cGKI (PRKG1) (NM_001098512) Human Untagged Clone

CAT#: SC316416

PRKG1 (untagged)-Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKG1
• Synonyms: AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001098512 edited
  ATGAGCGAGCTAGAGGAAGACTTTGCCAAGATTCTCATGCTCAAGGAGGAGAGGATCAAA
  GAGCTGGAGAAGCGGCTGTCAGAGAAGGAGGAAGAAATTCAGGAGCTGAAGAGGAAACTC
  CACAAATGCCAGTCGGTGCTCCCAGTGCCCTCGACCCACATCGGCCCCCGGACCACCCGG
  GCGCAGGGCATCTCGGCCGAGCCGCAGACGTACAGGTCCTTCCACGACCTCCGACAGGCA
  TTCCGGAAGTTCACCAAGTCCGAAAGGTCCAAGGATCTTATAAAGGAAGCTATCCTTGAC
  AATGACTTTATGAAGAACTTGGAGCTGTCGCAGATCCAGGAGATTGTGGATTGTATGTAC
  CCGGTGGAGTATGGCAAGGACAGTTGCATCATCAAAGAAGGAGACGTGGGGTCACTGGTG
  TATGTCATGGAAGATGGTAAGGTTGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATG
  GGTCCAGGAAAAGTGTTTGGGGAATTGGCTATTCTTTACAACTGTACCCGGACAGCGACC
  GTCAAGACTCTTGTAAATGTAAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATA
  ATGATGAGGACAGGACTCATCAAGCATACCGAGTATATGGAATTTTTAAAAAGCGTTCCA
  ACATTCCAGAGCCTTCCTGAAGAGATCCTCAGCAAGCTTGCTGATGTCCTTGAAGAGACC
  CACTATGAAAATGGAGAATATATTATCAGGCAAGGTGCAAGAGGGGACACCTTCTTTATC
  ATCAGCAAAGGAACGGTAAATGTCACTCGTGAAGACTCACCGAGTGAAGACCCAGTCTTT
  CTTAGAACTTTAGGAAAAGGAGACTGGTTTGGAGAGAAAGCCTTGCAGGGGGAAGATGTG
  AGAACAGCAAACGTAATTGCTGCAGAAGCTGTAACCTGCCTTGTGATTGACAGAGACTCT
  TTTAAACATTTGATTGGAGGGCTGGATGATGTTTCTAATAAAGCATATGAAGATGCAGAA
  GCTAAAGCAAAATATGAAGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCTGTCTGATTTC
  AACATCATTGATACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAACTGGTCCAGTTGAAA
  AGTGAAGAATCCAAAACGTTTGCAATGAAGATTCTCAAGAAACGTCACATTGTGGACACA
  AGACAGCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTC
  ATAGTGAGACTGTACAGAACATTTAAGGACAGCAAATATTTGTATATGTTGATGGAAGCT
  TGTCTAGGTGGAGAGCTCTGGACCATTCTCAGGGATAGAGGTTCGTTTGAAGATTCTACA
  ACCAGATTTTACACAGCATGTGTGGTAGAAGCTTTTGCCTATCTGCATTCCAAAGGAATC
  ATTTACAGGGACCTCAAGCCAGAAAATCTCATCCTAGATCACCGAGGTTATGCCAAACTG
  GTTGATTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGG
  ACTCCAGAGTATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCCGAC
  TACTGGTCACTGGGAATCCTAATGTATGAACTCCTGACTGGCAGCCCACCTTTCTCAGGC
  CCAGATCCTATGAAAACCTATAACATCATATTGAGGGGGATTGACATGATAGAATTTCCA
  AAGAAGATTGCCAAAAATGCTGCTAATTTAATTAAAAAACTATGCAGGGACAATCCATCA
  GAAAGATTAGGGAATTTGAAAAATGGAGTAAAAGACATTCAAAAGCACAAATGGTTTGAG
  GGCTTTAACTGGGAAGGCTTAAGAAAAGGTACCTTGACACCTCCTATAATACCAAGTGTT
  GCATCACCCACAGACACAAGTAATTTTGACAGTTTCCCTGAGGACAACGATGAACCACCA
  CCTGATGACAACTCAGGATGGGATATAGACTTCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001098512
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098512.1, NP_001091982.1
• RefSeq Size: 3824 bp
• RefSeq ORF: 2016 bp
• Locus ID: 5592
• Cytogenetics: 10q11.23-q21.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction, Vascular smooth muscle contraction
• Gene Summary: 'Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) encodes the shorter isoform (1; also known as alpha or 1alpha).