CD166 (ALCAM) (NM_001627) Human Untagged Clone

CAT#: SC316550

ALCAM (untagged)-Human activated leukocyte cell adhesion molecule (ALCAM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALCAM
• Synonyms: CD166; MEMD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001627 edited
  ATGGAATCCAAGGGGGCCAGTTCCTGCCGTCTGCTCTTCTGCCTCTTGATCTCCGCCACC
  GTCTTCAGGCCAGGCCTTGGATGGTATACTGTAAATTCAGCATATGGAGATACCATTATC
  ATACCTTGCCGACTTGACGTACCTCAGAATCTCATGTTTGGCAAATGGAAATATGAAAAG
  CCCGATGGCTCCCCAGTATTTATTGCCTTCAGATCCTCTACAAAGAAAAGTGTGCAGTAC
  GACGATGTACCAGAATACAAAGACAGATTGAACCTCTCAGAAAACTACACTTTGTCTATC
  AGTAATGCAAGGATCAGTGATGAAAAGAGATTTGTGTGCATGCTAGTAACTGAGGACAAC
  GTGTTTGAGGCACCTACAATAGTCAAGGTGTTCAAGCAACCATCTAAACCTGAAATTGTA
  AGCAAAGCACTGTTTCTCGAAACAGAGCAGCTAAAAAAGTTGGGTGACTGCATTTCAGAA
  GACAGTTATCCAGATGGCAATATCACATGGTACAGGAATGGAAAAGTGCTACATCCCCTT
  GAAGGAGCGGTGGTCATAATTTTTAAAAAGGAAATGGACCCAGTGACTCAGCTCTATACC
  ATGACTTCCACCCTGGAGTACAAGACAACCAAGGCTGACATACAAATGCCATTCACCTGC
  TCGGTGACATATTATGGACCATCTGGCCAGAAAACAATTCATTCTGAACAGGCAGTATTT
  GATATTTACTATCCTACAGAGCAGGTGACAATACAAGTGCTGCCACCAAAAAGTGCCATC
  AAAGAAGGGGATAACATCACTCTTAAATGCTTAGGGAATGGCAACCCTCCCCCAGAGGAA
  TTTTTGTTTTACTTACCAGGACAGCCCGAAGGAATAAGAAGCTCAAATACTTACACACTG
  ACGGATGTGAGGCGCAATGCAACAGGAGACTACAAGTGTTCCCTGATAGACAAAAAAAGC
  ATGATTGCTTCAACAGCCATCACAGTTCACTATTTGGATTTGTCCTTAAACCCAAGTGGA
  GAAGTGACTAGACAGATTGGTGATGCCCTACCCGTGTCATGCACAATATCTGCTAGCAGG
  AATGCAACTGTGGTATGGATGAAAGATAACATCAGGCTTCGATCTAGCCCGTCATTTTCT
  AGTCTTCATTATCAGGATGCTGGAAACTATGTCTGCGAAACTGCTCTGCAGGAGGTTGAA
  GGACTAAAGAAAAGAGAGTCATTGACTCTCATTGTAGAAGGCAAACCTCAAATAAAAATG
  ACAAAGAAAACTGATCCCAGTGGACTATCTAAAACAATAATCTGCCATGTGGAAGGTTTT
  CCAAAGCCAGCCATTCAATGGACAATTACTGGCAGTGGAAGCGTCATAAACCAAACAGAG
  GAATCTCCTTATATTAATGGCAGGTATTATAGTAAAATTATCATTTCCCCTGAAGAGAAT
  GTTACATTAACTTGCACAGCAGAAAACCAACTGGAGAGAACAGTAAACTCCTTGAATGTC
  TCTGCTATAAGTATTCCAGAACACGATGAGGCAGACGAGATAAGTGATGAAAACAGAGAA
  AAGGTGAATGACCAGGCAAAACTAATTGTGGGAATCGTTGTTGGTCTCCTCCTTGCTGCC
  CTTGTTGCTGGTGTCGTCTACTGGCTGTACATGAAGAAGTCAAAGACTGCATCAAAACAT
  GTAAACAAGGACCTCGGTAATATGGAAGAAAACAAAAAGTTAGAAGAAAACAATCACAAA
  ACTGAAGCCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001627
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001627.2, NP_001618.2
• RefSeq Size: 4760 bp
• RefSeq ORF: 1752 bp
• Locus ID: 214
• Cytogenetics: 3q13.11
• Domains: ig
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.