IRF5 (NM_001098629) Human Untagged Clone

CAT#: SC316398

IRF5 (untagged)-Human interferon regulatory factor 5 (IRF5), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRF5
• Synonyms: SLEB10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001098629 edited
  ATGAACCAGTCCATCCCAGTGGCTCCCACCCCACCCCGCCGCGTGCGGCTGAAGCCCTGG
  CTGGTGGCCCAGGTGAACAGCTGCCAGTACCCAGGGCTTCAATGGGTCAACGGGGAAAAG
  AAATTATTCTGCATCCCCTGGAGGCATGCCACAAGGCATGGTCCCAGCCAGGACGGAGAT
  AACACCATCTTCAAGGCCTGGGCCAAGGAGACAGGGAAATACACCGAAGGCGTGGATGAA
  GCCGATCCGGCCAAGTGGAAGGCCAACCTGCGCTGTGCCCTTAACAAGAGCCGGGACTTC
  CGCCTCATCTACGACGGGCCCCGGGACATGCCACCTCAGCCCTACAAGATCTACGAGGTC
  TGCTCCAATGGCCCTGCTCCCACAGACTCCCAGCCCCCTGAGGATTACTCTTTTGGTGCA
  GGAGAGGAGGAGGAAGAAGAGGAAGAGCTGCAGAGGATGTTGCCAAGCCTGAGCCTCACA
  GATGCAGTGCAGTCTGGCCCCCACATGACACCCTATTCTTTACTCAAAGAGGATGTCAAG
  TGGCCGCCCACTCTGCAGCCGCCCACTCTGCGGCCGCCTACTCTGCAGCCGCCCACTCTG
  CAGCCGCCCGTGGTGCTGGGTCCCCCTGCTCCAGACCCCAGCCCCCTGGCTCCTCCCCCT
  GGCAACCCTGCTGGCTTCAGGGAGCTTCTCTCTGAGGTCCTGGAGCCTGGGCCCCTGCCT
  GCCAGCCTGCCCCCTGCAGGCGAACAGCTCCTGCCAGACCTGCTGATCAGCCCCCACATG
  CTGCCTCTGACCGACCTGGAGATCAAGTTTCAGTACCGGGGGCGGCCACCCCGGGCCCTC
  ACCATCAGCAACCCCCATGGCTGCCGGCTCTTCTACAGCCAGCTGGAGGCCACCCAGGAG
  CAGGTGGAACTCTTCGGCCCCATAAGCCTGGAGCAAGTGCGCTTCCCCAGCCCTGAGGAC
  ATCCCCAGTGACAAGCAGCGCTTCTACACGAACCAGCTGCTGGATGTCCTGGACCGCGGG
  CTCATCCTCCAGCTACAGGGCCAGGACCTTTATGCCATCCGCCTGTGTCAGTGCAAGGTG
  TTCTGGAGCGGGCCTTGTGCCTCAGCCCATGACTCATGCCCCAACCCCATCCAGCGGGAG
  GTCAAGACCAAGCTTTTCAGCCTGGAGCATTTTCTCAATGAGCTCATCCTGTTCCAAAAG
  GGCCAGACCAACACCCCACCACCCTTCGAGATCTTCTTCTGCTTTGGGGAAGAATGGCCT
  GACCGCAAACCCCGAGAGAAGAAGCTCATTACTGTACAGGTGGTGCCTGTAGCAGCTCGA
  CTGCTGCTGGAGATGTTCTCAGGGGAGCTATCTTGGTCAGCTGATAGTATCCGGCTACAG
  ATCTCAAACCCAGACCTCAAAGACCGCATGGTGGAGCAATTCAAGGAGCTCCATCACATC
  TGGCAGTCCCAGCAGCGGTTGCAGCCTGTGGCCCAGGCCCCTCCTGGAGCAGGCCTTGGT
  GTTGGCCAGGGGCCCTGGCCTATGCACCCAGCTGGCATGCAATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001098629
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098629.1, NP_001092099.1
• RefSeq Size: 2900 bp
• RefSeq ORF: 1545 bp
• Locus ID: 3663
• Cytogenetics: 7q32.1
• Protein Families: Transcription Factors
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: 'This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 9. Variants 5 and 9 encode the same isoform.