SSB (NM_003142) Human Untagged Clone

CAT#: SC316315

SSB (untagged)-Human Sjogren syndrome antigen B (autoantigen La) (SSB)


  "NM_003142" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SSB
Synonyms La; La/SSB; LARP3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_003142 edited
CGTTGCTGTTGCTGTTTGTGAGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGAT
AGCCGCAATGGCTGAAAATGGTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTG
TCATCAAATTGAGTATTATTTTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGA
ACAGATAAAACTGGATGAAGGCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTT
GAACCGTCTAACAACAGACTTTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGA
ACTCATGGAAATCAGTGAAGATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACC
TGAAGTGACTGATGAGTATAAAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTT
CCCAACTGATGCAACTCTTGATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACT
AAATATTCAGATGAGAAGAACATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTT
TGATAGCATTGAATCTGCTAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAAC
AGACCTGCTAATACTTTTCAAGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACA
AAATAAAGTGGAAGCTAAATTAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAAGTTAGA
AGAAGATGCTGAAATGAAATCTCTAGAAGAAAAGATTGGATGCTTGCTGAAATTTTCGGG
TGATTTAGATGATCAGACCTGTAGAGAAGATTTACACATACTTTTCTCAAATCATGGTGA
AATAAAATGGATAGACTTCGTCAGAGGAGCAAAAGAGGGGATAATTCTATTTAAAGAAAA
AGCCAAGGAAGCATTGGGTAAAGCCAAAGATGCAAATAATGGTAACCTACAATTAAGGAA
CAAAGAAGTGACTTGGGAAGTACTAGAAGGAGAGGTGGAAAAAGAAGCACTGAAGAAAAT
AATAGAAGACCAACAAGAATCCCTAAACAAATGGAAGTCAAAAGGTCGTAGATTTAAAGG
AAAAGGAAAGGGTAATAAAGCTGCCCAGCCTGGGTCTGGTAAAGGAAAAGTACAGTTTCA
GGGCAAGAAAACGAAATTTGCTAGTGATGATGAACATGATGAACATGATGAAAATGGTGC
AACTGGACCTGTGAAAAGAGCAAGAGAAGAAACAGACAAAGAAGAACCTGCATCCAAACA
ACAGAAAACAGAAAATGGTGCTGGAGACCAGTAGTTTAGTAAACCAATYTTTTATTCATT
TTAAATAGGTTTTAAACGACTTTTGTTTGCGGGGCTTTTAAAAGGAAAACCGAATTAGGT
CCACTTCAATGTCCACCTGTGAGAAAGGAAAAATTTTTTTGTTGTTTAACTTGTCTTTTT
GTTATGCAAATGAGATTTCTTTGAATGTATTGTTCTGTTTGTGTTATTTCAGATGATTCA
AATATCAAAAGGAAGATTCTTCCATTAAATTGCCTTTGTAATATGAGAATGTATTAGTAC
AAACTAACTAATAAAATATATACTATATGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAA
>OriGene 5' read for NM_003142 unedited
TAATACGACTCACTATAGGGCGGCCGCGACATTCGCACGAGGCGTTGCTGTTGCTGTTTG
TGAGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAATGGCTGAAAA
TGGTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTGTCATCAAATTGAGTATTA
TTTTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGAACAGATAAAACTGGATGA
AGGCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTTGAACCGTCTAACAACAGA
CTTTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGAACTCATGGAAATCAGTGA
AGATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACCTGAAGTGACTGATGAGTA
TAAAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTTCCCAACTGATGCAACTCT
TGATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACTAAATATTCAGATGAGAAG
AACATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGC
TAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTT
CAAGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAA
ATTAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAGTTAGAAGAGATGCTGAAATGAAAT
CTCTAGAAGAAAGATNGACTGCTGCTGAAATTTCGGTGATTTAGAGATCAGACTGTAGAG
AAGATTACACATACTTTNTNAAATATGNTGAATAAATGGATGACCTTGTCAGAGAGCAAG
AGGGGATAATTCATTTAAAGAAAAGCAAGNNAGCTTGGGTAAGCCAAGATGCAATATGGT
ACCTCANTAA
>Forward primer walk for NM_003142 unedited
AACATGGTTAGAGAAAAGGTCAGTACTAAATACCTCAGATGAGAAGAACATTGCATAAAG
CATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGCTAAGAAATTTGTAG
AGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTTCAAGGACGATTACT
TTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAAATTAAGAGCTAAAC
AGGAGCAAGAAGCAAAACAAAAGTTAGAAGAAGATGCTGAAATGAAATCTCTAGAAGAAA
AGATTGGATGCTTGCTGAAATTTTCGGGTGATTTAGATGATCAGACCTGTAGAGAAGATT
TACACATACTTTTCTCAAATCATGGTGAAATAAAATGGATAGACTTCGTCAGAGGAGCAA
AAGAGGGGATAATTCTATTTAAAGAAAAAGCCAAGGAAGCATTGGGTAAAGCCAAAGATG
CAAATAATGGTAACCTACAATTAAGGAACAAAGAAGTGACTTGGGAAGTACTAGAAGGAG
AGGTGGAAAAAGAAGCACTGAAGAAAATAATAGAAGACCAACAAGAATCCCTAAACAAAT
GGAAGTCAAAAGGTCGTAGATTTAAAGGAAAAGGAAAGGGTAATAAAGCTGCCCAGCCTG
GGTCTGGTAAAGGAAAAGTACAGTTTCAGGGCAAGAAAACGAAATTTGCTAGTGATGATG
AACATGATGAACATGATGAAAATGGTGCAACTGGACCTGTGAAAAGAGCAAGAGAGAACA
GACAAAGAAGACCCTGCATCCAAACAACAGAAAACAGAAATGGTGCTGAGACAGTAGTTT
AGTAAACCAATTTTTATTCATTTTAAATAGTTTTAAACGACTTTTGTTGCGGGCTTTAAG
AAACGATAGTCACTCATGTCCACCTGTGAGAAAGAAATTTTTTTGTTGTTTAACTTGTTC
TTTTTGTTATGCA
Restriction Sites NotI-NotI     
ACCN NM_003142
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003142.3, NP_003133.1
RefSeq Size 1673 bp
RefSeq ORF 1227 bp
Locus ID 6741
Cytogenetics 2q31.1
Domains RRM, LA
Protein Families Stem cell - Pluripotency, Transcription Factors
Protein Pathways Systemic lupus erythematosus
Gene Summary 'The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]'
Transcript Variant: This variant (1) and variant 2 encode the same protein.

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