TCF12 (NM_003205) Human Untagged Clone

CAT#: SC316820

TCF12 (untagged)-Human transcription factor 12 (TCF12), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF12
• Synonyms: bHLHb20; CRS3; HEB; HsT17266; HTF4; p64; TCF-12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_207038, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCCCCAGCAACAACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGACTTCA
  GTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAACTAGACCAACTACACTGGGAAGCAGTCAATTCAG
  TGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAACAAGTGGTCAACCAAGTCCTTCCTAT
  GATTCATCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAGCCC
  ATGAAGGCTTGTCCCCAACACCTTTCATGAACTCAAATCTGATGGGAAAAACATCAGAGAGAGGCTCATT
  TTCCCTGTACAGCAGAGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGACAAGATCTGGGGCTT
  GGGAGCCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTACAAGTT
  CCAGGAGGAGACCACTCCATGACTCTGCAGCGCTTGATCCCTTGCAAGCAAAAAAAGTCAGAAAGGTGCC
  TCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGATGATTTCAACCGTGAATCTCCTAGT
  TATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATT
  CTTCTGACCTTTGGAGTTCATCAAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCAC
  TTCCCACATGTCTCAATCCAGTAGTTATGGCAACCTTCATTCACATGACCGCTTGAGTTATCCTCCACAC
  TCAGTTTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCA
  GTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAGGAACCAGAGG
  GAATGCTGCTGGAAGCTCACAGACAGGTGATGCACTTGGAAAGGCTTTGGCATCTATTTATTCTCCTGAC
  CATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACCTCTCACAGGTACCA
  GTCAGTGGCCAAGACCTGGAGGGCAAGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGCA
  GTCTCGAATGGAGGATCGTTTAGACAGACTGGATGATGCAATCCATGTGCTGCGGAACCATGCTGTGGGA
  CCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACCAA
  TTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTTGG
  AACTCATCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAGTCCTGTCTAGTACAGTCACTACTTCA
  AGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAGGTGGCTTGCAAAGTCAGTCTGGAACTGTTG
  TTACAACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGATGA
  CATGAAGTCAGATGATGAATCCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTACT
  AATGAAGATGAGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTAACAATG
  CCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGTGTCAGCTTCA
  CTTGAAGAGTGAAAAACCCCAAACAAAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTCTA
  GAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAGCCTGCCTTAAGAGAAGGGAAGAAGAAAAAG
  TTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACCAA
  CCCTATGGGTCATATGTAA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_003205
• Insert Size: 4600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003205.3, NP_003196.1
• RefSeq Size: 4734 bp
• RefSeq ORF: 2049 bp
• Locus ID: 6938
• Cytogenetics: 15q21.3
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR and coding region (compared to variant 1), resulting in a protein that maintains the reading frame but is shorter, compared to isoform a. Variants 3 and 4 encode the same isoform b.