USP5 (NM_001098536) Human Untagged Clone

CAT#: SC316428

USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP5
• Synonyms: ISOT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001098536, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACC
  GGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGTAT
  GAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTAC
  TTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCCCGGA
  AGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAGAAGTTTGAATTAGA
  CGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGACTGCCT
  GACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGC
  AGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGA
  CAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTC
  AACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTG
  TGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGAGCTGA
  CGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGC
  ATCGACATGCTGAAGATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGA
  TTGGTGAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTACACAGG
  CATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTC
  CAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGACCCTACCCAGGATTTCA
  GCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCCAAGCCAGTACCGGAGTCGGG
  CGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGATGGCATTGCCCCTCGGATGTTCAAGGCCCTC
  ATCGGCAAGGGCCACCCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTA
  TCAACATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTGGAGGA
  AAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTACATCATGCAGCTGCCT
  GTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAGGAGAAGAAGCGGCAAGCCGAAG
  AGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCTTGCCTGGAGGCCTACGG
  GGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTGCAGGCCAAGTCAGTAGCTGTCAAGACCACA
  CGATTTGCCTCATTCCCTGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGC
  CCAAGAAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACAGGGCT
  GCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC
  CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCGACATCGCCCA
  TGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTCCCTATGGACGCCTGCCGCAAAGCTGT
  CTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAACTGGGTCATGTCACACATGGATGATCCAGAT
  TTTGCAAACCCCCTCATCCTGCCTGGCTCTAGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCTC
  CTGAGGACTGTGTGACCACCATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGGC
  CACGAACAATAGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCTGAAGCT
  GCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTGCCAGTGGGACCTAAAG
  TCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACCTCTACCATGTGTGG
  TCACTACGTCTGCCACATCAAGAAAGAAGGCAGATGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCC
  GAGAAGCCGCCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGCCAGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098536
• ORF Size: 2577 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001098536.1, NP_001092006.1
• RefSeq Size: 3214
• RefSeq ORF: 2577
• Locus ID: 8078
• Protein Families: Druggable Genome, Protease
• Gene Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]). [supplied by OMIM, Mar 2010]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).