CYP4B1 (NM_001099772) Human Untagged Clone

CAT#: SC316766

CYP4B1 (untagged)-Human cytochrome P450, family 4, subfamily B, polypeptide 1 (CYP4B1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP4B1
• Synonyms: CYPIVB1; P-450HP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001099772, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGCCCAGCTTCCTCTCCCTGAGCTTCTCCTCCTTGGGCCTGTGGGCTTCTGGGCTGATCTTGGTCT
  TAGGCTTTCTCAAGCTCATCCACCTGCTGCTGCGGAGGCAGACGTTGGCTAAGGCTATGGACAAATTCCC
  AGGGCCTCCCACCCACTGGCTTTTTGGACATGCCCTCGAGATCCAGGAGACGGGGAGCCTGGACAAAGTG
  GTGTCCTGGGCCCACCAGTTCCCGTATGCCCACCCACTCTGGTTCGGACAGTTCATTGGCTTCCTGAACA
  TCTATGAGCCTGACTATGCCAAAGCTGTGTACAGCCGTGGGGACCCTAAGGCCCCTGATGTGTATGACTT
  CTTCCTCCAGTGGATTGGGAGAGGCCTGCTGGTTCTTGAGGGGCCCAAGTGGTTGCAGCACCGCAAGCTG
  CTCACACCTGGCTTTCATTATGATGTGCTGAAGCCCTATGTGGCCGTGTTCACTGAGTCTACACGTATCA
  TGCTGGACAAGTGGGAAGAGAAAGCTCGGGAGGGTAAGTCCTTTGACATCTTCTGCGATGTGGGTCACAT
  GGCGCTGAACACACTCATGAAGTGCACCTTTGGAAGAGGAGACACCGGCCTGGGCCACAGCAGGGACAGC
  AGCTACTACCTTGCAGTCAGCGATCTCACTCTGTTGATGCAGCAGCGCCTTGTGTCCTTCCAGTACCATA
  ATGACTTCATCTACTGGCTCACCCCACATGGCCGCCGCTTCCTGCGGGCCTGCCAGGTGGCCCATGACCA
  TACAGACCAGGTCATCAGGGAGCGGAAGGCAGCCCTGCAGGATGAGAAGGTGCGGAAGAAGATCCAGAAC
  CGGAGGCACCTGGACTTCCTGGACATTCTCCTGGGTGCCCGGGATGAAGATGACATCAAACTGTCAGATG
  CAGACCTCCGGGCTGAAGTGGACACATTCATGTTTGAAGGCCATGACACCACCACCAGTGGTATCTCCTG
  GTTTCTCTACTGCATGGCCCTGTACCCTGAGCACCAGCATCGTTGTAGAGAGGAGGTCCGCGAGATCCTA
  GGGGACCAGGACTTCTTCCAGTGGGATGATCTGGGCAAAATGACTTATCTGACCATGTGCATCAAGGAGA
  GCTTCCGCCTCTACCCACCTGTGCCCCAGGTGTACCGCCAGCTCAGCAAGCCTGTCACCTTTGTGGATGG
  CCGGTCTCTACCTGCAGGAAGCCTGATCTCTATGCATATCTATGCCCTCCATAGGAACAGTGCTGTATGG
  CCCGACCCTGAGGTCTTTGACTCTCTGCGCTTTTCCACTGAGAATGCATCCAAACGCCATCCCTTTGCCT
  TTATGCCCTTCTCTGCTGGGCCCAGGAACTGCATTGGGCAGCAGTTTGCCATGAGTGAGATGAAGGTGGT
  CACAGCCATGTGCTTGCTCCGCTTTGAGTTCTCTCTGGACCCCTCACGGCTGCCCATCAAGATGCCCCAG
  CTTGTCCTGCGCTCCAAGAATGGCTTTCACCTCCACCTGAAGCCACTGGGCCCTGGGTCTGGGAAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001099772
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099772.1, NP_001093242.1
• RefSeq Size: 2173 bp
• RefSeq ORF: 1539 bp
• Locus ID: 1580
• Cytogenetics: 1p33
• Protein Families: Druggable Genome, P450, Transmembrane
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).