TLE3 (NM_001105192) Human Untagged Clone

CAT#: SC317130

TLE3 (untagged)-Human transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (TLE3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001105192, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTG
  AGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA
  CGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC
  TTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGC
  CTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGAC
  GGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCC
  CCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCT
  CCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCA
  TGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCC
  AGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACA
  GCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCC
  CGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAA
  AAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACC
  TTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCC
  AACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATA
  GCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGATGAGCCACC
  ATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGCCTCCACAACATCCCACCCCAGATGAG
  CGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTT
  GACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAGGAAAACCAG
  CGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGCCCTGGCAGG
  CCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGTGCCGTGACC
  ATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACATCAGCCAGC
  CAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTCCTGCAAGCT
  GCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCG
  CCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCATTAGCCCTG
  ACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCACAACCAGACCCT
  GGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGACATCTCCCATGATGGCACCAAACTG
  TGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTACAGCAGCATG
  ACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGGCATGGAGAG
  CAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGAGAGCTGCGTG
  CTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACTGGGAAAGATAACCTTCTCAACGCCT
  GGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTGTGACATTTC
  AGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTTTATGAGGTCATCTACTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001105192
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105192.2, NP_001098662.1
• RefSeq Size: 5751 bp
• RefSeq ORF: 2310 bp
• Locus ID: 7090
• Cytogenetics: 15q23
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.