAGGCATCAAGATCCTGGAGCCC
  GAGGAGGGCAGTGAGGTCAGCGTGGTCACTGTGGATGACATGGTCACGCTGGCTACCGAG
  GCACTGGCAGCGACAGCCGTCACTCAGCTCACAGTGGTGCCGGTGGGAGCTGCAGTGACA
  GCCGATGAGACGGAAGTCCTGAAGGCCGAGATCAGCAAAGCTGTGAAGCAAGTGCAGGAA
  GAAGACCCCAACACTCACATCCTCTACGCCTGTGACTCCTGTGGGGACAAGTTTCTGGAT
  GCCAACAGCCTGGCTCAGCATGTGCGAATCCACACAGCCCAGGCACTGGTCATGTTCCAG
  ACAGACGCGGACTTCTATCAGCAGTATGGGCCAGGTGGCACGTGGCCTGCCGGGCAGGTG
  CTGCAGGCTGGGGAGCTGGTCTTCCGCCCTCGCGACGGGGCTGAGGGCCAGCCCGCACTG
  GCAGAGACCTCCCCTACAGCTCCTGAATGTCCCCCGCCTGCCGAGTGAGCTGGCGGCCCT
  TCTGACTGTTTATTTAAGGATGGATGGCACCCTGGAACCGGGAAGGGTGGCCTGTTCCCT
  AGAGAGAATAAATTGGATTATTTTCTATATAAAAAAAGAAACAAAAAATGTCAAAAAAAT
  AAAAAAAAAGAAAAAATAAACAAGAAGAACAAATAGAAACCACAAACACTAATCAATAAA
  TCATCAAGAGAAAGACCCCCTCTTCTTTTTTTTGTGCGCCCGGGTCAATGTTTTTTTCCA
  AAAAATATCCCGGGGGGGACTCCTGGGGACCCCCCCCCAGGGCCCTCCTCCGGGGCCCGG
  GAATGTGCCCTCTACTCGCGCCAAAACCTTTGCTAAAAAAAAAAAATGTCAAATTTTTGG
  TCAGACAGAGGGTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003443
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003443.1, NP_003434.1
• RefSeq Size: 2643 bp
• RefSeq ORF: 2412 bp
• Locus ID: 7709
• Cytogenetics: 1p36.13
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle
• Gene Summary: 'This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18. [provided by RefSeq, Jul 2008]'