Integrin alpha E (ITGAE) (NM_002208) Human Untagged Clone

CAT#: SC316319

ITGAE (untagged)-Human integrin, alpha E (antigen CD103, Human mucosal lymphocyte antigen 1, alpha polypeptide) (ITGAE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITGAE
• Synonyms: CD103; HUMINAE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002208 edited
  AAGGATGTGGCTCTTCCACACTCTGCTCTGCATAGCCAGCCTGGCCCTGCTGGCCGCTTT
  CAATGTGGATGTGGCCCGGCCCTGGCTCACGCCCAAGGGAGGTGCCCCTTTCGTGCTCAG
  CTCCCTTCTGCACCAAGACCCCAGCACCAACCAGACCTGGCTCCTGGTCACCAGCCCCAG
  AACCAAGAGGACACCAGGGCCCCTCCATCGATGTTCCCTTGTCCAGGATGAAATCCTTTG
  CCATCCTGTAGAGCATGTCCCCATCCCCAAGGGGAGGCACCGGGGAGTGACCGTTGTCCG
  GAGCCACCACGGTGTTTTGATATGCATTCAAGTGCTGGTCCGGCGGCCTCACAGCCTCAG
  CTCAGAACTCACAGGCACCTGTAGCCTCCTGGGCCCTGACCTCCGTCCCCAGGCTCAGGC
  CAACTTCTTCGACCTTGAAAATCTCCTGGATCCAGATGCACGTGTGGACACTGGAGACTG
  CTACAGCAACAAAGAAGGCGGTGGAGAAGACGATGTGAACACAGCCAGGCAGCGCCGGGC
  TCTGGAGAAGGAGGAGGAGGAAGACAAGGAGGAGGAGGAAGACGAGGAGGAGGAGGAAGC
  TGGCACCGAGATTGCCATCATCCTGGATGGCTCAGGAAGCATTGATCCCCCAGACTTTCA
  GAGAGCCAAAGACTTCATCTCCAACATGATGAGGAACTTCTATGAAAAGTGTTTTGAGTG
  CAACTTTGCCTTGGTGCAGTATGGAGGAGTGATCCAGACTGAGTTTGACCTTCGGGACAG
  CCAGGATGTGATGGCCTCCCTCGCCAGAGTCCAGAACATCACTCAAGTGGGGAGTGTCAC
  CAAGACTGCCTCAGCCATGCAACACGTCTTAGACAGCATCTTCACCTCAAGCCACGGCTC
  CAGGAGAAAGGCATCCAAGGTCATGGTGGTGCTCACCGATGGTGGCATATTCGAGGACCC
  CCTCAACCTTACGACAGTCATCAACTCCCCCAAAATGCAGGGTGTTGAGCGCTTTGCCAT
  TGGGGTGGGAGAAGAATTTAAGAGTGCTAGGACTGCGAGGGAACTGAACCTGATCGCCTC
  AGACCCGGATGAGACCCATGCTTTCAAGGTGACCAACTACATGGCGCTGGATGGGCTGCT
  GAGCAAACTGCGGTACAACATCATCAGCATGGAAGGCACGGTTGGAGACGCCCTTCACTA
  CCAGCTGGCACAGATTGGCTTCAGTGCTCAGATCCTGGATGAGCGGCAGGTGCTGCTCGG
  CGCCGTCGGGGCCTTTGACTGGTCCGGAGGGGCGTTGCTCTACGACACACGCAGCCGCCG
  GGGCCGCTTCCTGAACCAGACAGCGGCGGCGGCGGCAGACGCGGAGGCTGCGCAGTACAG
  CTACCTGGGTTACGCTGTGGCCGTGCTGCACAAGACCTGCAGCCTCTCCTACGTCGCGGG
  GGCTCCACAGTACAAACATCATGGGGCCGTGTTTGAGCTCCAGAAGGAGGGCAGAGAGGC
  CAGCTTCCTGCCAGTGCTGGAGGGAGAGCAGATGGGGTCCTATTTTGGCTCTGAGCTGTG
  CCCTGTGGACATTGACATGGATGGAAGCACGGACTTCTTGCTGGTGGCTGCTCCATTTTA
  CCACGTTCATGGAGAAGAAGGCAGAGTCTACGTGTACCGTCTCAGCGAGCAGGATGGTTC
  TTTCTCCTTGGCACGCATACTGAGTGGGCACCCCGGGTTCACCAATGCCCGCTTTGGCTT
  TGCCATGGCGGCTATGGGGGATCTCAGTCAGGATAAGCTCACAGATGTGGCCATCGGGGC
  CCCCCTGGAAGGTTTTGGGGCAGATGATGGTGCCAGCTTCGGCAGTGTGTATATCTACAA
  TGGACACTGGGACGGCCTCTCCGCCAGCCCCTCGCAGCGGATCAGAGCCTCCACGGTGGC
  CCCAGGACTCCAGTACTTCGGCATGTCCATGGCTGGTGGCTTTGATATTAGTGGCGACGG
  CCTTGCCGACATCACCGTGGGCACTCTGGGCCAGGCGGTTGTGTTCCGCTCCCGGCCTGT
  GGTTCGCCTGAAGGTCTCCATGGCCTTCACCCCCAGCGCACTGCCCATCGGCTTCAACGG
  CGTCGTGAATGTCCGTTTATGTTTTGAAATCAGCTCTGTAACCACAGCCTCTGAGTCAGG
  CCTCCGTGAGGCACTTCTCAACTTCACGCTGGATGTGGATGTGGGGAAGCAGAGGAGACG
  GCTGCAGTGTTCAGACGTAAGAAGCTGTCTGGGCTGCCTGAGGGAGTGGAGCAGCGGATC
  CCAGCTTTGTGAGGACCTCCTGCTCATGCCCACAGAGGGAGAGCTCTGTGAGGAGGACTG
  CTTCTCCAATGCCAGTGTCAAAGTCAGCTACCAGCTCCAGACCCCTGAGGGACAGACGGA
  CCATCCCCAGCCCATCCTGGACCGCTACACTGAGCCCTTTGCCATCTTCCAGCTGCCCTA
  TGAGAAGGCCTGCAAGAATAAGCTGTTTTGTGTCGCAGAATTACAGTTGGCCACCACCGT
  CTCTCAGCAGGAGTTGGTGGTGGGTCTCACAAAGGAGCTGACCCTGAACATTAACCTAAC
  TAACTCCGGGGAAGATTCCTACATGACAAGCATGGCCTTGAATTACCCCAGAAACCTGCA
  GTTGAAGAGGATGCAAAAGCCTCCCTCTCCAAACATTCAGTGTGATGACCCTCAGCCGGT
  TGCTTCTGTCCTGATCATGAACTGCAGGATTGGTCACCCCGTCCTCAAGAGGTCATCTGC
  TCATGTTTCAGTCGTTTGGCAGCTAGAGGAGAATGCCTTTCCAAACAGGACAGCAGACAT
  CACTGTGACTGTCACCAATTCCAATGAAAGACGGTCTTTGGCCAACGAGACCCACACCCT
  TCAATTCAGGCATGGCTTCGTTGCAGTTCTGTCCAAACCATCCATAATGTACGTGAACAC
  AGGCCAGGGGCTTTCTCACCACAAAGAATTCCTCTTCCATGTACATGGGGAGAACCTCTT
  TGGAGCAGAATACCAGTTGCAAATTTGCGTCCCAACCAAATTACGAGGTCTCCAGGTTGT
  AGCAGTGAAGAAGCTGACGAGGACTCAGGCCTCCACGGTGTGCACCTGGAGTCAGGAGCG
  CGCTTGTGCGTACAGTTCGGTTCAGCATGTGGAAGAATGGCATTCAGTGAGCTGTGTCAT
  CGCTTCAGATAAAGAAAATGTCACCGTGGCTGCAGAGATCTCCTGGGATCACTCTGAGGA
  GTTACTAAAAGATGTAACTGAACTGCAGATCCTTGGTGAAATATCTTTCAACAAATCTCT
  ATATGAGGGACTGAATGCAGAGAACCACAGAACTAAGATCACTGTCGTCTTCCTGAAAGA
  TGAGAAGTACCATTCTTTGCCTATCATCATTAAAGGCAGCGTTGGTGGACTTCTGGTGTT
  GATCGTGATTCTGGTCATCCTGTTCAAGTGTGGCTTTTTTAAAAGAAAATATCAACAACT
  GAACTTGGAGAGCATCAGGAAGGCCCAGCTGAAATCAGAGAATCTGCTCGAAGAAGAGAA
  TTAGGACCTGCTATCCACTGGGAGAGGCTATCAGCCAGTCCTGGGACTTGGAGACCCAGC
  ATCCTTTGCATTACTTTTTCCTTCAGGATGATCTAGAGCAGCATGGAGCTGTTGGTAGAA
  TATTAGTTTTTAACCATACATTGTCCCAAAAGTGTCTGTGCATTGTGCAAAAAGTAAACT
  TAGGAAACATTTGGTATTAAATAAATTTACACTTTTCTTTGCAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002208 unedited
  GACGCCAATTTTGTTATACCGACTCCTATAGGGCGGCCGCTGTTATTCAGATCTGGTACC
  GAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCCAGGAATTCA
  AGGATGTGGCTCTTCCACACTCTGCTCTGCATAGCCAGCCTGGCCCTGCTGGCCGCTTTC
  AATGTGGATGTGGCCCGGCCCTGGCTCACGCCCAAGGGAGGTGCCCCTTTCGTGCTCAGC
  TCCCTTCTGCACCAAGACCCCAGCACCAACCAGACCTGGCTCCTGGTCACCAGCCCCAGA
  ACCAAGAGGACACCAGGGCCCCTCCATCGATGTTCCCTTGTCCAGGATGAAATCCTTTGC
  CATCCTGTAGAGCATGTCCCCATCCCCAAGGGGAGGCACCGGGGAGTGACCGTTGTCCGG
  AGCCACCACGGTGTTTTGATATGCATTCAAGTGCTGGTCCGGCGGCCTCACAGCCTCAGC
  TCAGAACTCACAGGCACCTGTAGCCTCCTGGGCCCTGACCTCCGTCCCCAGGCTCAGGCC
  AACTTCTTCGACCTTGAAAATCTCCTGGATCCAGATGCACGTGTGGACACTGGAGACTGC
  TACAGCAACAAAGAAGGCGGTGGAGAAGACGATGTGAACACAGCCAGGCAGCGCCGGGCT
  CTGGAGAAGGAGGAGGAGGAAGACAATGAGGAGGAGGAAGACGAGGAGGAGGAGGAAGCT
  GGCACCGAGATTGCCATCATCCTGGATGGCTCAGGAAGCATTGATCCCCCAGACTTTCAG
  AGAGCCAAAGACTTCATCTCCAACATGATGAGGACTTCTATGAAAGTGTTTGGAGTGCAA
  CTTTGCCTTGGTGCAGTATGAGAGTGATCAAACTGAGTTTGACCTCCGGACAGCCAGAAT
  GTGATGGCCTCCCTCGCCGAGTCAGACATCACTCAGTGGGGAAGTGCACCAAGAACTGCC
  TAGCATGCAACACGTCTA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_002208 unedited
  GTAACTCAGCCTACGCTAAATGATAGTATGCAGTATTCCTCCCTCTCCAAACATTCAGTG
  TGATGACCCTCAGCCGGTTGCTTCTGTCCTGATCATGAACTGCAGGATTGGTCACCCCGT
  CCTCAAGAGGTCATCTGCTCATGTTTCAGTCGTTTGGCAGCTAGAGGAGAATGCCTTTCC
  AAACAGGACAGCAGACATCACTGTGACTGTCACCAATTCCAATGAAAGACGGTCTTTGGC
  CAACGAGACCCACACCCTTCAATTCAGGCATGGCTTCGTTGCAGTTCTGTCCAAACCATC
  CATAATGTACGTGAACACAGGCCAGGGGCTTTCTCACCACAAAGAATTCCTCTTCCATGT
  ACATGGGGAGAACCTCTTTGGAGCAGAATACCAGTTGCAAATTTGCGTCCCAACCAAATT
  ACGAGGTCTCCAGGTTGTAGCAGTGAAGAAGCTGACGAGGACTCAGGCCTCCACGGTGTG
  CACCTGGAGTCAGGAGCGCGCTTGTGCGTACAGTTCGGTTCAGCATGTGGAAGAATGGCA
  TTCAGTGAGCTGTGTCATCGCTTCAGATAAAGAAAATGTCACCGTGGCTGCAGAGATCTC
  CTGGGATCACTCTGAGGAGTTACTAAAAGATGTAACTGAACTGCAGATCCTTGGTGAAAT
  ATCTTTCAACAAATCTCTATATGAGGGACTGAATGCAGAGAACCACAGAACTAAGATCAC
  TGTCGTCTTCCTGAAAGATGAGAAGTACCATTCTTTGCCTATCATCATTAAAGCAGCGTT
  GGTGGACTTCTGGTGTTGATCGTGATTCTGGTCATCCTGTTCAAGTGTGGCTTTTTTAAA
  AGAAAATATCAACAACTGAACTTGAGAGCATCAGAAGCCCAGCTGAATCAGAGATCTGCT
  CGAGAGAGATTAGACCTGCTATCCACTGGGGAGAAGCTATTCAGCCAGTTCTTGGGACTT
  GGACA
  
• Restriction Sites: Please inquire
• ACCN: NM_002208
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002208.4, NP_002199.3
• RefSeq Size: 3878 bp
• RefSeq ORF: 3540 bp
• Locus ID: 3682
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: 'Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]'